Mutsuko Konno

A possible association between hepatitis-B antigen-negative infantile papular acrodermatitis and Epstein-Barr virus infection

lymph-node cells and hepatocytes in papular acrodermatitis of childhood. J Cutan Pathol 2:97, 1975. 11. Takata M, Fukui Y, Taketani T, and Nakamura Y: Papular aerodermatitis of childhood (Gianottis disease), A report of two cases, J Dermatol 7:357, 1980. 12. Holland PV: Hepatitis B antigen subtypes--history, significance and immunogenicity, Am J Med Sci 270:161, 1975. 13. Mazzur S, Burgert S, and Blumberg BS: Geographical distribution of Australia antigen determinants d, y and w, Nature 247:38, 1974. 14. lshimaru Y, Ishimaru H, Toda G, Baba K, and Mayumi M: An epidemic of infantile papular acrodermatitis (Gianottis disease) in Japan associated with hepatitis-B surface antigen subtype ayw, Lancet 1:707, 1976. 15. Colombo M, Gerber MA, Vernage S J, Gianotti F, and Paronetto F: Immune response to hepatitis B virus in children with papular acro-dermatitis, Gastroenterology 73:1103, 1977. 16. Gianotti F: The Gianotti-Crosti syndrome, JCE Dermatol 18:15, 1979.

Detection of Human Bocaviruses 1 to 4 from Nasopharyngeal Swab Samples Collected from Patients with Respiratory Tract Infections

ABSTRACT Human bocaviruses (HBoV) 1, 2, 3, and 4 (HBoV1-4) were detected in 132 (15.5%), 5 (0.6%), 3 (0.4%), and 5 (0.6%) of 850 nasopharyngeal swab samples collected from children with respiratory tract infections, respectively. Out of the 145 HBoV1-4-positive samples, 62 (42.8%) were codetected with other respiratory viruses.

Comparison of a Lateral-Flow Immunochromatography Assay with Real-Time Reverse Transcription-PCR for Detection of Human Metapneumovirus

ABSTRACT A lateral-flow immunochromatography (IC) assay for the detection of human metapneumovirus (hMPV) has been developed by using two mouse monoclonal antibodies to the nucleocapsid protein of hMPV. The purpose of this study was to compare the virus detection rate in nasopharyngeal secretions by the IC assay with that by real-time reverse transcription-PCR (RT-PCR). We collected nasopharyngeal swab samples from 247 children with respiratory symptoms in Sapporo, Japan, during the period from April to July 2007. Sixty-eight of the 247 children were positive for hMPV by real-time RT-PCR. When the real-time RT-PCR was used as the reference standard, the IC assay results were positive for 48 of the 68 real-time RT-PCR-positive children (70.6% sensitivity) and 8 of the 179 real-time RT-PCR-negative children (95.5% specificity). Although the sensitivity of the IC assay is lower than that of real-time RT-PCR, the IC assay is a rapid and useful test for the diagnosis of hMPV infections in children.

Detection of Epstein-Barr Virus Sequences in Patients with Kawasaki Disease by Means of the Polymerase Chain Reaction

We used a selective DNA amplification technique to detect Epstein-Barr virus (EBV) DNA in peripheral blood mononuclear cells from patients with Kawasaki disease (KD). By means of the polymerase chain reaction EBV sequences were indentified directly in 21 (60%) of 35 KD patients within 2 weeks after the onset of KD. Furthermore, EBV sequences were detected in all of 6 repeatedly tested patients with KD within 3 months after disease onset. In contrast, only 2 (12%) of 17 control DNA samples were polymerase chain reaction positive. These results indicate that an unusual EBV-cell interaction may exist in KD.