Murat Taşdemir

High implantation and pregnancy rates with testicular sperm extraction and intracytoplasmic sperm injection in obstructive and non-obstructive azoospermia

Thirty-two infertile couples with obstructive and non-obstructive azoospermia were included in this study. Testicular sperm extraction (TESE) was performed in 16 obstructive azoospermic cases where microsurgical sperm aspiration (MESA) or percutaneous sperm aspiration (PESA) were impossible because of totally destroyed epididymis and 16 non-obstructive azoospermia cases with severe spermatogenetic defect where the testicles were the only source of sperm cells. A total of 288 oocytes was obtained from 32 females and 84% were injected. The fertilization rates (FR) with 2 pronuclei (PN) and cleavage rate were 50.8 and 68.2% respectively. A total of 15 pregnancies was achieved (53% per embryo transfer), nine from the obstructive and six from the non-obstructive group. Four pregnancies resulted in clinical abortion (26.6%). The ongoing pregnancy rate was 39.2% per embryo transfer (ET) and 34.3% per started cycle. A high implantation rate was also achieved (26.6% in non-obstructive and 30% in obstructive azoospermia group). Using testicular spermatozoa in combination with ICSI in both obstructive and non-obstructive azoospermic groups, high implantation and pregnancy rates can be achieved.

Effect of pentoxifylline on immotile testicular spermatozoa.

AbstractPurpose: A model for differentiating live and dead sperm cells during intracytoplasmic sperm injection (ICSI) is proposed. Methods: We used pentoxifylline, a phosphodiesterase inhibitor known to enhance sperm motility, to initiate motility in testicular spermatozoa. Ten immotile testicular sperm samples were divided into two parts for examination of sperm motility with and without pentoxifylline treatment at 30, 60, and 90 min. Results: The samples without pentoxifylline remained immotile even after 90 min of incubation: the addition of pentoxifylline initiated sperm motility in all samples: 51.8±10.2, 64.4±9.4, and 70.8±8.9% (mean±SD) at 30, 60, and 90 min, respectively. Conclusions: That pentoxifylline may be used to differentiate live testicular spermatozoa during ICSI, which may improve fertilization and pregnancy rates, is suggested.

Effect of vaginal misoprostol application for cervical softening in pregnancy interruption before ten weeks of gestation

Objective. To determine the effect of vaginal misoprostol application for cervical dilatation and to determine the lime taken for pregnancy interruption.

Sperm immobilization antibodies in infertile male sera decrease the acrosome reaction: A possible mechanism for immunologic infertility

AbstractObjective: To study the effect of sperm-immobilizing antibodies from male sera on spontaneous and A23187-induced acrosome reactions (AR). Design: Swim-up spermatozoa obtained from three fertile donors were incubated with 13 sera with sperm-immobilizing antibodies obtained from infertile men and three control sera obtained from healthy fertile males. Sperm acrosomes were examined by staining with pisum sativum agglutinin labeled with fluorescein isothiocyanate (30 µg/ml; Sigma Chemical Co., St. Louis, MO) as spontaneous and A23187 (used at a final concentration of 10 µM; Sigma Chemical Co.) induced. Results: The incidence of spontaneous AR of spermatozoa incubated with antisperm antibody positive male sera (6.2±0.7) was significantly (P<0.001) lower than that of spermatozoa incubated with control sera (10.7±0.5). And the incidence of A23187-induced and -inducible (incidence of induced minus spontaneous) ARs of spermatozoa incubated with sperm antibody-positive male sera (12.4±1.9 and 6.2±1.9) was significantly lower (P<0.001) than that of spermatozoa incubated with control sera (31.0±0.5 and 20.3±0.9). Sperm-immobilizing antibody-positive sera decreased spontaneous, A23187-induced, and inducible ARs. Conclusions: Sperm-immobilizing antibodies from male sera interfere with fertilization by inhibiting the AR.

Short Protocol of Gonadotropin Releasing Hormone Agonist Administration Gave Better Results in Long Protocol Poor-Responders in IVF-ET

Objective: To determine the effectiveness of a short protocol (SP) of gonadotropin‐releasing hormone agonist (GnRH‐a) administration in patients who already had undergone at least one in vitro fertilization (IVF) and embryo transfer (ET) treatment cycle with the long protocol (LP) and had shown poor results.

Effect of chlamydial antibodies on the outcome of in vitro fertilization (IVF) treatment.

ObjectiveOur objective was to study the relationship between the presence of Chlamydia trachomatisantibodies and the success of IVF treatment.DesignWe evaluated 118 in vitro fertilization and embryo transfer (IVF-ET) treatment cycles from 51 couples with a history of infertility lasting for at least 2 years. All women starting a treatment cycle had their serum chlamydial antibody titers measured by indirect immunofluorescent technique. All patients received similar ovarian stimulation regimens and the oocytes collected were inseminated with similar concentrations of motile sperm. Clinical data from couples where the female partner had C. trachomatisab titers ≥40 have been compared with the equivalent data from couples where the female partner had C. trachomatisab titers <40.ResultsThere was no statistically significant difference between the two groups concerning age, infertility period, oocytes collected, oocytes fertilized, and fertilization rate, and the pregnancy rates were comparable.ConclusionPrevious exposure to C. trachomatisdid not alter the success rate of IVF-ET.