Nandita Ghoshal

Effects of farmyard manure and inorganic fertilizer on the dynamics of soil microbial biomass in a tropical dryland agroecosystem

Changes in the soil microbial biomass following applications of farmyard manure and inorganic fertilizer, alone and in combination, were studied for two annual cycles in a rice-lentil crop sequence grown under rainfed tropical dryland conditions. During the two annual cycles the microbial biomass C range (μg g-1) was 146–241 (x = 204), 191–301 (245), 244–382 (305), and 294–440 (365) in control, fertilizer, manure and manure+fertilizer plots, respectively. The corresponding ranges for microbial biomass N (μg g-1) were 16.5–21.0 (19.5), 20.4–38.2 (26.0), 23.0–34.6 (27.0) and 26.2–42.4 (33.3), and for microbial biomass P (μg g-1) 4.4–8.2 (7.0) 6.0–11.2 (9.6), 11.2–22.0 (17.0), and 10.0–25.4 (18.3). The maximum increase in the microbial biomass, due to these inputs was observed under the manure+fertilizer treatment followed, in decreasing order, by manure alone and fertilizer alone. Within individual crop periods the levels of microbial biomass decreased sharply from the seedling to the flowering stage and then increased slightly with crop maturity. The maximum levels of microbial biomass C and P were observed during the summer fallow. The maximum accumulation of microbial biomass N occurred in the early rainy season, immediately after the soil amendments. Microbial biomass C, N, and P were positively related to each other throughout the annual cycle.

Microbial Biomass Dynamics in a Tropical Agroecosystem： Influence of Herbicide and Soil Amendments

The influences of herbicide alone and in combination with the soil amendments with contrasting resource qualities on dynamics of soil microbial biomass C (MBC), N (MBN), and P (MBP) were studied through two annual cycles in rice-wheat-summer fallow crop sequence in a tropical dryland agroecosystem. The experiment included application of herbicide (butachlor) alone or in combination with various soil amendments having equivalent amount of N in the forms of chemical fertilizer, wheat straw, Sesbania aculeata, and farm yard manure (FYM). Soil microbial biomass showed distinct temporal variations in both crop cycles, decreased from vegetative to grain-forming stage, and then increased to maximum at crop maturity stage. Soil MBC was the highest in herbicide + Sesbania aculeata treatment followed by herbicide + FYM, herbicide + wheat straw, herbicide + chemical fertilizer, and herbicide alone treatments in decreasing order during the rice-growing period. During wheat-growing period and summer fallow, soil MBC attained maximum for herbicide + wheat straw treatment whereas herbicide + FYM, herbicide + Sesbania, and herbicide + chemical fertilizer treatments showed similar levels. The overall trend of soil MBN was similar to those of soil MBC and MBP except that soil MBN was higher in herbicide + chemical fertilizer treatment over the herbicide + wheat straw treatment during rice-growing period. In spite of the addition of equivalent amount of N through exogenous soil amendments in combination with the herbicide, soil microbial biomass responded differentially to the treatments. The resource quality of the amendments had more pronounced impact on the dynamics of soil microbial biomass, which may have implications for long-term sustainability of rainfed agroecosystems in dry tropics.

Variation in soil microbial biomass in the dry tropics: impact of land-use change

The impact of land-use change on soil microbial biomass carbon (C) and nitrogen (N) was studied through two annual cycles involving natural forest, degraded forest, agroecosystem and Jatropha curcas plantation. Soil microbial biomass C and N, soil moisture content and soil temperature were analysed at upper (0–10 cm), middle (10–20 cm) and lower (20–30 cm) soil depths during the rainy, winter and summer seasons. The levels of microbial biomass C and N were highest in the natural forest, followed in decreasing order by Jatropha curcas plantation, degraded forest and the agroecosystem. The highest level of soil microbial biomass C and N was observed during summer, decreasing through winter to the minimum during the rainy season. Soil microbial biomass C and N decreased with increasing soil depth for all land-use types, and for all seasons. Seasonal variation in soil microbial biomass was better correlated with the soil moisture content than with soil temperature. The microbial biomass C/N ratio increased with the soil depth for all land-use types, indicating changes in the microbial community with soil depth. It is concluded that the change in land-use pattern, from natural forest to other ecosystems, results in a considerable decrease in soil microbial biomass C and N. Jatropha plantation may be an alternative for the restoration of degraded lands in the dry tropics.

