Nazan Deniz Yön

The effects of permethrin on rat ovarian tissue morphology

All organisms are exposed to chemical agents during their lifetime. One of these agents is a pesticide that is used as fly killer. In this study we investigated the effects of permethrin on rat ovaries using light and electron microscopy. We used 24 Wistar albino female rats and divided them into 3 groups. Dosages 20 and 40 mg/kg/day permethrin were administered by gavage for 14 days. Normal saline was given to control rats. After treatment, ovarian tissues were collected and prepared for light and electron microscopy evaluation. Negative effects of permethrin were detected on follicular and corpus luteum cell morphology in a dose dependent manner when compared with the control group. Picnotic cellular appearance and condensed chromatin were detected as evidence of apoptotic cell death. Furthermore, degenerative changes were seen in the ultrastructure of mitochondria and endoplasmic reticulum. Thus, these findings suggested that permethrin caused degenerative effects on ovarian morphology in a dose dependent manner.

The effects of titanium dioxide nanoparticles on ultrastructure of zebrafish testis (Danio rerio)

INTRODUCTION Nanotechnology investigates materials at nanoscale level (0.1-100nm in diameter). There are many commercially nanoproducts such as silver, silicon, titanium, zinc, and gold. They are used in a variety of applications and released to the environment. Titanium dioxide (TiO2) is one of the most commonly used nanoparticles (NP). In this study, the ultrastructural effects of TiO2-NP on zebrafish testis tissue were evaluated. MATERIAL AND METHOD Zebrafish were divided into four groups (N=60) as one control and 3 experimental groups (1mg/L, 2mg/L and 4mg/L TiO2). Testis tissues were dissected after 5days of the exposure. Tissues were fixed with 2.5% glutaraldehyde at 4°C. After routine electron microscopy tissue processing, the testis were embedded in epon resin. Ultrathin sections were counterstained with 1% uranyl acetate and lead citrate and examined using a transmission electron microscope. RESULTS Mitochodrial degeneration with swelling and cristae loss were detected in Sertoli cells and spermatogonial cells of TiO2-NP treated groups in a dose-dependent manner. Moreover, autophagic vacuole accumulation were seen in Sertoli cell cytoplasms of the experimental groups. Necrosis was also detected in the 4mg TiO2-NP-treated group. CONCLUSION TiO2-NP has been used in crop production, food additives, medicine, toothpastes, sunscreens, cosmetics, and in waste water treatment, which contaminated the environment. Our findings showed TiO2-NP-induced autophagy and necrosis at higher doses in Sertoli cells, which consequently negatively affected spermatogenic cells and testicular morphology of Zebrafish. It is important to give much more attention to the use of this NP to minimise the possible effects on nature and organisms.

Identification of Primordial Germ Cells: Cytological, Histological and Immunohistochemical Aspects

Primordial germ cells (PGCs) constitute an embryonic cell type that migrate to gonadal precursors and form the gametes. In many animals, PGCs are set apart from somatic cells early during embryogenesis. These cells migrate to gonadal precursors and then constitute gonads so they are useful models for cell motility studies. They have a highlighted importance for development and reproduction studies. Primordial germ cells have morphological differences from the somatic cells. Structure of these cells can be detected with light and electron microscopy in early development stages. This review describes the morphological, histological, molecular and ultrastructural features of primordial germ cells in different animals and gives an overview for simplified identification.