Nancy Marcantonio
Yale University
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Nancy Marcantonio.
The Journal of Infectious Diseases | 2001
Subrata Das; Gautam Banerjee; Kathleen DePonte; Nancy Marcantonio; Fred S. Kantor; Erol Fikrig
Rabbits or guinea pigs infested with Ixodes scapularis acquire resistance to tick bites, a phenomenon, known as tick immunity, that is partially mediated by antibody. To determine the salivary gland antigens that elicit antibodies in the host, an I. scapularis salivary gland cDNA expression library was probed with serum from tick-immune rabbits. Sera from sensitized rabbits strongly recognized 47 of 100,000 library clones in an antibody-screening assay. These 47 clones encoded 14 different I. scapularis genes, including a glutathione peroxidase homologue. Expression of these 14 genes in engorged tick salivary glands was confirmed by reverse-transcription polymerase chain reaction. The I. scapularis glutathione peroxidase homologue, named salp25D, was expressed in both unfed and fed nymphal salivary glands. Recombinant Salp25D was able to catalyze the reduction of hydrogen peroxide in the presence of reduced glutathione and glutathione reductase. These results categorize the prominent salivary gland proteins in I. scapularis and demonstrate the presence of a potent antioxidant in tick saliva.
Journal of Experimental Medicine | 2006
Bindu Sukumaran; Sukanya Narasimhan; John F. Anderson; Kathleen DePonte; Nancy Marcantonio; Manoj N. Krishnan; Durland Fish; Sam R. Telford; Fred S. Kantor; Erol Fikrig
Anaplasma phagocytophilum is the agent of human anaplasmosis, the second most common tick-borne illness in the United States. This pathogen, which is closely related to obligate intracellular organisms in the genera Rickettsia, Ehrlichia, and Anaplasma, persists in ticks and mammalian hosts; however, the mechanisms for survival in the arthropod are not known. We now show that A. phagocytophilum induces expression of the Ixodes scapularis salp16 gene in the arthropod salivary glands during vector engorgement. RNA interference–mediated silencing of salp16 gene expression interfered with the survival of A. phagocytophilum that entered ticks fed on A. phagocytophilum–infected mice. A. phagocytophilum migrated normally from A. phagocytophilum–infected mice to the gut of engorging salp16-deficient ticks, but up to 90% of the bacteria that entered the ticks were not able to successfully infect I. scapularis salivary glands. These data demonstrate the specific requirement of a pathogen for a tick salivary protein to persist within the arthropod and provide a paradigm for understanding how Rickettsia-like pathogens are maintained within vectors.
PLOS ONE | 2007
Sukanya Narasimhan; Kathleen DePonte; Nancy Marcantonio; Xianping Liang; Thomas E. Royce; Kenneth Nelson; Carmen J. Booth; Benjamin Koski; John F. Anderson; Fred S. Kantor; Erol Fikrig
In North America, the black-legged tick, Ixodes scapularis, an obligate haematophagus arthropod, is a vector of several human pathogens including Borrelia burgdorferi, the Lyme disease agent. In this report, we show that the tick salivary gland transcriptome and proteome is dynamic and changes during the process of engorgement. We demonstrate, using a guinea pig model of I. scapularis feeding and B. burgdorferi transmission, that immunity directed against salivary proteins expressed in the first 24 h of tick attachment — and not later — is sufficient to evoke all the hallmarks of acquired tick-immunity, to thwart tick feeding and also to impair Borrelia transmission. Defining this subset of proteins will promote a mechanistic understanding of novel I. scapularis proteins critical for the initiation of tick feeding and for Borrelia transmission.
Infection and Immunity | 1992
Erol Fikrig; Stephen W. Barthold; Nancy Marcantonio; Kathleen DePonte; Fred S. Kantor; Richard A. Flavell
Proceedings of the National Academy of Sciences of the United States of America | 2004
Sukanya Narasimhan; Ruth R. Montgomery; Kathleen DePonte; Christian Tschudi; Nancy Marcantonio; John F. Anderson; John R. Sauer; Michael Cappello; Fred S. Kantor; Erol Fikrig
Cell Host & Microbe | 2007
Sukanya Narasimhan; Bindu Sukumaran; Ulas Bozdogan; Venetta Thomas; Xianping Liang; Kathleen DePonte; Nancy Marcantonio; Raymond A. Koski; John F. Anderson; Fred S. Kantor; Erol Fikrig
American Journal of Tropical Medicine and Hygiene | 1998
S Nazario; S Das; A M de Silva; Kathleen DePonte; Nancy Marcantonio; John F. Anderson; Durland Fish; Erol Fikrig; Fred S. Kantor
Journal of Immunology | 1991
Jonathan Sears; Erol Fikrig; Terry Y. Nakagawa; Kathleen DePonte; Nancy Marcantonio; Fred S. Kantor; Richard A. Flavell
Infection and Immunity | 1993
Linda K. Bockenstedt; Stephen W. Barthold; Kathleen DePonte; Nancy Marcantonio; Fred S. Kantor
American Journal of Tropical Medicine and Hygiene | 2006
Joao H. F. Pedra; Sukanya Narasimhan; Kathleen DePonte; Nancy Marcantonio; Fred S. Kantor; Erol Fikrig