N. André

Multi-instrument analysis of electron populations in Saturn's magnetosphere

We analyze the radial distribution of electron populations inside 20 R-s in Saturns magnetosphere, and we calculate moments for these populations by a forward modeling method using composite spectra produced by the CAPS/ELS (0.6 eV to 26 keV) and the MIMI/LEMMS (15 keV to 10 MeV) instruments on board Cassini. We first calculate and harmonize both data sets in physical units and apply corrections taking into account biases introduced by spacecraft interaction with the magnetospheric environment. We then test different bimodal isotropic electron distribution models, deciding on a model with two kappa distributions. We adjust our isotropic model to the flux composite spectra with a least square method to produce three sets of fluid parameters (density, temperature, spectral index) per electron population. The radial profiles are then analyzed, revealing a relevant boundary at 9 R-s in both thermal and suprathermal electron populations. Observed discontinuities in the moment profiles (sudden drop-off in cold density profile outside 9 R-s, hot electrons drop-off inside 9 R-s) coincide with the known outer edge of Saturns neutral OH cloud. Farther out, thermal electrons disappear completely beyond 15 R-s while suprathermal electrons are still observed in the middle and outer magnetosphere.

Titan's near magnetotail from magnetic field and electron plasma observations and modeling: Cassini flybys TA, TB, and T3

[1] The first close Titan encounters TA, TB, and T3 of the Cassini mission at almost the same Saturnian local time � 1030 and in the same spatial region downstream of Titan have enabled us to study the formation of the tail of its induced magnetosphere. The study is based on magnetic field and electron plasma observations as well as threedimensional modeling. Our most important findings are the following: (1) No crossings of a bow shock of Titan were observed, and all encounters occurred at high plasma b > 1 for

Caspase-8 activation independent of CD95/CD95-L interaction during paclitaxel-induced apoptosis in human colon cancer cells (HT29-D4)

Antimicrotubule agent-induced apoptosis was examined in the proliferating human colon cancer cell line HT29-D4. G2/M arrest and subsequent apoptosis were dose-dependent, both observed with 100 nM paclitaxel or docetaxel and 10 nM vinorelbine. Bcl-x(L) phosphorylation was observed simultaneously with mitotic block, then caspase-3 cleavage and poly(ADP-ribose) polymerase degradation were detected 48 hr later. By using both enzymatic assay and immunoblot detection of cleaved fragments, we showed that caspase-8, a central component of the CD95-induced apoptotic pathway, was significantly activated during paclitaxel exposure, contemporary to apoptosis occurrence. Caspase-8 activation and apoptosis were independent of CD95 ligation and evidenced only for concentrations inducing Bcl-x(L) phosphorylation and a decrease in mitochondria permeability. Similar results were obtained with docetaxel and vinca alkaloids. Thus, antimitotic drugs may induce apoptosis via caspase-8 activation independently of CD95/CD95-L. Caspase-8 may be a common mediator of anticancer drug-induced apoptosis that could represent a promising target for future therapies.

Properties of Saturn kilometric radiation measured within its source region

On 17 October 2008, the Cassini spacecraft crossed the southern sources of Saturn kilometric radiation (SKR), while flying along high-latitude nightside magnetic field lines. In situ measurements allowed us to characterize for the first time the source region of an extra-terrestrial auroral radio emission. Using radio, magnetic field and particle observations, we show that SKR sources are surrounded by a hot tenuous plasma, in a region of upward field-aligned currents. Magnetic field lines supporting radio sources map a continuous, high-latitude and spiral-shaped auroral oval observed on the dawnside, consistent with enhanced auroral activity. Investigating the Cyclotron Maser Instability (CMI) as a mechanism responsible for SKR generation, we find that observed cutoff frequencies are consistent with radio waves amplified perpendicular to the magnetic field by hot (6 to 9 keV) resonant electrons, measured locally.

