Nicha Charoensri
Khon Kaen University
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Publication
Featured researches published by Nicha Charoensri.
Journal of Antimicrobial Chemotherapy | 2012
Benchamas Rimrang; Aroonwadee Chanawong; Aroonlug Lulitanond; Chotechana Wilailuckana; Nicha Charoensri; Pipat Sribenjalux; Waewta Phumsrikaew; Lumyai Wonglakorn; Anusak Kerdsin; Ploenchan Chetchotisakd
OBJECTIVES To detect carbapenemases in clinical isolates of Enterobacteriaceae collected from patients in a university hospital in Thailand between October 2010 and August 2011. METHODS A total of 4818 Enterobacteriaceae isolates were screened for the presence of carbapenemases by ertapenem and imipenem disc diffusion tests. All positive screening isolates were subjected to modified Hodge test, phenylboronic acid- and EDTA-carbapenem combined disc tests and two multiplex PCRs of bla(IMP), bla(VIM), bla(SPM), bla(SIM) and bla(GIM), and of bla(KPC), bla(NDM) and bla(OXA-48). Carbapenemase-producing isolates were typed by PFGE and then characterized by antimicrobial susceptibility tests. Conjugation was performed using a broth culture mating method. RESULTS Two isolates each of Escherichia coli, Klebsiella pneumoniae and Citrobacter freundii produced NDM-1, whereas two other isolates of K. pneumoniae produced IMP-14a. DNA fingerprints revealed that the metallo-β-lactamase (MBL)-producing isolates were of different strains except for clonal strains of C. freundii. In vitro transfer of carbapenem resistance was successful for the eight MBL-producing isolates. All MBL producers were susceptible to colistin and tigecycline. The six NDM-producing isolates were recovered from the urine of three patients, who had no history of travel outside Thailand. Interestingly, one patient had chronic urinary tract infections caused by a K. pneumoniae strain and two strains of E. coli producing NDM-1. CONCLUSIONS Surveillance of carbapenemases, particularly NDM-1, in Enterobacteriaceae is urgently needed to control and prevent the spread of these resistance determinants in our country.
International Journal of Infectious Diseases | 2009
Peechanika Chopjitt; Tipaya Ekalaksananan; Chamsai Pientong; Bunkerd Kongyingyoes; Pilaiwan Kleebkaow; Nicha Charoensri
OBJECTIVES To investigate the prevalence of HPV, HPV16, and HPV16 variants in scraped cervical cells cytologically diagnosed as normal cervical cell and in formalin-fixed, paraffin-embedded tissues of cervical intraepithelial neoplasia II-III and squamous cervical carcinoma in Northeast Thailand. METHODS All samples were subjected to PCR using consensus GP5+/GP6+ primers. HPV16 was genotyped by Southern blot hybridization and reverse line blot hybridization. The HPV16 E6 gene was amplified and sequenced. RESULTS HPV infections were found in 33.8% of normal cervical cells, 97.3% of cervical intraepithelial neoplasia II-III, and 100% of squamous cervical carcinomas. The prevalence of HPV16 increased significantly with histological grade (normal cervical cell, 16.7%; cervical intraepithelial neoplasia II-III, 38.9%; squamous cervical carcinoma, 75%). The most common variant found was the Asian (As) (58.7%) followed by the European (E) lineage (41.3%). The HPV16 As lineages showed a risk association in 73.9% of squamous cervical cancer and 57.1% of cervical intraepithelial neoplasia II-III, while no increased risk was observed in the E lineages. CONCLUSION Our study demonstrates that HPV16, in particular the As variant, was the major causative agent associated with cervical cancer in Northeast Thailand, and our study suggests that some mutations of the E6 gene in this variant, which leads to amino acid changes, may be more carcinogenic.
