Narinobu Inouye

The complete nucleotide sequence of odontoglossum ringspot virus (Cy-1 strain) genomic RNA.

The complete nucleotide sequence of the genomic RNA of odontoglossum ringspot virus Cy‐1 strain (ORSV Cy‐1) was determined using cloned cDNA. This sequence is 6611 nucleotides long containing four open reading frames, which correspond to 126 K, 183 K, 31 K, and 18 K proteins. Its genomic organization is similar to other tobamoviruses, TMV‐V(vulgare), TMV‐L (tomato strain), tobacco mild green mosaic virus (TMGMV) and cucumber green mottle mosaic virus (CGMMV). The 5′ non‐coding regions of ORSV Cy‐1 is 62 nucleotides. The ORFs encoded a 126 K polypeptide and a 183 K read‐through product in which helicase‐sequence and polymerase‐sequence motifs are found. The ORFs encoding the 126 K and 183 K proteins have 61% and 63% identities with those of TMV‐V. The third ORF encoded a 31 K protein homologous to TMV cell‐to‐cell movement protein. It has 63% identities with that of TMV‐V. The fourth ORF encoded an 18 K coat protein. The 5′ non‐coding region, which extends from base 1 to 62 has 2 G residues and a ribosome binding site (AUU). The 3′ non‐coding region, 414 nucleotides in length, is entirely different from that of other tobamoviruses.