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N. Bernard

Université catholique de Louvain

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Featured researches published by N. Bernard.

FEBS Letters | 1991

Cloning of the D-lactate dehydrogenase gene from Lactobacillus delbrueckii subsp. bulgaricus by complementation in Escherichia coli

N. Bernard; Thierry Ferain; Dominique Garmyn; Pascal Hols; Jean Delcour

A strain of Escherichia coli (FMJ144) deficient for pyruvate formate lyase and lactate dehydrogenase (LDH) was complemented with a genomic DNA library from Lactobacillus delbrueckii subsp. bulgaricus. One positive clone showed LDH activity and production of D(−)lactate was demonstrated. The nucleotide sequence of the D‐LDH gene (ldhA) revealed the spontaneous insertion of an E. coli insertion sequence IS2 upstream of the gene coding region. The open reading frame encoded a 333‐amino acid protein, showing no similarity with known L‐LDH sequences but closely related to L. casei D‐hydroxyisocaproate dehydrogenase (D‐HicDH).

Applied and Environmental Microbiology | 1994

Use of homologous expression-secretion signals and vector-free stable chromosomal integration in engineering of Lactobacillus plantarum for alpha-amylase and levanase expression.

Pascal Hols; Thierry Ferain; Dominique Garmyn; N. Bernard; Jean Delcour

Journal of Bacteriology | 1994

Lactobacillus plantarum ldhL gene: overexpression and deletion.

Thierry Ferain; Dominique Garmyn; N. Bernard; Pascal Hols; Jean Delcour

Journal of Bacteriology | 1996

KNOCKOUT OF THE TWO LDH GENES HAS A MAJOR IMPACT ON PEPTIDOGLYCAN PRECURSOR SYNTHESIS IN LACTOBACILLUS PLANTARUM

Thierry Ferain; J N Hobbs; J Richardson; N. Bernard; Dominique Garmyn; Pascal Hols; N E Allen; Jean Delcour

FEBS Journal | 1994

NAD(+)-dependent D-2-hydroxyisocaproate dehydrogenase of Lactobacillus delbrueckii subsp. bulgaricus. Gene cloning and enzyme characterization.

N. Bernard; Keyji Johnsen; Thierry Ferain; Dominique Garmyn; Pascal Hols; J. John Holbrook; Jean Delcour

Biochemical and Biophysical Research Communications | 1995

D175 discriminates between NADH and NADPH in the coenzyme binding site of Lactobacillus delbrueckii subsp. bulgaricus D-lactate dehydrogenase.

N. Bernard; Keyji Johnsen; J. John Holbrook; Jean Delcour

Applied and Environmental Microbiology | 1995

Cloning, nucleotide sequence, and transcriptional analysis of the Pediococcus acidilactici L-(+)-lactate dehydrogenase gene.

Dominique Garmyn; Thierry Ferain; N. Bernard; Pascal Hols; Jean Delcour

FEBS Journal | 1997

D-2-hydroxy-4-methylvalerate dehydrogenase from Lactobacillus delbrueckii subsp. bulgaricus. II. Mutagenic analysis of catalytically important residues.

N. Bernard; Keyji Johnsen; Josep Lluís Gelpí; Josep A. Alvarez; Thierry Ferain; Dominique Garmyn; Pascal Hols; Antonio Cortés; Anthony R. Clarke; J. John Holbrook; Jean Delcour

Journal of Bacteriology | 1995