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Featured researches published by Ni Zheng.


Chinese Science Bulletin | 2006

Rescued virus from infectious cDNA clone of rabbit hemorrhagic disease virus is adapted to RK13 cells line

Liu GuangQing; Ni Zheng; Yun Tao; Zhang Yu-ying; Du Qingyun; Sheng Zutian; Liang Huali; Hua Jionggang; Li ShuangMao; Chen JianPing

Based on the infectious full-length cDNA clone of rabbit hemorrhagic disease virus (RHDV), the in vitro transcripts are introduced into RK13 cells. 12 h later, CPE could be observed clearly, and virual antigen could also be detected by IFA. The titre of the recovered virus is 104.6/mL. Immune electron microscopic observation of the virus particles revealed that the particles were rotund with a diameter of about 30 nm. Besides, virus titre quantification obtained by qRT-PCR showed a correlation between time from infection and virus titre. All these results showed that we have recovered RHDV from RK13 cells by reverse genetics technology successfully, and this would be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and new type vaccines of RHDV.


Archive | 2014

Recombinant porcine pseudorabies virus, as well as construction method and application thereof

Zhang Cun; Cui Shangjin; Ye Weicheng; Chen Liu; Yu Bin; Ni Zheng; Yun Tao; Hua Jionggang; Zhang Yan


Chinese journal of virology | 2009

Studies on the apoptosis of RK13 cells induced by rabbit hemorrhagic disease virus.

Ni Zheng; Wei Wei; Liu GuangQing; Chen Liu; Yu Bin; Yun Tao; Hua Jionggang; Li ShuangMao


Archive | 2015

Duck tembusu virus (DTMUV) protein E linear B cell antigenic epitope polypeptide and application thereof

Yu Bin; Zhang Cun; Ni Zheng; Hua Jionggang; Chen Liu; Yun Tao; Ye Weicheng


Archive | 2017

Truncated gene of duck Tembusu virus E protein and recombinant duck plaque virus, and construction method and application thereof

Chen Liu; Zhang Cun; Yu Bin; Hua Jionggang; Ni Zheng; Ye Weicheng; Yun Tao


Nongye Shengwu Jishu Xuebao | 2016

RT-PCR法の確立と応用におけるアヒル I遺伝子型I II 型Jian別の診断と応用【JST・京大機械翻訳】

Yun Tao; Zhang Cun; Hua Jionggang; Yu Bin; Ye Weicheng; Chen Liu; Ni Zheng


Archive | 2016

Primers and method for identifying genotype I and genotype II DRVs (duck reoviruses)

Yun Tao; Zhang Cun; Hua Jionggang; Ye Weicheng; Yu Bin; Chen Liu; Ni Zheng


Archive | 2015

Recombinant duck plague virus for expressing gosling plague virus VP2 and its constructing method and application

Chen Liu; Zhang Cun; Ni Zheng; Ye Weicheng; Yu Bin; Yun Tao; Hua Jionggang


Archive | 2015

Recombinant duck plague virus of expressing duck tembusu virus E protein as well as construction method and application of recombinant duck plague virus

Chen Liu; Zhang Cun; Ni Zheng; Ye Weicheng; Yu Bin; Yun Tao; Hua Jionggang


Archive | 2015

Antigen protein for detecting duck hemorrhagic ovaritis virus antibody and application thereof

Yu Bin; Zhang Cun; Yun Tao; Chen Liu; Ni Zheng; Hua Jionggang; Ye Weicheng

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Chen Liu

Zhejiang Normal University

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Zhang Cun

Nanjing Agricultural University

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Song Yi

Zhejiang Normal University

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Zhang Yanming

University of Agriculture

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