Nazmi Yüksek

Determination of adenosine deaminase activity in cattle naturally infected with Theileria annulata

The purpose of this study was to determine serum ADA activity in cattle naturally infected with Theileria annulata. In this study, a total of 37 cross-bred cattle which 27 of it showing clinical signs of theileriosis constituted infected group and 10 healthy cattle as control group were used as animal materials. Infected group divided into three groups according to their PCV values. Cattle with PCV ≥ 25 were put on group I (n = 9), those with PCV 13–24 were put on group II (n = 11) and those with PCV ≤ 12 were put on group III (n = 7). Microscopical diagnosis of the disease was also made. Hematological parameters, serum enzyme activities (ADA, AST, ALT and ALP) were determined in all cattle. Hematological results revealed that significant progressive decreases in HGB, PLT, PBML counts and ratios from group I onwards to group III, whereas the WBC, PBPL counts and ratios showed an increase from group I onwards to group III. The serum ADA, AST, ALT and ALP activity increased significantly in all infected groups compared to control group. However, these parameters were also observed to decrease progressively from group I to group III. Furthermore, the highest increase in enzyme activities observed in the infected group I. But, these enzyme’s activities started to decrease in infected group II and III in parallel with PBML and PLT counts. Eventhough, this decrease did not reach to the values obtained from control group. On the contrary, PBPL counts and ratios increased in infected group II and III in contrast to decrease in PCV. As a result, increased serum ADA activity in tropical theileriosis may reflect the involvement of the cellular immune responses.

Stomach Impaction in Ostriches (Struthio camelus): Blood Chemistry, Hematology, and Treatment

SUMMARY. This study involved 14 ostriches of both sexes between 3 and 24 mo of age. Some hematologic and biochemical parameters were studied in animals with stomach impaction. Clinical examination of the birds revealed anorexia, emaciation, decreased defecation, listlessness, separation from the flock, and recumbence. The total number of leukocytes (10.4 × 103 mm3), the concentrations of serum glucose (166 mg/dl), and total protein (2.4 g/dl) decreased; serum creatine phosphokinase (1240 U/L) and alkaline phosphatase (598 U/L) increased; whereas no changes were found in the concentrations of calcium, phosphorus, magnesium, zinc, and copper. Medical treatment was initiated in 13 animals; six of them recovered and seven birds died. Necropsy revealed edema, erosions, and hemorrhagic ulcers in the stomach of the dead birds. Foreign bodies such as sand; stone; pieces of wood, glass, and plastic; and metallic objects were encountered in the stomach at the necropsy. In one bird, metallic foreign body was diagnosed and the bird was referred to surgery. Under stress factors, ostriches tend to eat foreign material; therefore, adequate housing, nutrition, and care are crucial in prevention of stomach impaction.

Evaluation of total antioxidant, total calcium, selenium, insulin, free triiodothyronine and free thyroxine levels in cows with clinical and subclinical ketosis

Absract: The purpose of this study was to evaluate serum albumin, glucose, calcium (Ca), cobalt (Co), copper (Cu), iron (Fe), magnesium (Mg), and phosphorus (P) in cows with naturally infected with Theileria annulata. The material of this study was a total of 15 cattle with different ages, breeds and genders diagnosed as theileriosis according to clinical and microscopical examination. According to statistical analyses of biochemical parameters; serum glucose, Ca, Co, Cu, Fe, Mg, and P concentrations were detected significantly low in cattle with theileriosis (P<0.001). In conclusion; mineral substance levels were altered in cattle naturally infected with Theileria annulata. Additionally to the classical treatment of theileriosis, administering mineral supplements including Ca, Co, Fe, Cu, Mg, and P to the animals is thought to be more useful in treatment.

Genetic Structure and Variation of Van Cats

To determine the genetic structure and variation of Van cats and some other cats, seven enzyme loci were examined using horizontal starch gel electrophoresis. ME bands were observed for the first time in cats. For the enzyme loci CA1, SOD, GPI, and GOT, neither the individual Van cats nor the specimens of other cat species exhibited any variation. These enzymes presented identical bands, all of which were homozygous. With respect to the PGD, ME, and ESD loci, however, genetic variation was observed in all of the cats. Hence, three of the seven gene–enzyme systems (43%) were polymorphic with two alleles, contributing to an estimate of average heterozygosity of 0.33–0.49 for the Van cats. PGD was the most discriminatory among the three polymorphic loci. The phylogenetic tree indicated that the Van, Persian, Turkish Angora, and Turkish Tekir cats are distinct from Siamese and Bombay cats.