Nabil Ben Salem Abid
University of Monastir
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Nabil Ben Salem Abid.
Chemistry & Biodiversity | 2012
Zyed Rouis; Ameur Elaissi; Nabil Ben Salem Abid; Mohamed Ali Lassoued; Pier Luigi Cioni; Guido Flamini; Mahjoub Aouni
The chemical composition of the essential oils of five populations of Hypericum triquetrifolium Turra from Tunisia and their intraspecific variability were analyzed in detail by GC/MS. One hundred seventy‐four compounds were identified, representing averages of 87.9 to 98.7% of the oil composition. The components are represented here by homologous series of monoterpene hydrocarbons, oxygenated monoterpenes, sesquiterpenes hydrocarbons, oxygenated sesquiterpenes, non‐terpenic hydrocarbons, and others. Sesquiterpene hydrocarbons were the most abundant chemical compounds. Multivariate chemometric techniques, such as cluster analysis (CA) and principal‐component analysis (PCA), were used to characterize the samples according to the geographical origin. By statistical analysis, the analyzed populations were classified into four chemotype groups.
journal of applied pharmaceutical science | 2012
Nabil Ben Salem Abid; Zyed Rouis; Faten Nefzi; Nidhal Souelah; Mahjoub Aouni
The natural products are widely used in many fields as pharmaceutical, flavor, industrial, and additive compounds. In many cases, there is a need to evaluate the cytotoxic effect of these products and to determine the non cytotoxic concentration at which they can be used in a safe manner. The objective of the present study was to compare two methods of evaluation the cytotoxic effect of a plant extract. The MTT assay and the PI dye-inclusion assay were used to carry out the experiments. The results were then evaluated by calorimetric and flow cytometry assays. The obtained results showed that a clear difference between the two methods and the cell death using the MTT assay seem to be overestimated. To better evaluate the cytotoxic effect of natural product, we need to carry out several in vitro and in vivo tests before the judgment that a compound is safe to use and at which concentration.
Journal of Medical Virology | 2012
Hanen Boukoum; Imen Nahdi; Nabil Ben Salem Abid; Vincent Foulongne; Wissal Sahtout; D. Zallema; Habib Skiri; Sabra Aloui; Abdelatif Achour; Michel Segondy; Mahjoub Aouni
The polyomavirus JC (JCPyV) is a ubiquitous virus in humans, causing progressive multifocal leukoencephalopathy, a fatal demyelinating disease. JCPyV propagates in the adult kidney and excretes its progeny in urine, from which its DNA can be recovered readily. JCPyV isolates worldwide can be classified into 14 subtypes or genotypes, each associated with a specific geographical region. The European genotypes EU‐a‐b‐c are spread throughout Europe and Mediterranean areas. The major African genotype Af2 is spread not only throughout Africa but also in West and South Asia. A minor African genotype (Af1) occurs in Central and West Africa. Partially overlapping domains in Asia were occupied by various genotypes (e.g., B1‐a, ‐b, ‐d, B2, CY, MY, and SC). To characterize the subtypes of JCPyV prevalent in Tunisia, the presence of the virus was investigated by real‐time PCR in urine samples from 98 renal transplant recipients. For subtype identification, a 610 bp typing region of the JCPyV genome was amplified from each urine sample, and its DNA sequence was determined. In the patients studied, the major African subtype Af2 was the predominant (62.5%), followed by the European subtype EU (33.5%). Only one case clustering with the Asian genotype SC (4%) was identified. The presence of the European subtype with high prevalence in this population suggests that the epidemiological distribution of JCPyV virus sequences in North Africa is related partially to the epidemiological data in Europe. J. Med. Virol. 84:1818–1824, 2012.
Indian Journal of Virology | 2012
Hakima Gharbi-Khelifi; Nabil Ben Salem Abid; Abir Beji; Leila Bhiri; Rafik Harrath; Khira Sdiri; Sylviane Billaudel; Virginie Ferré; Mahjoub Aouni
The aim of the present study was to investigate the seroprevalence of Hepatitis A virus antibodies in patients with clinical symptoms of viral hepatitis and molecular characterization of the detected isolates. The present study deals with the seroprevalence and the genetic diversity of HAV in 400 Tunisian patients presenting in dispensaries (160 patients) and in University Hospitals (240 patients) with hepatitis symptoms between 2006 and 2008. The patients with acute hepatitis were mainly from rural regions. However, the total number of patients was decreased over time. The collected samples were from patients with hepatitis symptoms occurring mainly during January–March (36.7, 26, and 35.5%) and September–December (39.4, 43.4, and 35.5%) during the three years of study, respectively. However, HAV infection was established for only 110 among 400 patients. The detected isolates were clustered within sub-genotype IA. The present study constituted another report of the continued surveillance of HAV infection in the region of Monastir and the molecular characterisation of the detected strains.
