Nguyen Van Cong

Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs

A lambda gt11 cDNA library constructed from human tracheo-bronchial mucosa was screened with a polyclonal antiserum raised to chemically deglycosylated pronase glycopeptides from human bronchial mucins. Out of 20 positives clones, one partial cDNA clone was isolated and allowed to map a novel human tracheo-bronchial mucin gene. It contains 48 nucleotide tandem repeats quite perfectly identical which encodes a protein containing about 50% of hydroxy amino-acids. This clone hybridized to polydisperse messages produced by human tracheo-bronchial and human colonic mucosae. The gene (proposed name MUC 4) from which cDNA is derived maps to chromosome 3.

Mapping of the gene for anti-Müllerian hormone to the short arm of human chromosome 19

The gene coding for human anti-Müllerian hormone (AMH) was localized to subbands p13.2----p13.3 on chromosome 19, using in situ hybridization and Southern blot analysis of a panel of man-mouse and man-hamster somatic cell hybrids.

Assignment of human tracheobronchial mucin gene(s) to 11p15 and a tracheobronchial mucin-related sequence to chromosome 13

SummaryExtensive heterogeneity of tracheobronchial mucin RNAs has been described recently. Based on the results of total or partial cDNA sequencing, the mucin cDNAs obtained were classified into three groups. The first group contained 24 bp tandem repeat sequences, the second exhibited homology at their amino- and carboxyl-terminals, and the third group seems to consist of alternative hydrophilic-hydrophobic zones. JER58, JER47 and JER57 probes, representing the first, second, and third tracheobronchial mucin families respectively, were used for chromosome assignment. In human DNAs digested with BamHI, the JER58 probe detected a sequence of 21 kb, the JER47 probe detected a major sequence of 21 kb and a minor sequence of 4 kb, and the JER57 probe detected two sequences of 1.8kb and 1.3kb. By somatic hybrid cell analysis, the JER58, JER47, and JER57 major sequences were assigned to chromosome 11 and the JER47 minor sequence to chromosome 13. By in situ hybridization the JER58, JER47 and JER57 probes were assigned to 11p15. Under the experimental conditions used, no specific hybridization to the chromosome 13 region was observed with the JER47 probe. Our results indicate that tracheobronchial mucin gene(s) is/are localized on 11p15. The minor JER47 BamHL sequnce localized on chromosome 13 probably corresponds to a tracheal-mucin related sequence. The intestinal mucin gene was also recently localized to the same 11p15 region. Intestinal and tracheobronchial mucins appear different according to their tissue distribution and their cDNA nucleotide sequences. Tracheal mucin probes (JER58, JER47, JER57) and intestinal probes may represent independent genes on 11p15 or else different mRNAs from the same primary transcript produced by differential splicing. Further studies using mucin genomic probes for 11p15 will be required for the elucidation of tracheal and intestinal mucin gene organisation in this region.

The human placental protein 14 (PP14) gene is localized on chromosome 9q34

SummaryPP14 protein (placental protein 14) is abundantly secreted by the human endometrium under the influence of progesterone. Human PP14 is homologous to β-lactoglobulin, the main component of equine, bovine, and ovine milk whey. A genomic PP14 probe (PP14G1) was used for the chromosome assignment of the PP14 gene. Somatic hybrid cells enabled PP14G1 to be assigned to chromosome 9. In situ hybridization further refined this assignment to 9q34. The localization of the PP14 gene in the region of the ABO locus is consistent with the linkage described in bovines between beta-lactoglobulin and the J blood group (homologous to the human ABO group).

Localization of the human oncogene SPI1 on chromosome 11, region p11.22

SummarySpi1 is an oncogene specifically activated in acute murine erythroleukemias induced by the Friend spleen focus forming virus (SFFV). Three probes were used for the chromosomal assignment of the human SPI1 oncogene: cDb1 and RaB2 correspond respectively to murine Spi1 and human SPI1 cDNA probes; C45a6B probe is a murine genomic DNA sequence located in the Spi1 5′ region and is known as a major SFFV integration site in murine erythroleukemia cells. Somatic hybrid cells enabled cDb1 and RaB2 to be assigned to chromosome 11. The murine C45a6B probe, which is not included in the Spi1 gene, detected a homologous sequence on human chromosome 11. RaB2 was assigned to 11p 11.22 by in situ hybridization. Three human genes known between 11p11 and 11p13 (FSHB, CAT, ACP2) were on murine chromosome 2. Therefore, the localization of human SPI1 on 11p11.22 was consistent with the assignment of the Spi1 oncogene to murine chromosome 2.

ASSIGNMENT OF THE HUMAN CD9 GENE TO CHROMOSOME 12 (REGION P13) BY USE OF HUMAN SPECIFIC DNA PROBES

SummaryWe have used human specific DNA probes and somatic hybrid cell analysis to localize the human CD 9 gene to chromosome 12 (region p13).

Chromosome No. 1 of man and Chimpanzee: Identity of gene mapping for three loci: PPH, PGM1, and Pep-C

SummaryCytogenetic and biochemical analysis of 10 independant Chimpanzee-Mouse cell hybrids and of 18 subclones of one of these showed that PPH, PGM1 and Pep-C are localized on the Chimpanzee chromosome homologous to the human chromoosome No. 1.

Comparison of the chromosomal maps of chimpanzee and man

Results are reported of a study of Y-chromatin of lymphocytes and granulocytes in the peripheral blood of 100 pregnant women. In women who later delivered a male child, a mean of 3.75 % Y-chromatin was found in lymphocytes and 2.8 % in granulocytes. The paternal Y-chromatin was studied before investigating the maternal blood smears, thus defining the Y-chromatin. Nonetheless, even with this method the rate of false diagnosis (14 %) in prenatal sex-diagnosis could be reduced only moderately. Most mistakes were caused by aggregations of strong fluorescent autosomal segments simulating a Y-chromatin in size and frequency. In early pregnancy lymphoid cells with a Y-chromatin could first be found in the 8th week; granulocytes showing a Y-chromatin appeared in the 9th week. The methods used here are not reliable enough to be used in genetic counseling.