Nathalie Mandonnet

Genetic variability in resistance to gastro-intestinal strongyles during early lactation in Creole goats

The study was undertaken in a Creole goat flock at INRA-Gardel in Guadeloupe, to evaluate the opportunity to use artificial selection as a means of controlling gastro-intestinal infection during early lactation. The flock grazed all year on Digitaria decumbens pastures. Faecal and blood samples were taken from kids at 11 months of age and from does at kidding before drenching (week 0) and at weeks 4 and 6 after kidding. Faecal egg counts (FEC) were estimated using a modified McMaster method. Blood samples were used to determine packed cell volume (PCV) and eosinophil concentrations (EOS) values. Haemonchus contortus , Trichostrongylus colubriformis and Oesophagostomum columbianum were the main strongyle species identified in faecal cultures. The data came from 1092 litters obtained from 688 does sired by 142 bucks and 413 dams. Variance and covariance components for genetic and residual effects were estimated with multivariate animal models using the restricted maximum likelihood VCE package. Repeatability and overall heritability for FEC during the post-partum period were 0·17 and 0·10±0·02. The genetic correlations between FEC and PCV were −0·56±0·11 at 4 weeks after kidding and −0·79±0·13 at 6 weeks after kidding. The genetic correlations between FEC and EOS were 0·37±0·15 at 4 weeks after kidding and 0·68±0·17 at 6 weeks after kidding. Hence, does that contributed least to pasture contamination during the postpartum period also had low EOS and high PCV breeding values. The genetic correlations between FEC measured at 11 months of age and FEC during periparturient period ranged from 0·57±0·12 to 0·76±0·16. Therefore, breeding goats for increased resistance during the post-weaning period will lead to a less marked and less persistent rise in doe FEC during early lactation. The epidemiological implications of this selection have to be quantified in terms of lower pasture contamination, lower kid parasitism, and higher milk production of does.

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus.

The objective of this study was to evaluate the relationship of parasite-specific serum antibodies with the resistance status of Creole kids. The average breeding values on egg output predicted in a context of natural infection at 11 months of age were distant of 1.07 genetic standard deviation between resistant and susceptible animals. After drenching the animals were maintained worm-free during 1 month until experimental infection with 10,000 Haemonchus contortus infective larvae (L3). Enzyme-linked immunosorbent assay was carried out in serum samples to determine the level of IgG, IgA and IgE anti-H. contortus L3 crude extracts and adult excretion/secretion products (ESP). Parasitological and blood immunological parameters were measured on the 2 extreme groups. Despite the absence of any typical signs of haemonchosis, susceptible kids had more than 11 times higher faecal egg counts (FEC) at 35 days post-infection (d.p.i.) than resistant kids had. Levels of immunoglobulin against H. contortus L3 and ESP increased significantly after infection in both groups. However, no difference in the host immune response mediated by immunoglobulin against H. contortus was evidenced between groups. This finding suggests that, in goats previously infected by H. contortus, a degree of protection occurred and the phenotypic and genetic segregation in resistant and susceptible animals were not related to the humoral immune response. The correlation coefficients between FEC and IgE anti-ESP (r=0.593; P<0.05 was significant in both resistant and susceptible animals. Such correlation suggesting a hypersensitivity reaction dependent on worm prolificacy has never been described. This result needs further studies to understand the mechanisms underlying this observation.

Growth performance, carcass quality, and noncarcass components of indigenous Caribbean goats under varying nutritional densities

