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Dive into the research topics where Nancy J. Bigley is active.

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Featured researches published by Nancy J. Bigley.


Journal of Immunology | 2009

HSV-1-Induced SOCS-1 Expression in Keratinocytes: Use of a SOCS-1 Antagonist to Block a Novel Mechanism of Viral Immune Evasion

Kenneth Gene Frey; Chulbul M. Ahmed; Rea Dabelic; Lindsey D. Jager; Ezra N. Noon-Song; S. Mohammad Haider; Howard M. Johnson; Nancy J. Bigley

Keratinocytes are important for the acute phase of HSV-1 infection and subsequent persistence in sensory nervous tissue. In this study, we showed that keratinocytes (HEL-30) were refractory to IFN-γ induction of an antiviral state to HSV-1 infection, while IFN-γ did induce an antiviral state in fibroblasts (L929). This led us to examine the possible role of suppressor of cytokine signaling-1 (SOCS-1) in this refractiveness. RT-PCR analysis of SOCS-1 mRNA expression in HSV-1-infected cells showed a 4-fold increase for keratinocytes while having a negligible effect on fibroblasts. A similar pattern was observed at the level of SOCS-1 protein induction. Activation of STAT1α in keratinocytes was inhibited by HSV-1 infection. A direct effect of HSV-1 on the SOCS-1 promoter was shown in a luciferase reporter gene assay. We have developed a small peptide antagonist of SOCS-1, pJAK2(1001–1013), that had both an antiviral effect in keratinocytes against HSV-1 as well as a synergistic effect on IFN-γ induction of an antiviral state. HSV-1 ICP0 mutant was inhibited by IFN-γ in HEL-30 cells and was less effective than wild-type virus in induction of SOCS-1 promoter. We conclude that SOCS-1 plays an important role in the inhibition of the antiviral effect of IFN-γ in keratinocytes infected with HSV-1. The use of SOCS-1 antagonist to abrogate this refractiveness could have a transformational effect on therapy against viral infections.


Science | 1960

Specific inhibition of RHo(D) antibody by sialic acids.

Matthew C. Dodd; Nancy J. Bigley; Virginia B. Geyer

Agglutination of RhO(D) erythrocytes by specific antiserum was inhibited by crude and crystalline N-acetylneuraminic acid, less inhibited by its glycoyl derivative, and weakly inhibited by its degradation product, N-acetyl-mannosamine, and by D-mannose. A brain ganglioside containing neuraminic acid and a Pseudomonas polysaccharide were even more inhibitory. Inhibition was specific for anti-D sera.


Cellular and Molecular Life Sciences | 1969

Autoantibodies in Canine Neoplasms. I. Reactivity of Autologous 7 S γ-Globulins with Tumor Cells

L. E. Yurko; Nancy J. Bigley; G. P. Wilson

Durch Immunofluoreszenz wird eine spezifische Bindung autologerγ-Globuline an neoplastische Zellen des Hundes nachgewiesen.


Cellular and Molecular Life Sciences | 1969

Autoantibodies in Canine Neoplasms. II. Tumor Tissue Specificity and Lack of Cross-Reactivity with Embryonic Antigens

L. E. Yurko; Nancy J. Bigley

Durch Immunofluoreszenz wird eine Kreuzreaktion zwischen Tumoren des gleichen histologischen Typs, jedoch keine solche zwischen verschiedenartigen Tumoren des Hundes festgestellt.


Journal of Immunology | 1963

The Immunologic Specificity of Antibodies to Liver Ribosomes and Nuclei

Nancy J. Bigley; Matthew C. Dodd; Virginia B. Geyer


Science | 1967

Gastrin Antibodies: Induction, Demonstration, and Specificity

D. R. Schneider; Gerald L. Endahl; Matthew C. Dodd; Jesseph Je; Nancy J. Bigley; Robert M. Zollinger


Nature | 1964

Chemical Aspects of Inhibitors of Rh 0 (D) Antibody

Matthew C. Dodd; Nancy J. Bigley; Garland A. Johnson; Robert H. McCluer


Science | 1962

Autoimmune response in rabbits injected with rat and rabbit liver ribosomes.

Matthew C. Dodd; Nancy J. Bigley; Virginia B. Geyer; Francis W. McCoy; Henry E. Wilson


Journal of Immunology | 1963

Further Observations on the Serologic Specificity of Sialic Acid for Rho(D) Antibody

Matthew C. Dodd; Nancy J. Bigley; Virginia B. Geyer


Journal of Immunology | 1958

Inhibition of Rh 0 (D) Antibody by Eluates from Rh 0 (D) Erythrocytes Treated with Mumps Virus or the Periodate Ion

Nancy J. Bigley; Robert W. Chandler; Matthew C. Dodd

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