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Featured researches published by Niraj Shende.


Indian Journal of Clinical Biochemistry | 2003

Serodiagnosis of tuberculosis using two ELISA systems.

Swati Banerjee; Sonika Gupta; Niraj Shende; Satish Kumar; B. C. Harinath

Serodiagnosis by ELISA has been widely explored over the years, in the diagnosis of tuberculosis. Two ELISA systems were evaluated for detection of mycobacterial antibodies in pulmonary and extra pulmonary tuberculosis. The two test assays explored were ERBA LISA (TB IgG) test (Anda Biologicals) which uses A60 antigen complex found in the cytosol of typical and atypical mycobacteria, and SEVA TB (IgG) ELISA, which uses a 31 kDa, glycoprotein antigen purified fromM. tb H37Ra culture filtrate. Sera from 98 proven tuberculosis [pulmonary TB (48), tuberculous lymphadenopathy (30), tuberculous meningitis (15) & genitourinary TB (5)] were studied along with 32 healthy controls. The overall positivity obtained using ERBA LISA (TB IgG) test and SEVA TB (IgG) ELISA test was 72.9% and 91.6% in pulmonary tuberculosis, 43.3% and 76.6% in tuberculous lymphadenopathy respectively. The sensitivity of ERBA LISA test in tuberculous meningitis and genito-urinary TB was significantly low (26.6% & 40% respectively) compared to sensitivity obtained using SEVA TB ELISA (86.6% & 60% respectively) with overall specificity of 60% and 87.5%. Thus SEVA TB IgG ELISA test was found to be more sensitive than ERBA LISA in detecting IgG antibodies in tuberculous sera, in particular in extra pulmonary tuberculosis cases.


Indian Journal of Medical Microbiology | 2008

A low molecular weight ES-20 protein released in vivo and in vitro with diagnostic potential in lymph node tuberculosis

Niraj Shende; Vijay Upadhye; Satish Kumar; B. C. Harinath

PURPOSE To determine role of antigens released in vivo and in vitro in immunodiagnosis of tuberculosis (TB). METHODS In vivo released circulating tuberculosis antigen (CTA) was obtained from TB sera by ammonium sulphate precipitation and in vitro released excretory-secretory (ES) antigens from Mycobacterium tuberculosis culture filtrate. CTA and ES antigens were fractionated by SDS-PAGE and electro-eluted gel fractions were analysed for antigen by ELISA. RESULTS Low molecular weight proteins CTA-9 and ES-9 showed high titre of antigen activity. To explore the diagnostic potential of low molecular weight ES antigen, M. tuberculosis ES antigen was further fractionated by gel filtration chromatography followed by purification on anion exchange column using fast protein liquid chromatography and a highly seroreactive ESG-5D (ES-20) antigen was obtained. Competitive inhibition showed that CTA-9 and ES-9 antigens inhibit the binding of ES-20 antigen to its antibody. Seroanalysis showed sensitivity of 83 and 80% for ES-20 antigen and antibody detection, respectively, in pulmonary TB and 90% in lymph node TB. CONCLUSIONS Seroreactivity studies using M. tuberculosis ES-20 antigen showed usefulness in detection of TB; in particular, lymph node TB.


Indian Journal of Clinical Biochemistry | 2002

Analysis of SEVA TB ES-31 antigen specific immunoglobulins IgM, IgA and IgG in sera of sputum and culture positive pulmonary tuberculosis.