INFLUENCE OF RESOURCE QUALITY OF ORGANIC INPUTS ON RICE-BARLEY DRYLAND AGRO-ECOSYSTEM: VARIATIONS IN BIOLOGICAL PRODUCTIVITY, GRAIN YIELD AND EFFICIENCY OF NITROGEN USE

A two-year study was undertaken in a tropical dryland agro-ecosystem to evaluate the effect of the application of soil amendments with contrasting chemical natures on crop productivity, grain yield, N-uptake and N-use efficiencies. The treatments involved the addition of equivalent amounts of N (80 kg N ha-1) through chemical fertilizer and three organic inputs at the beginning of the annual cycle: Sesbania aculeata shoots (high quality, C/N 16), wheat straw (low quality, C/N 82) and Sesbania+wheat straw (high and low quality combined, C/N 47), together with a control treatment. Test crops consisted of an annual sequence of rice and barley, sown in the rainy and winter seasons, respectively. Fertilizer and Sesbania inputs resulted in higher total net productivity (TNP) for the rice crop (47 % and 32 % increases over the control, respectively) than the combined (+28 %) and wheat straw treatments (+10 %). During the succeeding barley crop, maximum TNP was recorded in the Sesbania+wheat straw treatment (+52 %), followed by wheat straw (+43 %), fertilizer (+19 %) and Sesbania (+17 %). The TNP and grain yields of both crops added together were higher in Sesbania+wheat straw and fertilizer treatments compared to a single applications of either Sesbania or wheat straw. The Sesbania+wheat straw and fertilizer treatments resulted in more efficient utilization of N compared to the other treatments. Crop roots played a pivotal role in N-recovery from the soil and their N concentrations differed significantly (p < 0.05) due to the application of soil amendments. Across different treatments, crop root biomass was strongly correlated with crop N-uptake (r = 0.81, n = 10, p < 0.05), recovery efficiency (r = 0.81, n = 8, p < 0.05) and agronomic efficiency (r = 0.81, n = 8, p < 0.05). It is suggested that the combined application of high and low quality resources modulated N release, resulting in relatively higher productivity through the annual cropping cycle. Such combined inputs may prove useful in developing low input, environment friendly soil management practices in tropical dryland agro-ecosystems.

Impact of Land-Use Change on Soil Microbial Community Composition and Organic Carbon Content in the Dry Tropics

Abstract Restoration of forests poses a major challenge globally, particularly in the tropics, as the forests in these regions are more vulnerable to land-use change. We studied land-use change from natural forest (NF) to degraded forest (DF), and subsequently to either Jatropha curcas plantation (JP) or agroecosystem (AG), in the dry tropics of Uttar Pradesh, India, with respect to its impacts on soil microbial community composition as indicated by phospholipid fatty acid (PLFA) biomarkers and soil organic carbon (SOC) content. The trend of bacterial PLFAs across all land-use types was in the order: NF > JP > DF> AG. In NF, there was dominance of gram-negative bacterial (G − ) PLFAs over the corresponding gram-positive bacterial (G + ) PLFAs. The levels of G − PLFAs in AG and JP differed significantly from those in DF, whereas those of G + PLFAs were relatively similar in these three land-use types. Fungal PLFAs, however, followed a different trend: NF > JP > DF = AG. Total PLFAs, fungal/bacterial (F/B) PLFA ratio, and SOC content followed trends similar to that of bacterial PLFAs. Across all land-use types, there were strong positive relationships between SOC content and G − , bacterial, fungal, and total microbial PLFAs and F/B PLFA ratio. Compared with bacterial PLFAs, fungal PLFAs appeared to be more responsive to land-use change. The F/B PLFA ratio, fungal PLFAs, and bacterial PLFAs explained 91%, 94%, and 73% of the variability in SOC content, respectively. The higher F/B PLFA ratio in JP favored more soil C storage, leading to faster ecosystem recovery compared to either AG or DF. The F/B PLFA ratio could be used as an early indicator of ecosystem recovery in response to disturbance, particularly in relation to land-use change.