Müşerref Tatman Otkun

P(4-VP) based nanoparticles and composites with dual action as antimicrobial materials

Polymeric 4-VP (p(4-VP)) particles were synthesized in an oil-in-water microemulsion system using various amounts of ethylene glycol dimethacrylate (EGDMA) as crosslinker. The prepared p(4-VP) particles were chemically modified to obtain positively charged particles as polyelectrolytes. Furthermore, these p(4-VP) particles were used for in situ Ag and Cu metal nanoparticle syntheses to provide dual action with an additional advantage as bactericidal particles. The synthesized p(4-VP) particles with positive charges and metal constituents were tested for potential antibacterial action against various bacteria such as Staphylococcus aureus ATCC6538, Pseudomonas aeruginosa ATCC9027, Bacillus subtilis ATCC6633, Escherichia coli ATCC8739. It was found that p(4-VP) particles, especially the positively charged forms had potential as antibacterial materials. The synthesized particle dimensions were characterized with TEM, and DLS measurements. Chemical modification of the particles was confirmed by FT-IR spectroscopy and zeta potential measurements, and the metal nanoparticle contents were determined with thermogravimetric (TGA) studies.

Novel hydrogel particles and their IPN films as drug delivery systems with antibacterial properties

Poly(acrylonitrile) (p(AN))-based materials such poly(acrylonitrile-co-(3-acrylamidopropyl)-trimethylammonium chloride (p(AN-co-APTMACl)), poly(acrylonitrile-co-4-viniyl pyridine) (p(AN-co-4-VP)) and poly(acrylonitrile-co-N-isopropylacrylamide) (p(AN-co-NIPAM)) core-shell nanoparticles were prepared. The core materials, AN, in p(AN-co-4-VP) nanoparticles, were amidoximated and the shell materials, 4-VP, were quaternized to generate p(AN-co-4-VP)(+) and p(AN-co-4-VP)(++), single and double positively charged core-shell nanoparticles, respectively. Furthermore, interpenetrating microgels-hydrogel (IPN) polymeric networks were prepared by mixing double quaternized p(AN-co-4-VP)(++) core-shell particles with acrylamide (AAm) and 2-hydroxyethylmethacrylate (HEMA) before polymerization. A model drug, fluorescein sodium salt (FSS) was used in absorption/release studies from these IPNs. Moreover, the prepared and chemically modified particles were tested against Staphylococcus aureus ATCC6538, Pseduomonas aeruginosa ATCC9027, Bacillus subtilis ATCC6633, and Escherichia coli ATCC8739, and found that some of these particles had antibacterial properties against tested bacteria.

Relationship between Helicobacter pylori Adenotonsillar Colonization and Frequency of Adenotonsillitis in Children.

BACKGROUND There are insufficient data in the literature on the presence of Helicobacter pylori in tonsil and adenoid tissue of patients with only airway obstruction. This study examined the presence of H. pylori in surgical cases with airway obstruction or recurrent infection. AIMS To investigate the relationship between H. pylori adenotonsillar colonisation and the frequency of adenotonsillitis and to compare paediatric and adult patients according to H. pylori tonsillar colonisation. STUDY DESIGN Prospective clinical trial. METHODS PATIENTS SCHEDULED FOR ADENOIDECTOMY OR TONSILLECTOMY WERE CLASSIFIED INTO THREE GROUPS BASED ON INDICATIONS: paediatric infection (n=29), paediatric obstruction (n=29) and adult infection (n=12). Tissue samples obtained from patients were examined for the presence of H. pylori by culture, rapid urease test and polymerase chain reaction. RESULTS Forty-nine tonsil tissues were examined. Positive results were found in two specimens with the rapid urease test (4.1%) and three with polymerase chain reaction examination (6.1%). Only three positive polymerase chain reaction results (5.8%) were identified in 52 adenoid tissue samples. There were no statistically significant differences in the presence of H. pylori between paediatric infection and obstruction groups or between paediatric infection and adult infection groups. CONCLUSION In our study, there was a low incidence of H. pylori colonisation in tonsil and adenoid tissues. Regarding H. pylori colonisation, there was no significant difference between paediatric infection and obstruction groups. Also, no significant difference was found between adult and paediatric cases.