Studies were conducted to determine the effects of feeding regimens on growth and carcass quality of the Creole goat, a genotype indigenous to the Caribbean. Forty kids weighing 9.0 +/- 1.2 kg of BW were reared indoors after weaning. Four supplement amounts were compared (10 kids per treatment): the G0 group received the basal diet (tropical forage, 8.8 MJ of ME and 108 g of CP/kg of DM) without concentrate, whereas the G100, G200, and G300 groups were offered 130, 230, and 310 g/d of concentrate (13.6 MJ of ME and 209 g of CP/kg of DM), respectively, in addition to the basal diet. The kids were slaughtered according to the standard procedure at 22 to 24 kg of BW for assessment of carcass traits and meat quality. Total DMI increased significantly, from 51 to 78 g/kg of BW(0.75), for G0 to G300 kids, whereas their ADG doubled from 42 to 84 g/d (P < 0.01; P < 0.01, respectively). The G:F values reached 125 to 130 for the G200 and G300 diets and were satisfactory compared with literature values. The carcass weight and dressing percentage (P < 0.01) increased from group G0 to G300, from 9 to 13 kg and from 42 to 51%, respectively. The proportions of the different cuts (related to the carcass weight) did not vary by diet. The conformation score increased significantly (P < 0.05) among the 4 groups from an average score of 3.2 to 4.0 (score/5). There was a significant effect (P < 0.01) of supplement amount on the accumulation of internal fat tissues: the kidney fat weight increased from 113 to 253 g from the G0 to the G300 group. Regardless of the feeding level and amount of internal fat, the carcasses had an acceptable fat cover score, which remained less than 2.6 (score/5). A significant effect was not observed for the ultimate pH and the main color variables of the meat. The cooking loss and the shoulder DM content varied (P < 0.05) with the supplement amount. By increasing the nutritional density of the diet, it was possible to obtain well-conformed and heavy carcasses, with no excessive fattening. Indigenous Creole goats have potential as meat animals when fed to gain more than 80 g/d. The optimal supplement supply with good-quality grass would be approximately 3.69 MJ of ME/d in our conditions. Further studies are required on meat sensory parameters and fatty acid profiles.

A short-term divergent selection for resistance to Teladorsagia circumcincta in Romanov sheep using natural or artificial challenge

This experiment was conducted to assess the efficiency of selection on the basis of response to artificial challenges in order to breed sheep resistant to natural infection. A short-term divergent selection process was designed to estimate the genetic parameters of these two traits. Two flocks, including 100 Romanov ram lambs each, were challenged in 1990 when they were 6 months old. One flock received three artificial infections with 20 000 third-stage Teladorsagia circumcincta larvae, at intervals of 7 weeks. Faecal egg counts (FEC) were performed on Days 22, 25 and 28 post infection (p.i.) and the animals were drenched on Day 28 p.i. The other flock was grazed for 5 months on a pasture contaminated with the same species. Faecal samples were taken from the lambs at similar ages. About 5 rams with the lowest FEC and 5 with the highest FEC were selected in each flock and mated with unselected ewes. Their offspring (200 animals) were challenged in 1992, half in the same way as their sires, and the other half by the other method. Because of a drought in the summer of 1990, it was necessary to repeat part of the experiment, and in 1992 the 5 and 8 rams with the lowest and highest FEC, respectively, were selected from the offspring challenged on the pasture in 1992 and were mated with unselected ewes. Their progeny (about 80 animals) were challenged in 1994, half by natural infection, half by artificial infection. The mean FEC of the flock increased from the first to the third artificial infection. The natural infection was highly variable in different years, reflecting the difficulty of assessing resistance using this mode of challenge. Genetic parameters were estimated using animal models and REML solutions. The repeatabilities of the FEC following artificial and natural infection were 0.49 and 0.70 respectively within a period of one week, and 0.22 and 0.41 respectively for periods separated by intervals of 7 weeks; the heritabilities of the single egg count were 0.22 and 0.38 respectively. The genetic correlation was 0.87: the FEC recorded under natural or artificial infection appear to depend on the same genetic potential.

Effect of dietary supplementation on resistance to experimental infection with Haemonchus contortus in Creole kids