Preliminary interpretation of Titan plasma interaction as observed by the Cassini Plasma Spectrometer: Comparisons with Voyager 1

The Cassini Plasma Spectrometer (CAPS) instrument made measurements of Titan s plasma environment when the Cassini Orbiter flew through the moon s plasma wake October 26,2004 (flyby TA) and December 13,2004 (flyby TB). Preliminary CAPS ion and electron measurements from these encounters (1,2) are compared with measurements made by the Voyager I Plasma Science Instrument (PLS). The comparisons are used to evaluate previous interpretations and predictions of the Titan plasma environment that have been made using PLS measurements (3,4). The plasma wake trajectories of flybys TA, TB and Voyager 1 are similar because they occurred when Titan was near Saturn s local noon. These similarities make possible direct, meaningful comparisons between the various plasma wake measurements. The inquiries stimulated by the previous interpretations and predictions made using PLS data have produced the following results from the CAPS ion measurements: A) The major ambient ion components of Saturn s rotating magnetosphere in the vicinity of Titan are H+, H2+, and O+. B) Finite gyroradius effects are apparent in ambient 0 as the result of its interaction with Titan s atmosphere. C) The principal pickup ions are composed of H+, H2+, CH4+ and N2+. D) There is clear evidence of slowing down of the ambient plasma due to pickup ion mass loading; and, as the ionopause~ is approached, heavier pickup ions such as N2+ become dominant. The similarities and differences between the magnitudes and structures of the electron densities and temperatures along the three flyby trajectories are described

Involvement of microtubules and mitochondria in the antagonism of arsenic trioxide on paclitaxel-induced apoptosis

Arsenic trioxide (As(2)O(3)) at low concentrations (1-10 microM) is effective in the treatment of acute promyelocytic leukemia (APL) and lymphoma and is in clinical trials for treatment of solid tumors. Paclitaxel, an antimicrotubule agent, is highly efficacious in the treatment of adult tumors and is in clinical evaluation in childhood tumors. This study is the first to investigate the combination of arsenic and paclitaxel in the range of clinically achievable concentrations. We found that the simultaneous combination was antagonistic on proliferation of the neuroblastoma SK-N-SH cell line by using the combination index (CI) method. Moreover, a 40+/-5% decrease in paclitaxel-induced apoptosis in cells co-treated with As(2)O(3) confirmed the antagonism. The mechanism of antagonism was studied at the cellular level with 200 nM paclitaxel, twice the IC(50) value, and with 1 microM As(2)O(3) which administered singly did not affect cell survival or the microtubule network. As(2)O(3) antagonized the effects of paclitaxel on tubulin and microtubules. Paclitaxel-induced mitotic block was decreased by 20+/-2% and bundles induced by 200 nM paclitaxel were less condensed in the presence of 1 microM As(2)O(3). As(2)O(3) (10-200 microM) induced a concentration-dependent inhibition of tubulin polymerization in vitro which was maintained in presence of paclitaxel. Spectrophotometric and spectrofluorometric measurements indicated an interaction of As(2)O(3) with tubulin SH groups, without modification of the stoichiometry of paclitaxel binding to tubulin. Moreover, 4 microM As(2)O(3) inhibited the release of cytochrome c from isolated mitochondria by 78+/-10%. Our results show that As(2)O(3) and paclitaxel act antagonistically on mitochondria and microtubules and illustrate the need for careful evaluation of drug combinations.

Tethys and Dione as sources of outward-flowing plasma in Saturn's magnetosphere.