Evalution of Antibacterial Activities of Some Antiseptics and Disinfectants

Objective: In our study, we aimed to investigate the effects and duration of effect of 4 antiseptics and disinfectants frequently used in hospitals on 12 different resistant species of bacteria that cause nosocomial infections which were isolated from inpatients in our hospital. Methods: Three strains each of Acinetobacter baumannii, Pseudomonas aeruginosa, methicillinresistant Staphylococcus aureus and vancomycin-resistant Enterococcus were chosen for the study. Three American Type Culture Collection standard bacteria strains (ATCC) including P.aeruginosa ATCC 27853, Escherichia coli ATCC 25922, and Staphylococcus aureus ATCC 6538 were used as the control group. Antiseptics and disinfectants were evaluated at different concentrations and included povidone iodine (10%), sodium hypochloride (5%), ethyl alcohol and gluteraldehyde (2%) which are routinely used for disinfection in our hospital. Povidone iodine (10%) and gluteraldehyde (2%) were evaluated in 3 forms; undiluted according to the manufacturer’s instructions and also in 1/2 and 1/4 diluted forms. Sodium hypochlorite (5%) was examined in undiluted form, and at 1/10 and 1/100 dilution. Ethyl alcohol was prepared at the concentrations of 95%, 70%, and 50% before use. The selected bacteria ÖZET Amaç: Çalışmamızda hastanelerde sık kullanılan 4 adet antiseptik ve dezenfektanın hastanemizde yatan hastalardan izole edilen hastane enfeksiyonu etkeni, dirençli ve farklı cinslerden 12 adet bakteri üzerine etkisi ve etki süresinin incelenmesi amaçlanmıştır. Yöntem: Acinetobacter baumannii, Pseudomonas aeruginosa, metisiline dirençli Staphylococcus aureus, vankomisin dirençli Enterococcus bakterilerinden bu çalışma için 3’er tane seçildi. Kontrol için 3 adet American Type Culture Collection (ATCC) standart bakteri suşu (P. aeruginosa ATCC 27853, Escherichia coli ATCC 25922, S. aureus ATCC 6538) kullanılmıştır. Antiseptik ve dezenfektanlardan hastanelerde en sık kullanılan povidon-iyot (%10), sodyum hipoklorit (%5), etil alkol ve glutaraldehit (%2) çalışmamıza alındı. Povidon-iyot (%10) ve glutaraldehit (%2) firmanın kullanım talimatına göre direkt, ek olarak 1/2 ve 1/4 sulandırılarak 3 şekilde kullanıldı. Sodyum hipoklorit (%5) direkt, 1/10 ve 1/100 sulandırımlarda hazırlandı. Etil alkol kullanım öncesi %95-%70-%50’lik konsantrasyonda hazırlandı. Seçilen bakteriler kalitatif süspansiyon test yöntemine göre 1, 2, 5, 10, 30 dakikalık 1Çanakkale Onsekiz Mart Üniversitesi, Sağlık Uygulama ve Araştırma Hastanesi, Çanakkale 2Çanakkale Onsekiz Mart Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Çanakkale Geliş Tarihi / Received : Kabul Tarihi / Accepted : İletişim / Corresponding Author : Müşerref OTKUN Çanakkale Onsekiz Mart Üni. Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı Çanakkale Türkiye Tel : +90 535 355 16 56 E-posta / E-mail : otkun2000@yahoo.com DOI ID : 10.5505/TurkHijyen.2017.75002 Türk Hijyen ve Deneysel Biyoloji Dergisi Avcı D, Otkun M. Bazı antiseptik ve dezenfektanların antibakteriyel etkinliklerinin araştırılması. Turk Hij Den Biyol Derg, 2017; 74(3): 211-220 31.05.2016 10.03.2017 Makale Dili “Türkçe”/Article Language “Turkish”

The Effect of Systemic Carnitine Administration on Colon Anastomosis Healing in an Experimental Sepsis Model

ABSTRACT Aim: This study aimed to investigate the effects of L-carnitine, with its known antioxidant properties and positive effects on wound healing, on the healing of colon anastomosis in a cecal ligation and puncture sepsis model in rats. Materials and Methods: A total of 40 Sprague-Dawley rats were used. The rats were randomly divided into four groups of 10 (n = 10). Groups 1 and 2 had laparotomy and colon anastomosis performed. Groups 3 and 4 had cecal ligation and puncture (CLP) and colon anastomosis performed. Rats in Groups 1 and 3 were given 15 mL/kg intraperitoneal 0.9% isotonic NaCl, while the rats in Groups 2 and 4 were given 100 mg/kg intraperitoneal L-carnitine. On the postoperative fifth day, the rats were sacrificed and the burst pressure of anastomosis, histopathological effects, and tissue hydroxyproline levels were investigated. Results: In Groups 2 and 4, the anastomosis burst pressure and histopathological results in both noninfected abdomen and presence of peritonitis were statistically high compared to the control group (p < 0.05). Though the hydroxyproline levels were also high, there was no statistically significant difference for this parameter (p > 0.05). Conclusions: The findings obtained from the experimental sepsis model showed that healing of anastomosis in both the presence of peritonitis and noninfected abdomen was positively affected by the systemic administration of L-carnitine and this may contribute to the safety of anastomosis.

Evaluation of tuberculosis laboratory results in Canakkale Onsekiz Mart University Research and Education Hospital for 2009-2011

Objective: Tuberculosis microbiological laboratory diagnosis was firstly started in year 2009, in Microbiology Laboratory of Onsekiz Mart University Research and Education Hospital in Canakkale. We aimed at this study to present our laboratory data and to evaluate the methods which were used for the diagnosis of micobacteria. Method: Samples sent to our laboratory for tuberculosis culture were stained by Ehrlich-ZiehlNeelsen (EZN) method and evaluated microscopically. After processing of samples, each sample was inoculated to Lowenstein-Jensen medium (LJ) and BACTEC MGIT 960 (Mycobacteria Growth Indicator Tube, Becton Dickinson, USA) liquid based medium. If suspected growth was detected, Mycobacterium tuberculosis complex (MTBC) typing was made and if requested antituberculosis drug susceptibility for streptomycin (STR), isoniazid (INH), rifampicin (RF) and ethambutol (ETM) tested. Samples from normally sterile body sites cultured directly, others were firstly decontaminated and concentrated. Results: During the study period 1.048 samples from 667 patient has been processed. Seventy eight samples (7.44%) from 54 patients were found positive OZET Amac: Canakkale Ilinde tuberkuloz kultur

Ocular aspergillosis: Obtaining a specimen is crucial for diagnosis. A report of three cases.

Aspergillus species have been implicated in a wide variety of primary ocular conditions, characterized by either slow and asymptomatic infection, or rapid, uncontrollable progression and sometimes death. The study design is a case series including chronic asymptomatic dacryocystitis, dacryocystitis with dacryoliths and endogenous endophthalmitis. Due to this variable clinical presentation of aspergillosis a high level of suspicion must be maintained as fulminant aspergillosis may lead to a rapidly progressive infection which may result in death.