Necmi Özdemir

Comparison of Serum Malondialdehyde Levels Determined by Two Different Methods in Patients With COPD: HPLC or TBARS Methods

Thus, the determination of the oxidative stress gradually gets more important during the course of chronic diseases. The results of recent studies have indicated that during the exacerbation of COPD, antioxidant capacity is lower and the levels of lipid peroxidation products are higher than those in age-matched healthy subjects. 2,5,6 These lipid peroxidation products easily create reactive carbon compounds. The most important one is malondialdehyde (MDA). The determination of the level of MDA is the most practical and confidant method used for detecting and screening oxidative stress usually. 7 Malondialdehyde levels may serve as a marker of prognosis and of the success of treatment of the exacerbation of COPD. 2,5,6 Absolute level of free MDA in serum may be also determined with HPLC as a new method without using any reactive component 8 while total MDA level in serum is commonly determined by the TBARS process, which is currently used as a spectrophotometric method. Furthermore, in this method, it is stated that TBA forms complexes with MDA and the other many subtrates such as metal ions and