Nicholaos I. Limberopoulos

Advantages of microsphere-assisted super-resolution imaging technique over solid immersion lens and confocal microscopies

We demonstrate a series of advantages of microsphere-assisted imaging over confocal and solid immersion lens microscopies including intrinsic flexibility, better resolution, higher magnification, and longer working distances. We discerned minimal feature sizes of ∼50-60 nm in nanoplasmonic arrays at the illumination wavelength λ = 405 nm. It is demonstrated that liquid-immersed, high-index (n ∼ 1.9-2.1) spheres provide a superior image quality compared to that obtained by spheres with the same index contrast in an air environment. We estimate that using transparent microspheres at deep UV wavelengths of ∼200 nm might make possible imaging of various nanostructures with extraordinary high ∼30 nm resolution.

Super‐resolution microscopy by movable thin‐films with embedded microspheres: Resolution analysis

Microsphere-assisted imaging emerged as a surprisingly simple way of achieving optical super-resolution imaging. In this work, we use movable PDMS thin films with embedded high-index barium titanate glass microspheres a sample scanning capability was developed, thus removing the main limitation of this technology based on its small field-of-view.

Movable thin films with embedded high-index microspheres for super-resolution microscopy

Microsphere-assisted imaging emerged as a surprisingly simple way of achieving optical super-resolution imaging. In this work, we use movable PDMS thin films with embedded high-index barium titanate glass microspheres a sample scanning capability was developed, thus removing the main limitation of this technology based on its small field-of-view.

Overcoming the diffraction limit of imaging nanoplasmonic arrays by microspheres and microfibers.

Super-resolution microscopy by microspheres emerged as a simple and broadband imaging technique; however, the mechanisms of imaging are debated in the literature. Furthermore, the resolution values were estimated based on semi-quantitative criteria. The primary goals of this work are threefold: i) to quantify the spatial resolution provided by this method, ii) to compare the resolution of nanoplasmonic structures formed by different metals, and iii) to understand the imaging provided by microfibers. To this end, arrays of Au and Al nanoplasmonic dimers with very similar geometry were imaged using confocal laser scanning microscopy at λ = 405 nm through high-index (n~1.9-2.2) liquid-immersed BaTiO3 microspheres and through etched silica microfibers. We developed a treatment of super-resolved images in label-free microscopy based on using point-spread functions with subdiffraction-limited widths. It is applicable to objects with arbitrary shapes and can be viewed as an integral form of the super-resolution quantification widely accepted in fluorescent microscopy. In the case of imaging through microspheres, the resolution ~λ/6-λ/7 is demonstrated for Au and Al nanoplasmonic arrays. In the case of imaging through microfibers, the resolution ~λ/6 with magnification M~2.1 is demonstrated in the direction perpendicular to the fiber with hundreds of times larger field-of-view in comparison to microspheres.

Microsphere-chain waveguides: Focusing and transport properties

It is shown that the focusing properties of polystyrene microsphere-chain waveguides (MCWs) formed by sufficiently large spheres (D ≥ 20λ, where D is the sphere diameter and λ is the wavelength of light) scale with the sphere diameter as predicted by geometrical optics. However, this scaling behavior does not hold for mesoscale MCWs with D ≤ 10λ resulting in a periodical focusing with gradually reducing beam waists and in extremely small propagation losses. The observed effects are related to properties of nanojet-induced and periodically focused modes in such structures. The results can be used for developing focusing microprobes, laser scalpels, and polarization filters.

Spectrally resolved resonant propulsion of dielectric microspheres

Use of resonant light forces opens up a unique approach to high-volume sorting of microspherical resonators with much higher uniformity of resonances compared to that in coupled-cavity structures obtained by the best semiconductor technologies. In this work, the spectral response of the propulsion forces exerted on polystyrene microspheres near tapered microfibers is directly observed. The measurements are based on the control of the detuning between the tunable laser and internal resonances in each sphere with accuracy higher than the width of the resonances. The measured spectral shape of the propulsion forces correlates well with the whispering-gallery mode resonances in the microspheres. The existence of a stable radial trap for the microspheres propelled along the taper is demonstrated. The giant force peaks observed for 20-μm spheres are found to be in a good agreement with a model calculation demonstrating an efficient use of the light momentum for propelling the microspheres.

Formation of polarized beams in chains of dielectric spheres and cylinders

Using numerical modeling, it is shown that chains of dielectric spheres and cylinders act as polarizers. The mechanism is based on gradual filtering of periodically focused modes with a certain polarization propagating with minimal losses due to Brewster angles conditions, whereas orthogonally polarized modes are strongly attenuated. It is shown that chains of cylinders filter linearly polarized beams, whereas chains of spheres filter radially polarized beams. In the geometrical optics limit, we show that in a range of sphere refractive indices 1.68-1.80 a degree of radial polarization in excess of 0.9 can be obtained in 10-sphere-long chains.

Increasing sensitivity and angle-of-view of mid-wave infrared detectors by integration with dielectric microspheres

We observed up to 100 times enhancement of sensitivity of mid-wave infrared photodetectors in the 2–5 μm range by using photonic jets produced by sapphire, polystyrene, and soda-lime glass microspheres with diameters in the 90–300 μm range. By finite-difference time-domain (FDTD) method for modeling, we gain insight into the role of the microspheres refractive index, size, and alignment with respect to the detector mesa. A combination of enhanced sensitivity with angle-of-view (AOV) up to 20° is demonstrated for individual photodetectors. It is proposed that integration with microspheres can be scaled up for large focal plane arrays, which should provide maximal light collection efficiencies with wide AOVs, a combination of properties highly attractive for imaging applications.

Super-resolution imaging by arrays of high-index spheres embedded in transparent matrices

We fabricated thin-films made from polydimethylsiloxane (PDMS) with embedded high-index (n~1.9-2.2) microspheres for super-resolution imaging applications. To control the position of microspheres, such films can be translated along the surface of the nanoplasmonic structure to be imaged. Microsphere-assisted imaging, through these matrices, provided lateral resolution of ~λ/7 in nanoplasmonic dimer arrays with an illuminating wavelength λ=405 nm. Such thin films can be used as contact optical components to boost the resolution capability of conventional microscopes.

Optical microscopy with super-resolution by liquid-immersed high-index microspheres

We study super-resolution capability of liquid-immersed high refractive index (n~1.9–2.1) barium titanate glass microspheres with diameters from several microns up to hundreds of microns. Imaging is provided in a conventional upright microscope with the spheres placed in a contact position with various semiconductor and metallic nanostructures. Using a commercial Blu-ray disk, we demonstrate an ability to discern 100 nm feature sizes which cannot be resolved by conventional microscopy. Using silver nanowires with diameter about 100 nm, we demonstrate ~1.7 times improvement in spatial resolution compared to conventional diffraction-limited far field microscopy. Using two-dimensional nanoplasmonic arrays, we demonstrate high resolution imaging by using objectives with surprisingly small numerical apertures. The last property is attractive for high-resolution imaging at long working distances. This imaging technique can be used in biomedical microscopy, microfluidics, and nanophotonics applications.