Vaccine | 2015
Amporn Suphatrakul; Thippawan Yasanga; Poonsook Keelapang; Rungtawan Sriburi; Thaneeya Roytrakul; Rojjanaporn Pulmanausahakul; Utaiwan Utaipat; Yanee Kawilapan; Chunya Puttikhunt; Watchara Kasinrerk; Sutee Yoksan; Prasert Auewarakul; Prida Malasit; Nicha Charoensri; Nopporn Sittisombut
Recent phase IIb/III trials of a tetravalent live attenuated vaccine candidate revealed a need for improvement in the stimulation of protective immunity against diseases caused by dengue type 2 virus (DENV-2). Our attempts to develop particulate antigens for possibly supplementing live attenuated virus preparation involve generation and purification of recombinant DENV-2 virus-like particles (VLPs) derived from stably (prM+E)-expressing mosquito cells. Two VLP preparations generated with either negligible or enhanced prM cleavage exhibited different proportions of spherical particles and tubular particles of variable lengths. In BALB/c mice, VLPs were moderately immunogenic, requiring adjuvants for the induction of strong virus neutralizing antibody responses. VLPs with enhanced prM cleavage induced higher levels of neutralizing antibody than those without, but the stimulatory activity of both VLPs was similar in the presence of adjuvants. Comparison of EDIII-binding antibodies in mice following two adjuvanted doses of these VLPs revealed subtle differences in the stimulation of anti-EDIII binding antibodies. In cynomolgus macaques, VLPs with enhanced prM cleavage augmented strongly neutralizing antibody and EDIII-binding antibody responses in live attenuated virus-primed recipients, suggesting that these DENV-2 VLPs may be useful as the boosting antigen in prime-boost immunization. As the levels of neutralizing antibody induced in macaques with the prime-boost immunization were comparable to those infected with wild type virus, this virus-prime VLP-boost regimen may provide an immunization platform in which a need for robust neutralizing antibody response in the protection against DENV-2-associated illnesses could be tested.
Journal of Virological Methods | 2014
Nicha Charoensri; Amporn Suphatrakul; Rungtawan Sriburi; Thippawan Yasanga; Jiraphan Junjhon; Poonsook Keelapang; Utaiwan Utaipat; Chunya Puttikhunt; Watchara Kasinrerk; Prida Malasit; Nopporn Sittisombut
Recombinant virus-like particles (rVLPs) of flaviviruses are non-infectious particles released from cells expressing the envelope glycoproteins prM and E. Dengue virus rVLPs are recognized as a potential vaccine candidate, but large scale production of these particles is hindered by low yields and the occurrence of cytopathic effects. In an approach to improve the yield of rVLPs from transfected insect cells, several components of a dengue serotype 2 virus prM+E expression cassette were modified and the effect of these modifications was assessed during transient expression. Enhancement of extracellular rVLP levels by simultaneous substitutions of the prM signal peptide and the stem-anchor region of E with homologous cellular and viral counterparts, respectively, was further augmented by codon optimization. Extensive formation of multinucleated cells following transfection with the codon-optimized expression cassette was abrogated by introducing an E fusion loop mutation. This mutation also helped restore the extracellular E levels affected negatively by alteration of a charged residue at the pr-M junction, which was intended to promote maturation of rVLPs during export. Optimized expression cassettes generated in this multiple add-on modification approach should be useful in the generation of stably expressing clones and production of dengue virus rVLPs for immunogenicity studies.
