Natalija Džinić

Effects of rapid chilling of carcasses and time of deboning on weight loss and technological quality of pork semimembranosus muscle.

The effect of rapid air chilling of carcasses in the first 3 h of chilling at -31°C (then at 2-4°C, till 24 h post-mortem) and the possibility of earlier deboning (8 h post-mortem) after rapid air chilling, compared to conventional air chilling (at 2-4°C, till 24 h post-mortem) on weight loss and technological quality (pH value, tenderness, drip loss, cooking loss and colour - L(∗)a(∗)b(∗) values) of pork M. semimembranosus was investigated. Under the rapid chilling conditions, weight loss was 0.8% at 8 h post-mortem and increased to 1.4% at 24 h post-mortem when weight loss was 2.0% under conventional chilling. Carcasses that were rapid chilled had significantly lower (P<0.001) internal temperature in the deep leg at 4 (25.7°C), 6 (13.0°C), 8 (6.2°C) and 24 h (3.8°C) post-mortem compared to conventional chill treatment (32.7, 24.2, 19.1 and 5.1°C, respectively). Rapid chilling reduced significantly (P<0.05) the rate of pH value decline at 8 h (6.02) post-mortem in M. semimembranosus compared to conventional chill treatment (5.88). Compared to conventional chilling, in M. semimembranosus deboned in different time post-mortem, rapid chilling had a positive significant effect on drip loss (P<0.05, muscles deboned 8 h post-mortem), cooking loss (P<0.001) and incidence of pale colour (L(∗) value). Rapid chilling i.e. rapid chilling and earlier deboning had neither positive nor negative significant effects (P>0.05) on other investigated technological quality parameters of M. semimembranosus (tenderness, a(∗) value and b(∗) value) compared to conventional chilling.

Effect of Starter Culture Addition and Processing Method on Proteolysis and Texture Profile of Traditional Dry-Fermented Sausage Petrovská klobása

Proteolysis and texture profile of Petrovská klobása, made with addition of starter culture, were investigated during the ripening in different conditions (traditional versus industrial). Due to intensive degradation of myofibrillar proteins, significant increases of non-protein nitrogen and free amino acids nitrogen contents were registered in all samples. Similar proteolytic changes were found in both starter and non-starter sausages, but were more pronounced in samples ripened in industrial room. In relation to ripening conditions hardness and chewiness differed significantly, while starter culture had significant effect only on hardness of sausages processed in industrial room.

The Influence of Garlic Powder in Broiler Feed on Carcass and Breast Meat Quality

In this study the carcass and quality (nutritive, technological and sensory) of broiler breast meat were analysed. Hubbard chickens were used for investigations (n=200). There were two groups of samples: control group (C) chickens fed with standard mixture, and experimental group (E) chickens fed with standard feed mixture supplemented with 2% of garlic powder. The trial lasted 42 days. Experimental group had higher (p<0.05) meat yield in chickens breast, but significant effect of garlic addition on nutritive quality of chicken breast meat were not noted. In the breast meat of the experimental group E significantly (P < 0.05) higher protein content and lower (P < 0.05) fat content, compared to control (C), was found. Cholesterol levels in breast meat of the experimental group E (46.1 mg/100g) was significantly lower (P < 0.05) compared to the control group (60.1 mg/100g). The average technological quality of breast meat of groups regarding to pHk, as the parameter and criteria for determining the quality of breast meat corresponds to PSE. The average technological quality of breast meat of control and experimental group according to colouru (L*), as the parameter and criteria for determining the quality of breast meat, corresponds to “normal quality”. According to results of sensory analyzes, roasted breast meat of the experimental group had significantly (P < 0.01) preferable juiciness and tenderness.

Influence of collagen and natural casings on the polycyclic aromatic hydrocarbons in traditional dry fermented sausage (Petrovská klobása) from Serbia

ABSTRACT The aim of this study was to determine the content of polycyclic aromatic hydrocarbons—PAHs (acenaphthylene, anthracene, fluorene, phenanthrene, pyrene, benz[a]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, chrysene, indeno[1,2,3-cd]pyrene, dibenz[a,h]anthracene, and benzo[ghi]perylene)—from Environmental Protection Agency list (United States Environmental Protection Agency) in traditional dry fermented sausage (Petrovská klobása) stuffed in collagen (C) and natural casings (N). Benzo[a]pyrene as well as PAH4 (benz[a]anthracene, benzo[b]fluoranthene, benzo[a]pyrene, and chrysene) were not detected in all examined samples. Results obtained in this study indicated that at the end of drying, as well as at the end of storage period, total content of 13 US-EPA PAH was significantly (P < 0.05) lower in sausages with collagen casing (56.2 and 73.6 μg/kg, respectively) than in sausages with natural casing (137.1 and 206.2 μg/kg, respectively).