The aim of the present study was to test the effect of dietary supplementation on resistance to experimental infection with Haemonchus contortus in Creole kids. One trial with three replicates involved a total of 154 female kids that were chosen from three successive cohorts of the Creole flock of INRA-Gardel in 2007. The kids were placed into four treatments according to the amount of concentrate they received: G0 (no concentrate and a quality Dichantium spp. hay ad libitum, HAY), G1 (HAY+100g commercial concentrate d(-1)), G2 (HAY+200 g commercial concentrate d(-1)), G3 (HAY+300 g commercial concentrate d(-1)). The G0-G3 groups were infected with a single dose of 10,000 H. contortus third stage larvae (L(3)) at Day 0 (D0). Each infected group was comprised of one half resistant and one half susceptible genetically indexed kids. The average breeding values on egg excretion at 11 months of age were distant of 0.70, 0.65, 0.61 and 0.61 genetic standard deviations in G0, G1, G2 and G3, respectively. The faecal egg count (FEC), packed cell volume (PCV), eosinophilia (EOSI) and dry matter intake (DMI) indices were monitored weekly until 42 days post-infection. Enzyme-linked immunosorbent assay was carried out on serum samples to determine the level of IgA anti-H. contortus L(3) crude extracts and adult excretion/secretion products (ESP). The 10,000 L(3) dose received by the kids induced a severe infection: 8000 eggs per gram at the FEC peak, a PCV less than 15% and mortality. Interestingly, the supplemented animals in G3 showed a higher level of EOSI but a lower level of IgA anti-L3 and IgA anti-ESP than non-supplemented animals (G0). Resistant and susceptible kids had significantly different FEC variations within the groups. Susceptible kids had a 1.6 times higher egg output than resistant kids in G0. This difference was not found in the supplemented groups. The results of this study showed that supplementary feeding improved resistance of Creole kids to H. contortus experimental infection.

Fifteen years later, anthelmintic resistances have dramatically spread over goat farms in Guadeloupe.

Faecal egg count reduction tests (FECRTs) were performed on 21 goat farms in Guadeloupe (FWI). Anthelmintic resistance (AR) to netobimin (benzimidazole) was found in all 15 herds in which it was tested. AR to ivermectin (avermectin) and levamisole (imidazothiazole) were also very largely spread (14 out of 17 farms and 7 out of 9 farms, respectively). AR to the final moxidectin (milbemycin) released was already present in 2 out of 9 farms in which it was tested. Haemonchus was the dominant genus of gastrointestinal nematodes and was more frequently found to be resistant to netobimin, ivermectin and moxidectin than Trichostrongylus, the latter appeared to be more often resistant to levamisole. A first survey 15 years ago revealed only AR to benzimidazoles and one suspected case of AR to ivermectin.

Intake and digestibility of naive kids differing in genetic resistance and experimentally parasitized (indoors) with Haemonchus contortus in two successive challenges.

We evaluated the effects of infection with Haemonchus contortus on feed intake, digestibility, fecal egg count, circulating eosinophils, and packed cell volume in Creole kids differing in genetic resistance (susceptible, S; resistant, R) to gastrointestinal parasitism and maintained on a similar level of nutrition. The experiment was carried out during 2 periods of 6 wk each differing in immunity development stage. In the first period (acquisition of immunity; period I), 22 naïve male kids (23.4 +/- 0.65 kg of BW) were housed in individual boxes and fed a hay-based diet, and a primary infection was induced. In the second period (expression of immunity; period II), 15 of the initial 22 kids (28.4 +/- 0.77 kg of BW) were submitted to a secondary infection. Housing and management were uniform throughout the experiment. For each period, measurements of intake and digestibility were made at 0, 2, and 4 wk postinfection (WPI) with a single dose of 10,000 infective larvae (L(3)). The DMI and total-tract DM, OM, CP, NDF, and ADF digestibilities were determined using the total feces collection and ad libitum forage supply method. Fecal and blood samples were collected weekly to measure fecal egg count, circulating eosinophils, and packed cell volume. Infection with Haemonchus contortus decreased feed intake during period I. The absence of anorexia in period II was probably due to the acquired immunity of kids. The DMI was affected (P = 0.05) by genetic predisposition to resistance (626 vs. 583 +/- 26 g/d, for R vs. S) and WPI, being greatest in the second WPI (693 vs. 614 and 657 g/d, for WPI-2 vs. WPI-0 and WPI-4, respectively). The latter was related to worm establishment phase and was linked to the lower total tract digestibilities at this point. Digestibilities were least at WPI-2. The fecal egg counts were greater (P < 0.001) in period I than II, and differences between S and R were evident after the fifth WPI in period II. Circulating eosinophils were greater (P < 0.001) in S vs. R. The results suggest that effects of these parasites on intake and digestibility are influenced by the individual genetic resistance and the immunological stage, and the strongest impact occurs between the second and the third WPI, a period during which the immune response is more pronounced, probably due to parasite maturation.

Genetic analysis of the potential role of IgA and IgE responses against Haemonchus contortus in parasite resistance of Creole goats.