Journal of Antimicrobial Chemotherapy | 2017
Arpasiri Srisrattakarn; Aroonlug Lulitanond; Chotechana Wilailuckana; Nicha Charoensri; Jureerut Daduang; Aroonwadee Chanawong
Objectives: To develop a simple gold nanoparticle (AuNP)‐based colorimetric test, GoldNano Carb (GoldC), for detecting carbapenemase production in Gram‐negative bacteria, compared with updated Carba NP (CNP) and CarbAcineto NP (CAcNP) tests by using PCR methods as gold standard. Methods: Ninety‐nine carbapenemase‐producing Enterobacteriaceae (CPE), Pseudomonas spp. and Acinetobacter spp. isolates and 89 non‐CPE isolates were tested by the GoldC and CNP. Additionally, the CAcNP was performed in the Acinetobacter spp. isolates. The final imipenem (imipenem/cilastatin form) concentration was 5 mg/mL for all three tests. For the GoldC, the imipenem powder was added directly to bacterial cell suspension in distilled water prior to detection of acid product by the citrate‐capped AuNP solution. An AuNP change from red to purple, blue or green indicates carbapenemase activity. Results: The GoldC detected all carbapenemase producers except one OXA‐23‐like producer (99.0% sensitivity), whereas 11 carbapenemase producers (10 Acinetobacter and 1 P. aeruginosa) were CNP negative (88.9% sensitivity). However, the GoldC and CNP provided 100% and 98.6% sensitivity, respectively, for the CPE and Pseudomonas spp. Both tests gave one false positive from CTX‐M‐1‐like‐producing Enterobacter spp. (98.9% specificity). The GoldC and CAcNP detected 96.7% and 93.3% of the Acinetobacter spp. isolates, respectively. Interestingly, times to positivity by the GoldC were markedly shorter than those by the CNP (76.8% versus 36.2% positive at 5 min) and CAcNP (43.3% at 5 min versus 20% within 30 min). Conclusions: The GoldC is fast, easy, highly sensitive and inexpensive (˜
Japanese Journal of Infectious Diseases | 2017
Sawitree Nuramrum; Aroonwadee Chanawong; Kamonwan Lunha; Aroonlug Lulitanond; Arunnee Sangka; Chotechana Wilailuckana; Sunpetch Angkititrakul; Nicha Charoensri; Lumyai Wonglakorn; Prajuab Chaimanee; Ploenchan Chetchotisakd
0.25 per test), suggesting that it may be suitable for routine carbapenemase detection in low‐resource settings for infection control or epidemiological purposes.
Diagnostic Microbiology and Infectious Disease | 2016
Kamonwan Lunha; Aroonwadee Chanawong; Aroonlug Lulitanond; Chotechana Wilailuckana; Nicha Charoensri; Lumyai Wonglakorn; Pimjai Saenjamla; Prajuab Chaimanee; Sunpetch Angkititrakul; Ploenchan Chetchotisakd
Twelve nonreplicate carbapenemase-negative ertapenem (ETP)-nonsusceptible (CNENS) Escherichia coli isolates obtained at a Thai university hospital between 2010 and 2014 were characterized and compared with 2 carbapenemase-producing E. coli isolates from the same hospital. Eight unique pulsed-field gel electrophoresis patterns were obtained. All the isolates produced CTX-M-15 β-lactamase and 2 either coexpressed CMY-2 cephalosporinase or showed increased efflux pump activity. Amino acid sequence analysis revealed that an OmpF defect (in 7 isolates) due to mutations generating truncated proteins or an IS1 insertion was more prevalent than a defect in OmpC was (no truncated proteins detected). Seven out of 10 isolates possessing OmpC variants with any OmpF defect were weakly ETP-resistant (minimum inhibitory concentrations [MICs] of 1-4 μg/mL) and imipenem (IPM)- and meropenem (MEM)-susceptible (MICs 0.125-0.5 μg/mL). Two isolates with ompC PCR-negative results and an OmpF defect showed higher carbapenem MICs (8-32, 1-8, and 1-4 μg/mL for ETP, IPM, and MEM, respectively) with the highest MICs associated with the additional efflux pump activity. Both carbapenemase producers possessing CTX-M-15 and a porin background identical to that in the CNENS isolates showed ETP, IPM, and MEM MICs of 128-256, 8, and 2-32 μg/mL, respectively. These findings suggest that a porin defect combined with CTX-M-15 production is the major mechanism of low carbapenem susceptibility among our CNENS isolates, which have potential to become strongly carbapenem-resistant because of additional carbapenemase or efflux pump activities.
World Journal of Microbiology & Biotechnology | 2016
Arpasiri Srisrattakarn; Aroonlug Lulitanond; Chotechana Wilailuckana; Nicha Charoensri; Lumyai Wonglakorn; Sirikan Piyapatthanakul; Ampai Supajeen; Aroonwadee Chanawong
Archive | 2017
Santi Sakdarat; Sujittapron Sittiso; Tipaya Ekalaksananan; Chamsai Pientong; Nicha Charoensri; Bunkerd Kongyingyoes
World Journal of Microbiology & Biotechnology | 2017
Arpasiri Srisrattakarn; Aroonlug Lulitanond; Chotechana Wilailuckana; Nicha Charoensri; Lumyai Wonglakorn; Pimjai Saenjamla; Prajuab Chaimanee; Jureerut Daduang; Aroonwadee Chanawong