The Impact of Replacing Backfat With Microcrystalline Cellulose Gel on Physico-Chemical and Sensory Quality of Frankfurter

The aim of this study was to evaluate the effect of different concentrations of dietary fiber Vitacel MCG 0018 Gel (consisting of microcrystalline cellulose - MCC and two different qualities of carboxymethyl cellulose - CMC) as back fat substitute on the physico-chemical and sensory quality of frankfurter. Five production series were evaluated to examine the back fat replacement: the control group (C) - a sample with just the addition of back fat (100% fat - 0% Vitacel MCG 0018 Gel); 75% fat and 25% gel (E1); 50% fat and 50% gel (E2); 25% fat and 75% gel (E3); 0% fat and 100% gel (E4). In our research, the moisture content increased (59.69% - 71.07%) and the fat content decreased (23.48% - 9.99%) with increasing of back fat replacement by Vitacel MCG 0018 gel. The protein content was higher than 10% (10.18% - 10.35%) and relative content of connective tissue proteins was lower than 20% (6.33 – 17.43) in all examined samples which is in accordance with the criteria of the Serbian Regulation. Sensory evaluation of color, smell and taste and texture and juiciness are less assessed in frankfurters where the fat is replaced by the Vitacel MCG 0018 gel in the proportion of more than half. In general, it can be concluded that the replacement of up to 50% back fat with cellulose fiber gives sausages almost unchanged properties, while the addition of larger fiber amount leads to decrease of sausage quality.

The effect of essential oil from sage (Salvia officinalis L.) herbal dust (food industry by-product) on the microbiological stability of fresh pork sausages

The effect of essential oil obtained from sage (Salvia officinalis L.) herbal dust (a food industry by-product) (SEO), on the pH value, microbiological stability and sensory properties of fresh pork sausages prepared without chemical additives was evaluated during 8 days of aerobic storage at 3±1°C. The addition of SEO significantly (p<0.05) reduced the microbial growth in fresh pork sausages. Moreover, SEO added at a level of 0.05 µL/g had no negative effect on sensory properties of this meat product. Hence, the results of this study showed significant antimicrobial activity of SEO obtained from sage filter tea processing byproducts and the potential for utilising SEO in fresh pork sausages in order to enhance their stability and safety.

Neki parametri nutritivnog kvaliteta mesa svinja rase mangulica i landras

The aim of this study was to provide a comparative analysis of chemical and fatty acid composition, as well as of the connective tissue proteins in pigs of different genotypes, Mangalitsa and Landrace. Both pig genotypes were fed with the same feed of standard composition and quality. At the end of the fattening period, in total 24 pigs of both genotypes were slaughtered. Based on the analysis of the chemical composition we came to the conclusion that the protein content in both genotypes was similar. Moisture and ash content in the Landrace pig genotype differed significantly (P<0.01) compared with genotype of pigs Mangalitsa breed. Statistically significant differences (P<0.01) were established in the fat content, which was 7.95 g/100g, in pigs of Mangalitsa breed and 1.59 g/100g in the Landrace pigs breed. Content of hydroxyproline, non-proteinogenic amino acids, in meat of Landrace was significantly higher (P<0.01) compared to the content in the Mangalitsa breed. The same tendency was observed with regard to the connective tissue protein content as well as with the relative connective tissue protein content. The fatty acid composition of the meat indicated that the most common saturated fatty acid (SFA) in both tested breeds was palmitic fatty acid (C16), whose content was significantly higher in Landrace (P<0.01) compared with its content in Mangalitsa breed. In addition, the share of stearic acid (C18) was significantly higher (P<0.01) in Landrace compared to Mangalitsa pig breed, what significantly contributed to the increase of the SFA share in Landrace compared to Mangalitsa breed. The most common monounsaturated fatty acid in both pig breeds was the oleic fatty acid (C18:1), whose share was significantly higher in Mangalitsa compared to the Landrace breed (P<0.01). Out of the polyunsaturated fatty acids (PUFA), linoleic fatty acid (C18:2) was the most predominant in both pig breeds, with no statistically significant differences (P>0.05). The content of PUFA was not statistically significantly different between the tested breeds, as well as the content of n-3 and n-6 fatty acids, which caused no statistically significant differences in the n-6/n-3 PUFA ratio. Ratio of the unsaturated fatty acids, i.e., of the sum of MUFA and PUFA, and of the saturated fatty acids was significantly higher in Mangalitsa compared to Landrace breed (1.86 versus 1.4), and the same was observed when it comes to the relationship MUFA/SFA (1.51 in Mangalitsa versus 1.08 in Landrace breed) and MUFA/PUFA (4.35 versus 3.38).

