Naoko Dosaka

Lymphomatoid papulosis: ultrastructural immunohistochemical and gene analytical studies

Ultrastrutual, immunohistochemical and gene analytical studies were carried out on a 39‐year‐old patient with lymphomatoid papulosis. Two different cell groups were demonstrated in the papulonodular eruptions: large atypical cells with multiple nuclei that were well stained with anti‐Tac, but not with Leu 3a, and other cells that possessed prominent hyperchromatic nuclei and which stained well with Leu 1 and Leu 3a but not with anti‐Tac. Gene analytical studies using EcoRI, BamHI and HindIII revealed no rearrangement, indicating a non‐clonal T‐cell proliferation unlike malignant T‐cell lymphoma. These results suggest that the present case was benign.

Analysis of Epidermal Growth Factor Receptor Gene in Psoriasis

Epidermal Growth Factor (EGF) plays an important role in cell proliferation. In psoriasis, increased histochemical expression of EGF receptor has been reported in the epidermis. In order to elucidate the mechanism of this increase, we studied the EGF receptor gene organization in psoriasis. DNAs were extracted from white blood cells of 5 patients with psoriasis and 5 normal controls and also from epidermis of 2 psoriatic patients and 1 normal control, and analyzed by Southern blot technique. There were no differences in the structural organization of EGF receptor gene in either white blood cells or epidermis between psoriatic patients and normal controls. These results suggest that the histochemical increase of EGF receptor in the epidermis of psoriatic patients is not due to a structural change in this gene.

An immuno-histochemical review of EGF receptors in tumors of the epidermis.

皮膚有棘細胞癌を初めとする表皮系の腫瘍に対して抗EGFリセプター抗体と抗基底細胞抗体3B4-6 (当教室にて作製したマウスモノクローナル抗体) とKi-67抗体を用いて免疫組織学的に検討した。皮膚有棘細胞癌は, 他の腫瘍と比べてEGFリセプターが特異的に増加しており, その先行病変である熱傷後瘢痕や放射線皮膚炎においても表皮構築の乱れや分裂増殖層の増加は無いにもかかわらず, EGFリセプターが既に増加していた。皮膚有棘細胞癌において, その発症や増殖のメカニズムにEGFリセプターが関与していることが示唆された。

Analysis of epidermal growth factor receptor gene in a case of multiple Bowen's disease.

A 45‐year‐old woman had a triple cancer, multiple Bowens disease, breast cancer, and carcinoma in situ of the cervical portion of her uterus. Development of all three carcinomas was discovered within a period of three years. Southern blotting analysis of the epidermal growth factor receptor (EGFRc) gene of DNA extracted from the individual lesions of multiple Bowens disease revealed no amplification or variant other than one extra band observed in one lesion. DNA extracted from other patients with solitary Bowens disease showed essentially the same result. In contrast to squamous cell carcinoma DNA which sometimes has amplification of this gene, our present case suggests that there is only a small possibility that the EGFRc gene plays a role in the carcinogenesis of Bowens disease.