Neelamegam Rameshkumar

Bacillus tequilensis A Novel Thermotolerant Strain for Effective Bioremediation of Melanoidin Pigment in Its Natural Environment

The present study was aimed to isolate and characterize a novel thermo tolerant melanoidin degrading bacteria from its natural environment. A total number of 35 bacterial strains were isolated among them 2 strains labelled A and H exhibited a maximum of 10% ethanol tolerance and were able to grow at 60 °C. In which, a novel strain A exhibited maximum decolourization 72%, BOD, COD and was able to grow at higher temperature in comparison with other strains. Further, to confirm the identity, it was subjected to morphological, biochemical and molecular characterization. Based on the analysis the identity of the strain A was confirmed as Bacillus tequilensis. The production of MnP and laccase enzymes was observed and decolourization efficiency was analysed through FTIR, the results revealed that most of the predominant compounds present in the control sample were degraded. The reported novel thermo tolerant bacterium Bacillus tequilensis was able to decolorize the melanoidin pigment of distillery effluent more efficiently even at higher temperature than the bacterium reported earlier.