Mutsuya Takeuchi

Comparative studies on the distribution of the glycopeptide and glycosaminoglycans in the human abdominal walls

Abstract 1. 1. The glycopeptide and glycosaminoglycans in the different layers of the human abdominal walls were assayed by determining the carbohydrate content of materials which remained after proteolysis. Values were highest in the skin and lowest in the muscle. 2. 2. Cellulose acetate strip electrophoresis of concentrates revealed the presence of three alcian blue positive fractions in every case. Although the proportion of the fraction which corresponded in mobility with hyaluronic acid was highest in the muscle, that of the fraction which corresponded in mobility with chondroitin sulfates and heparin was highest in the fascia.

Carbohydrate composition of glycopeptides from the human cerumen.

Abstract Five glycopeptides and two glycosaminoglycans were obtained from the protease-digested human cerumen. Separation of these seven polymers was achieved by stepwise elution from DEAE-cellulose with increasing concentration of NaCl. All the isolated glycopeptide preparations contained galactose, glucose, mannose, fucose, glucosamine, galactosamine, sialic acid, and peptide. Sulfate was found in both fractions 0.2 and 0.45 m NaCl, and uronic acid in fraction 0.45 m in addition to the above constituents.

Glycopeptide and glycosaminoglycans in the process of hepatic cancer induced by 3'-methyl-DAB.

Abstract 1. 1. A study of the glycopeptide and glycosaminoglycans has been made on the process of hepatic cancer of rats induced by 3′-niethyl-4-dimethylaminoazobenzene for 3–35 weeks. 2. 2. Both the glycopeptide and glycosaminoglycans contents increased gradually, falling once to a low level and rose at last again with the lapse of time. Both carbohydrate components were higher in the cancer nodule than in liver tissue adjacent to the tumor nodule. 3. 3. The relative proportion of the glycopeptide fraction also elevated gradually, falling once to a low level and rose at last again.

Isolation and characterization of the glycopeptide from a pancreatic cyst

Abstract 1. 1. Three glycopeptides and a glycosaminoglycan were obtained from the protease-digested pancreatic cyst content. 2. 2. Separation of these four polymers was achieved by stepwise elution from DEAE-cellulose with increasing concentration of NaCl. 3. 3. All the isolated preparations contained galactose, glucose, mannose, fucose, glucosamine, galactosamine, sialic acid, sulfate and peptide. Uronic acid was found in fraction 0.45 M in addition to the above constituents.

The glycopeptides from the human, bovine and porcine stomachs

Abstract 1. 1. Glycopeptides were isolated from tryptic digests of human, bovine and porcine gastric mucosae. 2. 2. Fractionation of the glycopeptide by preparative zone electrophoresis on a Pevikon C-870 block, yielded one glycopeptide fraction from the mucosa of the human stomach, whereas from both mucosae of the bovine fourth stomach and swine stomach it yielded three glycopeptide fractions. 3. 3. They were characterized by an increase in sialic acid and sulfate as the mobility of Pevikon block electrophoresis increased.

Further studies on the glycopeptides from the human, bovine and porcine stomachs

Abstract 1. 1. Glycopeptides were isolated from tryptic digests of human, bovine and porcine gastric mucosae, and were assayed by determining the carbohydrate content of materials. 2. 2. Fractionation of the glycopeptide by DEAE-cellulose chromatography yielded two glycopeptide fractions at the molarity of 0.1 M and 0.05 M NaCl from the mucosa of the human stomach, whereas it yielded 5 glycopeptide fractions at the molarity of 0.01–0.45 M NaCl from both mucosae of the bovine fourth and porcine stomachs. 3. 3. They were characterized by an increase in sialic acid and sulfate as the molarity of NaCl increased. Hexose was main component and hexosamine was the next in each fraction from all materials.

The hexosamine and sulfate-containing components in the urine of normal children.

Abstract A study has been made of the hexosamine and sulfate composition of the nondialyzable urinary components. The glucosamine, galactosamine, and sulfate contents per milliliter of the nondialyzable materials were all higher in the age group 2–5 yr than in the other age groups. The daily excretions, however, were all lower in children than in adults.

Isolation and partial characterization of the glycosaminoglycans from the human greater omentum.

Abstract 1. 1. The glycosaminoglycan was studied in the greater omentum of normal human subjects. The glycosaminoglycan preparation was made by digesting the greater omentum with proteolytic enzymes followed by removal of enzyme and undigested protein and finally freeze-drying of the supernatant. The contents of uronic acid, glucosamine and galactosamine were determined on this preparation. 2. 2. The uronic acid, glucosamine and galactosamine contents of this material were 820 ± 215.4 μg, 822 ± 204.6 μg and 381 ± 157.3 μg (mean ± standard deviations) per g of dry tissue, respectively. 3. 3. Cellulose acetate strip-electrophoresis of the material in barbital buffer revealed the presence of three alcian blue positive fractions in every case. The proportion of the electrophoretic fraction with approximate mobility of chondroitin sulfates was 38 ± 12.3%, that of the fraction with approximate mobility of heparan sulfate was 30 ± 4.6% and that of the fraction with approximate mobility of hyaluronic acid was 32 ± 10.3%. respectively.

Isolation and characterization of glycosaminoglycans from equine gastric mucosa

Abstract 1. 1. Five glycosaminoglycans were isolated from tryptic digest of equine gastric mucosa, and were assayed by determining the carbohydrate content of materials. 2. 2. Separation of these five polymers was achieved by Dowex 1 × 2 column chromatography. They were identified as hyaluronic acid, heparan sulfate, chondroitin-4-sulfate, chondroitin-6-sulfate and dermatan sulfate, respectively.

Comparative studies on the distribution of the glycopeptide in the different parts of the digestive tract

Abstract 1. 1. The glycopeptide and glycosaminoglycans in the different parts of the digestive tracts of man and rabbit were assayed by determining the carbohydrate content of material which remained after proteolysis. 2. 2. The glycopeptide content was higher in the mucosa than in the muscular-serosa, and was highest in the rectum mucosae of both man and rabbit. 3. 3. The glycopeptide, which was stained with both alcian blue and PAS, was observed in the mucosae of the esophagus, large intestine and rectum, while it was not observed in the mucosa of the stomach.