Small ruminants are affected by gastrointestinal nematode (GIN) infection. A promising alternative strategy for control of GIN infection is to increase the level of resistance in the population by taking advantage of the hosts immune response. Immunoglobulin A (IgA) and E (IgE) are known to be involved in immune response to GIN. The aim of this study was thus to investigate genetic parameters of IgA and IgE responses against Haemonchus contortus in Creole kids naturally challenged at pasture and to determine the relationship with other resistance criteria such as faecal egg counts, packed-cell volume, eosinophil counts and bodyweight. Variance and covariance components for genetic and residuals effects for each trait were estimated on 3862 males at 11 months of age. Heritability estimates for IgA and IgE ranged between 0.15 and 0.57. Strong positive genetic correlations were observed between either IgE or IgA responses against L3 and adult excretory/secretory products (ESP) antigens of H. contortus, suggesting that the humoral immune response is not specific to the life cycle stage of the parasite suggesting that there is substantial cross recognition between the different parasite antigens. Heritability estimates for faecal egg count (FEC), packed-cell volume (PCV) and bodyweight (BW) were in accordance with previous results in Creole kids. Blood eosinophil counts were found moderately heritable and negatively correlated with FEC, suggesting that this cell population plays a role in resistance to nematode parasite infection in Creole goats. IgA response was positively correlated to FEC, in contrast with the negative correlation between IgE against L3 of H. contortus and FEC. In Creole goats, IgA response against L3 or ESP of H. contortus would rather be associated with the worm burden than an immune protective response. The immune response involving activity of IgE against L3 of H. contortus may be one important pathway for development of resistance to gastrointestinal nematode infections in Creole goats.

Economic values of body weight, reproduction and parasite resistance traits for a Creole goat breeding goal

A specific breeding goal definition was developed for Creole goats in Guadeloupe. This local breed is used for meat production. To ensure a balanced selection outcome, the breeding objective included two production traits, live weight (BW11) and dressing percentage (DP) at 11 months (the mating or selling age), one reproduction trait, fertility (FER), and two traits to assess animal response to parasite infection: packed cell volume (PCV), a resilience trait, and faecal worm eggs count (FEC), a resistance trait. A deterministic bio-economic model was developed to calculate the economic values based on the description of the profit of a Guadeloupean goat farm. The farm income came from the sale of animals for meat or as reproducers. The main costs were feeding and treatments against gastro-intestinal parasites. The economic values were 7.69€ per kg for BW11, 1.38€ per % for FER, 3.53€ per % for DP and 3 × 10(-4)€ per % for PCV. The economic value for FEC was derived by comparing the expected profit and average FEC in a normal situation and in an extreme situation where parasites had developed resistance to anthelmintics. This method yielded a maximum weighting for FEC, which was -18.85€ per log(eggs per gram). Alternative scenarios were tested to assess the robustness of the economic values to variations in the economic and environmental context. The economic values of PCV and DP were the most stable. Issues involved in paving the way for selective breeding on resistance or resilience to parasites are discussed.

Direct genetic and maternal effects affecting litter size, birth weight and pre-weaning losses in Creole goats of Guadeloupe

The litter size (LS), or its equivalent the number of kids born in the litter (NB), of Creole goats in Guadeloupe was studied by two procedures. The first approach considered LS as a single trait and as a characteristic of the mother of the litter, and was studied by an univariate animal model (UAM). The second procedure treated NB, the individual birth weight (BW) and loss of kids (LK) as traits belonging to the animals born in the litter and their dam, in a multitrait animal model (MAM). The heritability for genetic direct effect ( h 2 a ) for LS estimated by UAM (0·14) was 40% lower than the corresponding value for NB estimated by MAM. The most appropriate of the 6 MAMs tested estimated heritabilities ( h 2 a ) of 0·24, 0·22 and 0·17 for NB, BW and LK, respectively, while maternal effects ( h 2 m ) were 0·20, 0·24 and 0·09 for the same characters. The genetic correlations between direct and maternal effects ( r am ) were negative –0·611 and –0·725 for NB and LK, respectively, and not significantly different from zero for BW. This study explored the possibility of using the information on NB, BW and LK recorded in each animal born in the litter in order to analyse the genetic variability of these traits.