Nan Sun

Volume moiré tomography based on double cross gratings for real three-dimensional flow field diagnosis

Since the advantages of noncontact, strong antidisturbing capability and wide measurement range, moiré tomography has been considered a powerful diagnostic tool for flow fields. In this paper, the volume computerized tomography is introduced to obtain the real three-dimensional reconstruction based on moiré deflectometry. In order to realize volume moiré tomography (VMT), double cross gratings are applied in the moiré deflected system to gain the shearing phase distribution of the moiré deflected projection in two mutually perpendicular directions simultaneously. Thus, the scalar diffraction theory is used for analyzing the imaging process of VMT based on double cross gratings to achieve the explicit form of shearing phase. Finally, the real temperature distribution of a propane flame is reconstructed, which can confirm the VMT method.

Quantitative interferometric microscopy with improved full-field phase aberration compensation

Abstract. Single-shot quantitative interferometric microscopy (QIM) needs a high-accuracy and rapid phase retrieval algorithm. Retrieved phase distributions are often influenced by phase aberration background caused by both imaging system and phase retrieval algorithms. Here, we propose an improved phase aberration compensation (PAC) approach in order to eliminate the phase aberrations inherent in the data. With this method, sample-free parts are identified and used to calculate the background phase, reducing phase errors induced in samples and providing high-quality phase images. We now demonstrate that QIM based on this PAC approach realizes high-quality phase imaging from a single interferogram. This is of great potential for a real-time speedy diagnosis.

Unwrapping free Hilbert transform-based phase retrieval method in quantitative interferometric microscopy

Abstract. In order to obtain quantitative phase distributions from interferograms, phase retrieval composed of phase extracting and unwrapping is adopted in quantitative interferometric microscopy. However, phase unwrapping often requires a long time, limiting applications such as high-speed phase observations and measurements. In order to accelerate the processing speed, a phase unwrapping free Hilbert transform (HT)-based phase retrieval method is proposed. Though another background interferogram without a sample is needed, phase unwrapping can be omitted, saving a large amount of time for phase recovery. Additionally, the proposed HT-based method can maintain more sample details, thus providing high-accurate quantitative phase imaging. Considering its fast speed and high accuracy in phase retrieval, it is believed that the unwrapping free HT-based phase retrieval method can be potentially applied in high throughput cellular observations and measurements.

High-speed quantitative interferometric microscopy based phase imaging cytometer

The paper proposed a simple large scale bio-sample phase detecting equipment called gravity driven phase detecting cytometer, which is based on quantitative interferometric microscopy to realize flowing red blood cells phase distribution detection. The method has advantages on high throughput phase detecting and statistical analysis with high detecting speed and in real-time. The statistical characteristics of red blood cells are useful for biological analysis and disease detection. We believe this method is shedding more light on quantitatively measurement of the phase distribution of bio-samples.

Theoretical analysis for temporal phase-shifting of volume moiré tomography via double cross gratings

Volume Moiré Tomography (VMT) is an important technique to diagnose the flow field. In this Letter, the characteristic of temporal phase-shifting is analyzed for VMT. When the distance between two cross gratings is not on the Talbot distance, the phase-shifting factors are existed between moiré patterns of different orders. Especially, when the distance conforms to the sub-Talbot distance, the phase-shifting factors are maximum. This characteristic of temporal phaseshifting could be used for real 3-D flow fields reconstruction in the future.

Interferometric phase microscopy of red blood cells

Quantitative phase imaging of cells with high accuracy in a completely noninvasive manner is a challenging task. To provide a proper solution to this important need, interferometric phase microscopy is described which relies on the off-axis interferometry, confocal microscopy and high-speed image capture technology. Phase retrieval from the single interferogram is done by algorithms based on the fast Fourier transform, traditional Hilbert transform and two-step Hilbert transform, respectively. Furthermore, a phase aberrations compensation approach is applied to correct the phase distribution of the red blood cells obtained via the three methods mentioned before without the pre-known knowledge for removing the wave front curvature introduced by the microscope objectives, off-axis imaging, etc., which otherwise hinders the phase reconstruction. The improved results reveal the better inner structures of the red blood cells. The development of quantitative phase imaging technique is shedding light on their future directions and applications for basic and clinical research.

Theoretical analysis of volume moiré tomography based on double orthogonal gratings for real 3D flow fields diagnosis

Moiré tomography is an important technique to diagnose the flow field. However, the traditional moiré deflectometry cannot meet the requirements of Volume Moiré Tomography (VMT). In this Letter, an improved moiré deflected system based on double orthogonal gratings is introduced for real 3-D reconstruction. The proposed method could obtain the first-order partial derivatives in two vertical directions of the projection in one time. Comparing with the traditional moiré deflectometry, the proposed system is more effective and easier to realize the multi-direction data acquisition.