Naila Cristina Blatt Duda

Identification, occurrence and clinical findings of canine hemoplasmas in southern Brazil.

Hemoplasmas are ubiquitous pleomorphic and epicellular bacteria detected in erythrocytes in several species. In Brazil, studies on hemoplasmas have not included information on occurrence, clinical signs, and risk factors in dogs. This paper investigates the occurrence of hemoplasmas in dogs, focusing on risk factors and clinical status. Conventional PCR for the four types of canine hemoplasmas was performed in 331 blood samples collected from dogs clinically treated at a teaching veterinary hospital. Of all samples, 17/331 (5.1%) were positive for Mycoplasma haemocanis and 6/331 (1.8%) were positive for a Candidatus Mycoplasma haemominutum-like organism. Risk factors included the presence of vectors, old age, dog bite wounds, and neoplastic diseases. In the multivariate analysis, a 4.40 odds ratio in dogs with vector-borne diseases indicated risk for hemoplasmosis. There was correlation between hemoplasma infection and neoplastic disease, suggesting that neoplastic conditions are a risk factor for hemoplasma infection in dogs.

Ovariohisterectomia por videocirurgia (via NOTES vaginal híbrida), celiotomia ou miniceliotomia em cadelas

A ovariohisterectomia (OH) eletiva em cadelas e o procedimento mais realizado na cirurgia de pequenos animais. No presente trabalho, foram utilizados tres grupos de sete animais (GI, GII e GIII), submetidos a tres tecnicas de OH eletiva: por celiotomia (tecnica convencional), por miniceliotomia (tecnica do gancho de Snook), via cirurgia endoscopica transluminal por orificios naturais (NOTES) hibrida. Na comparacao, foram considerados o tempo de cirurgia, as complicacoes e dificuldades tecnicas trans e pos-operatorias, a avaliacao de dor pos-operatoria, a quantidade (g) de sangramento cirurgico estimado, alem da determinacao de determinados parâmetros vitais, como: frequencia cardiaca (FC), frequencia respiratoria (ƒ), temperatura retal, pressao arterial (media) invasiva (PAI) e a pressao venosa central (PVC). A OH via NOTES vaginal hibrida foi a tecnica que apresentou menor escore de desconforto pos-operatorio, menor sangramento cirurgico, porem com maior tempo cirurgico, comparado com as modalidade convencional e por miniceliotomia.

Phylodynamics of the Brazilian feline immunodeficiency virus

Feline immunodeficiency virus (FIV), like other retroviruses, displays large genomic divergence when different isolates are compared. In this study, 31 FIV positive samples of domestic cats from Porto Alegre, RS, Brazil were used aiming at a detailed genomic characterization and a better understanding of the molecular epidemiology of the virus in Brazil. The proviral env genes were partially amplified, sequenced and compared with another 237 sequences from different continents. We identified several Brazilian highly supported clades (A, B1, B2, C and D) that suggest independent events of introduction of FIV in Brazil. Forty six reference-sequences from the GenBank were used with our 31 sequences to infer the virus subtypes. Our sequences belong to the subtype B and three of them result from a recombination with the previously described subtype F. The other 28 Brazilian samples belonging to subtype B and another 46 Brazilian sequences from the GenBank were used to estimate the time to the most recent common ancestor of each Brazilian clade, using a Bayesian approach and a relaxed molecular clock model. The analyses of Brazilian sequences suggest several different entries of the virus in the Brazilian cat population between 1981 and 1991.