Nell Stein

In vitro cultivation of human renal cell cancer

SummaryTwo cell lines derived from primary human renal-cell cancers (RCC) have been established and characterized. Cell line 786-O has been in culture for longer than 1 year and has been subcultured more than 50 times. It has a doubling time of 45 hr and a hypertriploid karyotype and possesses a Y chromosome. Cell line 769-P also has been in culture for longer than 1 year. It has been subcultured 50 times and has a doubling time of 35 hr and a hypodiploid karyotype. Cells from both lines are epithelial, and they produce tumors in the cheek pouches of immunosuppressed hamsters. Neither cell line is contaminated withMycoplasma. Cells of the two lines can be distinguished from HeLa cells both by their karyotypes and by the mobility patterns of their isoenzymes of glucose-6-phosphate dehydrogenase.

In vitro cultivation of human renal cell cancer. I. Establishment of cells in culture.

SummaryA technique for initiating and propagating epithelial cell cultures of human renal cell cancer and adjacent nontumor kidney is described. Seventy-five percent of the tumors and 79% of the adjacent kidney specimens cultured with this method have shown initial outgrowth and have been subcultured at least once. Two renal cell cancer cultures initiated by this method have now been in continuous culture more than 6 months, have been subcultured 27 and 19 times, and now appear to be stable lines.The ability to establish long-term in vitro cultures of human renal cell cancers will facilitate studies concerning the immunoreactivity, cholesterol metabolism, the isolation of renal-cell-cancer-specific antigens, and in vitro chemotherapy testing and will further our understanding of the basic biology of human renal cell cancer.

Isolation of RNA Virus from Papillary Tumors of the Human Renal Pelvis

Virus particles were detected by electron microscopy in three papillary cancers of the human renal pelvis. Similar particles were seen in cells from all three tumors in primary culture. An RNA virus was isolated from these tumors. The serum of tumor patients contains neutralizing antibodies against virus isolated from two of the tumors. Initial studies suggest that this agent is not a known human RNA virus.

Technique for cultivation of transitional epithelium from mammalian urinary bladder

SummaryA technique for initiating cultures of epithelial (urothelial) cells from mammalian urinary bladder has been described. Urothelial cells obtained by this method have been used to support replication of viruses and as controls for immunological, biochemical, chromosome, and electron microscopy studies. Both light and electron microscopic studies of cultured cells suggest that they are epithelial and not a mixed culture.

A GASTRIC FACTOR PROMOTING IRON ABSORPTION

Abstract Gastric juice from patients with irondeficiency anaemia or haemochromatosis contains a factor which promotes the absorption of iron-59 (as ferrous citrate) mildly in normal rats and strikingly in gastrectomised anaemic rats.

Effect of Ethanol on Absorption of Iron in Rats.

Conclusions Long or short term alcohol administration appeared to have no effect in normal healthy rats on either total body uptake or the isolated intestinal segment uptake of Fe59 in its ferrous or ferric form with the possible exception of the effect of long term alcohol on the segmental absorption of ferric Fe59.

The Effect of Administered Oestrogens and Androgens on the Absorption of Iron by Rats

Summary. The effects of injected oestrogens and androgens on the absorption of iron by mature female rats have been studied. Whereas there was no significant change with oestrogen despite falling haemoglobin levels, there was a slight but significant increase with androgen. These changes probably result from changes in iron stores secondary to changes in erythropoiesis.

The Effect of Injected Iron on the Absorption of Iron in Iron Deficiency

Summary. The effects of injected iron on the absorption of iron by iron deficient animals have been studied. When iron deficiency is partly or fully corrected by injection of iron dextran, prolonged and marked depression of iron absorption follows despite the persistence of iron deficiency. Although total absorption increases with decreasing size of parenteral replacement dose, the depression of absorption per unit of iron injected increases.

The Effects on Iron Absorption of Gastro‐intestinal Secretions from Patients with Iron‐Deficiency Anaemia and Haemochromatosis

We have investigated the effects of gastric and intestinal secretions from normal human subjects and those with iron‐deficiency anaemia and haemochromatosis on the in vitro uptake of 59Fe by everted rat gut segments. Whereas gastric secretions from normal subjects did not potentiate the uptake, intestinal secretions did. This effect was more marked with gastric and intestinal secretions obtained from patients with iron‐deficiency anaemia and haemochromatosis, but it disappeared with prior heating. These findings suggest that gastric and intestinal secretions play a role in the regulation of iron absorption.

Use of isolated subcutaneous intestinal loops for direct study of intestinal absorption of radioisotopes in dogs

SummaryDirect counting of the disappearance rate of radioactivity of a gamma-emitting isotope placed in a blind intestinal loop located in the subcutaneous tissue provides a good measure of the absorption of that isotope. The problems encountered in the preparation of these loops and their use in absorption studies are discussed. It is noted in passing that significant absorption of iron may take place from the colon.