BMC Public Health | 2015
Abir Monastiri; Mahjoub Aouni; Susana Guix; Badereddine Mechri; Marc López-Roig; Nabil Ben Salem Abid; Neji Gueddiche; Sabeur Hamami; Lamjed Boughzala; Jordi Serra-Cobo
Background/aimsAstroviruses (AstVs) are enteric viruses that can cause gastroenteritis in children. This study is part of monitoring the circulation of astroviruses in children hospitalized and/or outpatients for acute gastroenteritis at the primary care center of Ouerdanine or at the Pediatric Department of the University Hospital Fattouma-Bourguiba (Monastir, Tunisia). The aims of our study were to know the prevalence of human astrovirus in clinical samples of children, characterize the strains and evaluate the infectivity of isolated strains on cell culture.MethodsFifty stool samples were collected from children under five years old in the region of Monastir (Tunisia) from October 2010 to June 2011. All specimens were subjected to RT-PCR amplification followed by sequencing and phylogenetic analysis.ResultsThe study shows a low prevalence of astrovirus (4 %) in children. The two positive samples obtained were HAstV type 3. Samples that were RT-PCR positive were cultured in CaCO-2 cells and the presence of infectious viral particles was confirmed. The phylogenetic analysis shows that the different HAstV-3 strains isolated in Tunisia are grouped into two clusters. The first cluster includes strains obtained in 2004, which belong to lineage HAstV-3a, while strains isolated in 2010 belong to lineage HAstV-3c.ConclusionsThis study is part of monitoring the circulation of astroviruses in children younger than five years old from Monastir region, Tunisia. The results show low prevalence (4 %). All genotyped samples belonged to lineage HAstV-3c, which could be presently emerging. Two different lineages have been isolated in Tunisia: HAstV-3a in 2004 and HAstV-3c in 2010.
Virus Genes | 2011
Nabil Ben Salem Abid; Sergei A. Chupin; Olga P. Bjadovskaya; Olga G. Andreeva; Mahjoub Aouni; Javier Buesa; Taufik Z. Baybikov; Larisa B. Prokhvatilova
Porcine respiratory coronavirus is related genetically to porcine transmissible gastroenteritis virus with a large deletion in S protein. The respiratory virus is a mutated form that may be a consequence of the gastroenteritis virus’s evolution. Intensive passages of the virus in its natural host may enhance the appearance of mutations and therefore may contribute to any attenuated form of the virus. The objective of this study was to characterize the porcine transmissible gastroenteritis virus TMK22 strain after passages in piglets from 1992 until 2007. A typical experimental infection, molecular characterization, and serological analysis were also carried out to further characterize and to evaluate any significant difference between strains. The sequence analysis showed two amino acid deletions and loss of an N-glycosylation site in transmissible gastroenteritis virus S protein after passages in piglets. Although these deletions were positioned at the beginning of the antigenic site B of S protein, no clinical differences were observed in piglets infected experimentally either with the native virus or the mutated one. Serological tests did not show any antibody reactivity difference between the two strains. In this article, we report that the S protein deletion did not affect the virus’s pathogenicity. The variety of the virus’s evolutionary forms may be a result, not only of the multiple passages in natural hosts, but also of other factors, such as different pathogens co-infection, nutrition, immunity, and others. Further studies need to be carried out to characterize the mutated strain.
Brazilian Journal of Microbiology | 2011
Hakima Gharbi-Khelifi; Nabil Ben Salem Abid; Khira Sdiri; Rafik Harrath; Abir Beji; Leila Bhiri; Sylviane Billaudel; Virginie Ferré; Mahjoub Aouni
In the present study, epidemiological survey and molecular characterization of hepatitis A virus during an outbreak in five Tunisian childcare centers in El-Mahres during October and November 2006 were carried out. Five well-water and five drinking water samples were included in the present study. Serological investigation and molecular characterization were carried out. All patients were IgM seropositive and the viral genome was detected in all clinical and well-water samples whereas it was not detected in drinking water from the five childcare centers. Sequence analysis showed that all Tunisian strains belong to sub-genotype IA. The genetic profile of the VP1/2A junction showed that the outbreak isolates underwent an amino acid substitution which was absent in viruss strains detected previously in Tunisia. Further studies need to be conducted to evaluate the emergence of the viruss strains in clinical and water samples and more epidemiological data need to be collected about the risk factors which may contribute to acute hepatitis.
Asian Pacific Journal of Tropical Medicine | 2012
Nabil Ben Salem Abid; Rouis Zyed; Mahjoub Aouni
OBJECTIVE To study the recombination events among enterovirus strains and the development of specific primers for the detection of enteroviruses in environmental samples. METHODS Nucleotide sequence analysis of enteroviruses deposited in the international database GenBank (www.ncbi.nlm.nih.gov/Genbank) was conducted to develop specific primers for the detection of these viruses. The specificity and sensitivity of the method were tested using coxackievirus B3 strain Nancy, environmental isolate of human hepatitis A virus and human rotavirus strain WA. Seventy sewage samples were analyzed. RESULTS Enterovirus genome was detected in all positive samples. The genome of enterovirus was not detected in negative samples. The level of detection of these viruses was 10(2) TCID(50)/mL. CONCLUSIONS The development of new primers is an important issue for the detection of enteroviruses in the environment and the assessment of risk factors to human health.
BMC Complementary and Alternative Medicine | 2013
Zyed Rouis; Nabil Ben Salem Abid; Sadok Koudja; Thabet Yangui; Ameur Elaissi; Pier Luigi Cioni; Guido Flamini; Mahjoub Aouni
Environmental Science and Pollution Research | 2015
Karima Bekir; Houcine Barhoumi; Mohamed Braiek; Amani Chrouda; Nadia Zine; Nabil Ben Salem Abid; Abdelrazek Maaref; Amina Bakhrouf; Hafedh Ben Ouada; Nicole Jaffrezic-Renault; Hedi Ben Mansour