Nebojsa Simic

Composition and Antibacterial Activity of Achillea asplenifolia Essential Oil

Abstract The oil of a wild growing population of Achillea asplenifolia Vent, was studied for yield, composition and antibacterial activity. The major components in the oil were β-caryophyllene (17.6%), germacrene D (15.6%) and chamazulene (13.3%). In an antibacterial diffusion assay, the oil showed activity against all tested Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) and Gram-positive Staphylococcus aureus.

Composition and Antibacterial Activity of Achillea chrysocoma Essential Oil

Abstract Essential oil of Achillea chrysocoma Friv. was investigated for composition and antibacterial activity. Twenty-eight identified compounds constituted 91.5% of the oil. The main compounds of the oil were borneol (10.1%), terpinen-4-ol (9.2%), cis-p-menth-2-en-1-ol (8.2%) and trans-p-menth-2-en-1-ol (7.9%). Antibacterial tests showed pronounced activity of the oil against Gram-negative bacteria Klebsiella pneumoniae and Pseudomonas aeruginosa.

Essential Oil of Pinus heldreichii, Needles

ABSTRACT Needle oils of Pinus heldreichii Christ, produced from five different growth stages at two different locations in Serbia were examined for yield and composition during 1994. Although only ten components were identified in the oil, the main constituents were limonene (20.26–25.15%), germacrene D (42.6445.42%) and β-caryophyllene (10.58–13.32%). The richness of the oil in germacrene D has chemotaxonomic difference implications.

Volatile constituents of Achillea serbica Nym.

The volatile constituents of Achillea serbica Nym. (Asteraceae), an endemic plant of the Balkan Peninsula, has been analysed using GC and GC–MS. Fifteen identified components constituted 95.7% of the oil. The major fractions belonged to β-sabinyl acetate and camphor amounting to 39.9% and 33.9%, respectively, of the total oil content. Copyright

Biological control of Botrytis cinerea and plant growth promotion potential by Penicillium citrinum in chickpea(Cicer arietinum L.)

A total of 48 fungi were characterised for their antagonistic potential against Botrytis cinerea causing Botrytis Gray Mold (BGM) disease in chickpea by dual culture and metabolite production assays. The culture filtrate of the most promising isolate, VFI-51, was purified by various chromatographic techniques and identified as ‘citrinin’ by nuclear magnetic resonance and mass spectrometry studies. The efficacy of citrinin was demonstrated to control BGM in chickpea under greenhouse conditions. The sequences of 18S rDNA gene of the VFI-51 matched with Penicillium citrinum in BLAST analysis. The VFI-51 produced siderophore, hydrocyanic acid, indole-3-acetic acid, lipase, protease and β-1,3-glucanase; grew well in NaCl (up to 15%), at pH between 7 and 11 and temperatures between 20°C and 40°C; and was compatible with fungicides bavistin and thiram. Under greenhouse and field conditions, VFI-51 significantly enhanced the nodule number, nodule weight, root and shoot weight and stover and grain yield over the un-inoculated control. In the rhizosphere, VFI-51 also significantly enhanced total N, available P and OC over the un-inoculated control. Scanning electron microscopy analysis revealed that VFI-51 colonised on the roots of chickpea. This study concluded that VFI-51 has the potential for biocontrol of BGM and plant growth promotion in chickpea.

1H and 13C NMR data of methyl tetra-O-benzoyl-D-pyranosides in acetone-d6

Complete assignments of 1H‐ and 13C‐NMR resonances of five methyl tetra‐O‐benzoyl‐D‐pyranosides based on 1H, 13C, 2D DQF–COSY, HMQC, HMBC and HSQC–TOCSY experiments have been performed. Copyright

Synthesis and Structure Elucidation of Benzoylated Deoxyfluoropyranosides

Benzoylated deoxyfluoropyranosides have been synthesized, starting with protected, unprotected, or fluorinated precursors. Fluorination of eight derivatives was compared using DAST and Deoxo-Fluor as reagents. Deoxo-Fluor was found to be especially useful in the fluorination of methyl 2,3,4-O-tribenzoyl α-D-mannopyranoside and β-D-glucopyranoside, resulting in better yields and avoiding the 1,6-methoxy migration experienced with DAST for one derivative. The two reagents gave comparable yields in the fluorination of other methyl pyranosides, confirming Deoxo-Fluor as a safer alternative to DAST. Methyl α-D-mannopyranoside underwent fluorination to yield the 4,6-difluorotalopyranoside and the corresponding cyclic sulfite. The NMR spectroscopic properties of 11 benzoyl deoxy-fluoropyranosides are reported.

1H,13C and 19F NMR data of N-substituted 6-(4-methoxyphenyl)-7H-pyrrolo[2,3-d]pyrimidin-4-amines in DMSO-d6

Chemical shift assignment of seven N‐substituted 6‐(4‐methoxyphenyl)‐7H‐pyrrolo[2, 3‐d]pyrimidin‐4‐amines, six of which are fluorinated, have been performed based on 1H, 13C, 19F, and 2D COSY, HMBC and HSQC experiments. Copyright

Essential Oil of Achillea coarctata

Abstract Essential oil of Achillea coarctata Poir. was investigated for yield and composition. Sixty identified compounds in the oil constituted 79-3% of the oil. The main compounds of the oil were: caryophyllene oxide (9.0%), 1,8-cineole (8.5%) and trans-linalool oxide (7.2%).

A novel compound isolated from Sclerochloa dura has anti-inflammatory effects

The activation of PLA 2 by means of pro-inflammatory cytokines results in the subsequent release of arachidonic acid (AA) and generates eicosanoids, which further propagate inflammation. By 6the cyclooxygenases (COX1/2) responsible for the enzymatic conversion of AA to eicosanoids, the non-steroidal anti-inflammatory drugs are effective in relieving the pain and discomfort of inflammation. By using AA release assay as a guide for biological and anti-inflammatory activity, novel compound 1- O -(3- O -linolenoyl-6-deoxy-6-sulfo- α -D-glucopyranosyl)-glycerol (1) together with five known compounds isovitexin, byzantionoside B, tricin 4 ’ - O -(erythro- β -guaiacylglyceryl) ether 7- O - β -glucopyranoside, 1- O -feruloyl glycerol and tricin 7-glucoside were isolated from the methanol extract of the aerial parts of Sclerochloa dura using LC techniques (Sephadex LH-20 column chromatography, preparative HPLC and semi-preparative HPLC). All isolated compounds were identified using spectroscopic NMR spectroscopy and MS spectrometry. Novel compound (1) was found to be an effective inhibitor of AA release with an IC 50 value of 0.09 ± 0.03 mg mL -1 .