Rotating at over twice the angular speed of Earth, Saturn imposes a rapid spin on its magnetosphere. As a result, cold, dense plasma is believed to be flung outward from the inner magnetosphere by centrifugal force and replaced by hotter, more tenuous plasma from the outer magnetosphere. The centrifugal interchange of plasmas in rotating magnetospheres was predicted many years ago and was conclusively demonstrated by observations in Jupiter’s magnetosphere, which—like that of Saturn (but unlike that of Earth)—is rotationally dominated. Recent observations in Saturn’s magnetosphere have revealed narrow injections of hot, tenuous plasma believed to be the inward-moving portion of the centrifugal interchange cycle. Here we report observations of the distribution of the angle between the electron velocity vector and the magnetic field vector (‘pitch angle’) obtained in the cold, dense plasma adjacent to these inward injection regions. The observed pitch-angle distributions are indicative of outward plasma flow and consistent with centrifugal interchange in Saturn’s magnetosphere. Further, we conclude that the observed double-peaked (‘butterfly’) pitch-angle distributions result from the transport of plasma from regions near the orbits of Dione and Tethys, supporting the idea of distinct plasma tori associated with these moons.

Thermal electron periodicities at 20RS in Saturn's magnetosphere

Cassini fields and particles observations show clear evidence of periodic phenomena in Saturns magnetosphere. Periodicities have been observed in kilometric radio emissions, total electron density (in the inner magnetosphere), magnetic fields, and energetic particles (in the outer magnetosphere). In this letter the first analysis of periodicities in thermal electron densities in Saturns outer magnetosphere are presented. Plasma sheet electron densities and temperatures at 20 +/- 2 R-S in Saturns magnetosphere are studied and examined as a function of SLS3 longitude. Evidence for a density minimum at 170 degrees is presented which is in excellent agreement with total electron density results in the 3-5 R-S range. The density asymmetry is interpreted as the result of a periodic plasma sheet motion where the northward offset of the plasma sheet varies with longitude hence producing a density modulation in the equatorial plane. The effect of magnetospheric compressions on the dayside density asymmetry are discussed.

On the cause of Saturn's plasma periodicity

Periodic plasma enhancements are examined for all Cassini orbits from December 29, 2005 through September 7, 2006. The events, which have UT durations of 3-4 hours, are centered near SLS3 longitude 10 degrees at radial distances near 15 R-S and at larger W longitudes at larger distances, reaching 180 degrees W by 49 R-S. Magnetic-field data within the events and outside 30 to 35 R-S show signatures of neutral-sheet crossings and magnetic reconnection (i.e., plasmoids). We conclude that plasmoids move outward from 30-35 R-S along a spiral path that rotates with the planet. The duration of these events is similar to that of SKR events, and they are ordered in the SKR-based SLS3 longitude system. A conceptual model, in which the plasmoids are triggered in the pre-midnight quadrant following (with a predictable delay) the appearance of SKR at the magnetopause and then propagate outward in a rotating spiral pattern, can explain the connection among periodicities observed in Saturns charged particles, magnetic fields, and kilometric radiation.

Auroral electron distributions within and close to the Saturn kilometric radiation source region

On 17 October 2008, Cassini observed for the first time the electron populations associated with the crossing of a Saturn kilometric radiation source region and its surroundings. These observations allow for the first time the constraint and quantification of the high-latitude acceleration processes, the current systems, and the origin of the low-frequency electromagnetic waves. Enhanced fluxes of field-aligned energetic electrons were measured by the Cassini electron plasma spectrometer in conjunction with unusual intense field-aligned current systems identified using the magnetometer instrument. In the region where downward field-aligned currents were measured, electron data show evidence of two types of upward accelerated electron beams: a broadband energetic (1-100 keV) electron population that is observed throughout the region and a narrow-banded (0.1-1 keV) electron population that is observed sporadically. In the regions where the magnetic field signatures showed evidence for upward field-aligned currents, we observe electron loss cone distributions and some evidence of shell-like distributions. Such nonthermal electron populations are commonly known as a potential free energy source to drive plasma instabilities. In the downward current region, the low-energy and energetic beams are likely the source of the very low frequency emissions. In the upward current region, the shell distribution is identified as a potential source for Saturn kilometric radiation generation via the cyclotron maser instability.