Naoto Ishijima

The immunohistochemical expression of BCL-2 oncoprotein in colorectal adenocarcinoma

The immunoreactivity forbcl-2 oncoprotein was determined in tumor tissue samples from 40 patients with colorectal adenocarcinoma. Positive immunoreactivity forbcl-2 oncoprotein was seen in 14 of the 40 tumor tissue samples (35%) and was found to be associated with a significantly higher incidence of synchronous liver metastasis (P=0.043); however, there was no correlation between the immunoreactivity forbcl-2 oncoprotein and tumor size, depth of tumor invasion, lymph node metastasis, or clinical stage of the disease. Among 33 patients who had undergone curative resection, disease-free survival did not differ significantly between 10 withbcl-2-positive tumors and 23 withbcl-2-negative tumors (P=0.498). The present study showed that the positive immunohistochemical expression ofbcl-2 oncoprotein was associated with a significantly higher incidence of liver metastasis from colorectal cancer, but it had no effect on the disease-free survival of patients who had undergone curative resection.

Expression of c-myc Oncoprotein p62 in Normal Colon Mucosa and Colorectal Neoplasms: An Immunohistochemical Study

Immunoreactive staining for the c-myc oncoprotein (p62) was examined in 58 colorectal carcinomas, 17 colorectal adenomas, and 64 samples of normal colon mucosa using a mouse monoclonal antibody agains

DNA ploidy, c-myc oncoprotein p62 and v-H-ras oncoprotein p21 in colorectal carcinoma

DNA ploidy and expression of the c-myc oncoprotein p62 and the v-H-ras oncoprotein p21 were examined in 54 colorectal carcinomas. DNA ploidy, determined by DNA flow cytometry, was diploid in 19 samples and aneuploid in 35. Expression of the p62 oncoprotein, determined by immunohistochemical staining, was intensely positive in 18 samples while that of the p21 oncoprotein, also determined by immunohistochemical staining, was positive in 29. There was no correlation between DNA ploidy and expression of the p62 oncoprotein, and DNA ploidy did not correlate with expression of the p21 oncoprotein. There was, however, a close correlation between expression of the p62 oncoprotein and that of the p21 oncoprotein being P<0.01 according to Peasons chisquare test.