Nicholas Rupcich
McMaster University
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Nicholas Rupcich.
Analytica Chimica Acta | 2003
Nicholas Rupcich; John D. Brennan
Abstract We report on the development of a new class of protein microarrays based on the co-immobilization of multiple components within a single pin-printed sol–gel array element. In the first case, the microarraying of a coupled two enzyme reaction involving glucose oxidase and horseradish peroxidase along with the fluorogenic reagent Amplex Red is demonstrated to allow “reagentless” fluorimetric detection of glucose. The second system involved the detection of urea using co-immobilized urease and fluorescein dextran, which works on the basis of a pH induced change in fluorescein emission intensity upon production of ammonium carbonate owing to hydrolysis of urea. In both the cases, it is demonstrated that the changes in intensity from the array are time-dependent, consistent with the enzyme catalyzed reaction, showing that such arrays can be used for kinetic studies. The rate of intensity change was also found to be dependent on the concentration of analyte added to the array, showing that such arrays could be useful for quantitative multianalyte biosensing. Inhibition of urease by the competitive inhibitor thiourea is also demonstrated on a microarray, demonstrating that sol–gel-based microarrays may find use in high-throughput drug-screening applications.
Archive | 2009
Nicholas Rupcich; Razvan Nutiu; Yutu Shen; Yingfu Li; John D. Brennan
The past 15 years have seen a revolution in the area of functional nucleic acid (FNA) research since the demonstration that single-stranded RNA and DNA species can be used for both ligand binding and catalysis. An emerging area of application for such species is in the development of solid-phase fluorimetric assays for biosensing, proteomics, and drug screening purposes. In this chapter, the methods for immobilization of functional nucleic acids are briefly reviewed, with emphasis on emerging technologies such as sol-gel encapsulation. Methods for generating fluorescence signals from aptamers and nucleic acid enzymes are then described, and the use of such species in solid-phase fluorimetric assays is then discussed. Unique features of sol-gel based materials for the development of solid-phase assays are highlighted, and some emerging applications of immobilized FNA species are discussed.
Analytical Chemistry | 2005
Nicholas Rupcich; Razvan Nutiu; Yingfu Li; John D. Brennan
Journal of the American Chemical Society | 2006
Nicholas Rupcich; William Chiuman; Razvan Nutiu; Shirley Mei; Kulwinder K. Flora; Yingfu Li; John D. Brennan
Analytical Chemistry | 2007
Yutu Shen; Gillian Mackey; Nicholas Rupcich; Darin Gloster; William Chiuman; Yingfu Li; John D. Brennan
Angewandte Chemie | 2006
Nicholas Rupcich; Razvan Nutiu; Yingfu Li; John D. Brennan
Chemistry of Materials | 2003
Nicholas Rupcich; and Aaron Goldstein; John D. Brennan
Archive | 2003
John D. Brennan; Nicholas Rupcich
Analytical Chemistry | 2005
Nicholas Rupcich; James R. A. Green; John D. Brennan
Archive | 2005
Nicholas Rupcich; Razvan Nutiu; John D. Brennan; Yingfu Li