Naruhiko Nagao

Serum activity of C1 inactivator in Duchenne-type progressive muscular dystrophy.

We previously found that the level of the immunoreactive activity of C1 inactivator in the plasma Duchenne-type patients with progressive muscular dystrophy was lower than that in normal boys. Therefore, we investigated the level of the C1 inactivator inhibiting activity against C1 esterase in the serum from PMD patients. The mean level of anti-esterase activity of C1 inactivator against C1 esterase in the serum from PMD was 50% lower than that of the control group (P less than 0.05).

Dialysis experiments on urinary immunoreactive human growth hormone by means of a collodion bag

Abstract A convenient method to assay human growth hormone in urine was described. The pH of 20 ml of urine was adjusted to 8.6 with 1 n NaOH, and the sample was centrifuged. To the supernatant was added 0.1 ml of 2% bovine serum albumin, and the sample was concentrated against a borate buffer, pH 8.6, in vacuo, by using a Sartorius membrane filter. The mean recovery ratio of added immunoreactive growth hormone was 91.9%.