Chemical, Color, Texture and Sensory Properties of Čajna Kobasica, a Dry Fermented Sausage

The aim of this study was to conduct a comprehensive examination of quality parameters of dry fermented sausage (Cajna kobasica) from six different producers from the market in Vojvodina. The investigated parameters were chemical composition (moisture, total protein, relative content of connective tissue proteins RCCTP, free fat, ash, NaCl and nitrite content), pH value, aw value, instrumental color parameters (lightness L*, redness a* and yellowness b*), instrumental texture parameters (hardness, springiness, cohesiveness and chewiness) and sensory properties (external appearance and/or condition of the packaging, appearance and composition of cut surface, color and stability of color, odour and taste and texture and/ or juiciness). It could be concluded that out of six examined brands of dry fermented sausage (Cajna kobasica), two brands did not meet the requirements prescribed under the Regulations, because of a higher level of RCCTP. The lowest L* value was recorded in brand 4 (44.33). The brand 4 had the highest hardness and chewiness (22883.33g and 3857.58g, respectively), and the lowest springiness and cohesiveness (0.42 and 0.40, respectively). Parameters related to sensory properties showed significant variability (p < 0.05). Overall sensory quality of tested samples ranged from 3.53 (brand 1) to 4.18 (brand 4).

Quality Standardization of Traditional Dry Fermented Sausages: Case of Petrovská klobása

The European market of dry-cured meat products is characterized by a wide range of traditional fermented sausages, which come from different parts of the continent, primarily from the Mediterranean. Production of these products has lasted for centuries and when the first sausage was produced remains uncertain, because its production dates from the period before written history. Traditional dry-fermented sausages, originating from different countries and regions, are characterized by specific sensory and physicochemical properties affected by the environmental conditions, raw materials, production method, activity and composition of present microflora, biochemical and physicochemical changes occurring during the smoking, fermentation, drying, and ripening process, and specificities of the local population (customs, habits, traditions, religion, etc.). Diversity in appearance, color, taste, smell, and texture of these products can contribute to the preservation of gastronomic heritage and culture, as well as economic development of rural areas by increasing consumption and production volume (Santos et al. 1998; Casaburi et al. 2007; Aquilanti et al. 2007; Talon et al. 2007; Latorre-Moratalla et al. 2008; Roseiro et al. 2008; Settanni and Moschetti 2014).

Biogenic Amines Profile of Serbian Traditional Sausage in Relation to Raw Material and Production Conditions

Petrovská klobása is a traditional dry fermented sausage produced in the Autonomous Province of Vojvodina. Formation of nine biogenic amines was determined in three sausage groups produced from hot deboned (A1) and cold meat (B1, B3) during drying and ripening in traditional room (A1, B1) and in industrial ripening chamber (B3). Dansyl chloride derived amines were analysed by high-performance liquid chromatography with diode-array detector (HPLC-DAD) on Eclipse XDB-C18 column. Histamine, the most important amine from food safety point of view, was not found in any of the analyzed samples. Serotonin and spermidine were also not detected in any samples, while spermin was determined in all analyzed samples. At the end of drying, phenylethylamine was the predominant amine in A1 sausage group (51.6 mg/kg), and tryptamine in B1 (38.1 mg/kg) and B3 (28.7 mg/kg) sausage groups. At the end of ripening, tryptamine was the predominant biogenic amine in all sausage groups (133, 121 and 39.8 mg/kg in A1, B1 and B3 groups, respectively). Total level of biogenic amines in all investigated sausages did not exceed 174 mg/kg at the end of drying and 238 mg/kg at the end of ripening period. Tryptamine and cadaverine may be used as indicators of total biogenic amines for sausages produced in traditional and indusrial conditions from hot deboned and cold meat.