Sonika Gupta; Niraj Shende; Swati Banerjee; Satish Kumar; M. V. R. Reddy; B. C. Harinath

Tuberculosis remains major health problem in India and developing countries Immunodiagnosis has important role in screening, diagnosis and management of tuberculosis. SEVA TB ES-31 antigen has shown potential in detecting tuberculous IgG antibody in earlier studies from our laboratory. In the present study we have analysedSEVA TB ES-31 antigen specific immunoglobulinsIgM, IgA and IgG in clinically and bacteriologically confirmed pulmonary tuberculosis cases to determine the usefulness of specific immunoglobulin class in the diagnosis of patients attending the hospital.Of the 30 cases of pulmonary tuberculosis 25 (83.3%) were positive for IgG, 19 (63.3%) for IgM and 16 (53.3%) for IgA. On combining IgG and IgM positivity, sensitivity was increased to 93.3%. While combining IgG and IgA positivity, sensitivity increased to 90%. However specificity was decreased to 66.6% and 70% for both of these combinations respectively. It could be envisaged from this study that IgG antibody detection against ES-31 antigen showed acceptable sensitivity (83.3%) and specificity (86.6%) compared to IgM or IgA alone or in combination. When immune responses were analysed according to degree of sputum positivity, IgG response was observed to be predominant in all grades, compared to IgM or IgA antibody. The addition of IgM or IgA as an adjunct test increases the sensitivity but at the cost of specificity. Hence the detection of IgG alone is more useful compared to IgM or IgA assay, in detecting tuberculosis disease cases coming to the hospital.


Diagnostic Microbiology and Infectious Disease | 2006

A cocktail of affinity-purified antibodies reactive with diagnostically useful mycobacterial antigens ES-31, ES-43, and EST-6 for detecting the presence of Mycobacterium tuberculosis

B. C. Harinath; Satish Kumar; Santa Saha Roy; Suvarna Hirudkar; Vijay Upadhye; Niraj Shende


Medical Science Monitor | 2005

IgG subclass antibody response to mycobacterial serine protease at differentstages of pulmonary tuberculosis.

Sonika Gupta; Niraj Shende; Amerjeet Singh Bhatia; Satish Kumar; Baskar C. Harinath


Current Science | 2005

Detection of antibodies to a cocktail of mycobacterial excretory-secretory antigens in tuberculosis by ELISA and immunoblotting

Sonika Gupta; Niraj Shende; Satish Kumar; B. C. Harinath


Biomedical Research-tokyo | 2007

Detection of antibody and antigen in extrapulmonary tuberculosis patients' sera using a cocktail of mycobacterial excretory secretory antigens and their antibodies

Vijay Upadhye; Niraj Shende; Satish Kumar; B. C. Harinath


Indian Journal of Experimental Biology | 2005

Effectivity of crude versus purified mycobacterial secretory proteins as immunogen for optimum antibody production.

Santa Saha-Roy; Niraj Shende; Satish Kumar; B. C. Harinath


Indian Journal of Experimental Biology | 2007

Isolation of Mycobacterium tuberculosis protein antigens ES-31, ES-43 and EST-6 of diagnostic interest from Tubercle Bacilli by affinity chromatography

Vijay Upadhye; Santa Saha-Roy; Niraj Shende; Satish Kumar; B. C. Harinath


Indian Journal of Experimental Biology | 2008

Detection of in vitro and in vivo released antigens of diagnostic interest in Mycobacterium tuberculosis by immunoblotting.

Niraj Shende; Sonika Gupta; Vijay Upadhye; Satish Kumar; B. C. Harinath

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Satish Kumar

Council of Scientific and Industrial Research

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B. C. Harinath

Mahatma Gandhi Institute of Medical Sciences

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Sonika Gupta

Mahatma Gandhi Institute of Medical Sciences

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Vijay Upadhye

Mahatma Gandhi Institute of Medical Sciences

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Swati Banerjee

Mahatma Gandhi Institute of Medical Sciences

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Amerjeet Singh Bhatia

Mahatma Gandhi Institute of Medical Sciences

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Baskar C. Harinath

Mahatma Gandhi Institute of Medical Sciences

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M. V. R. Reddy

Mahatma Gandhi Institute of Medical Sciences

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Santa Saha Roy

Mahatma Gandhi Institute of Medical Sciences

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Suvarna Hirudkar

Mahatma Gandhi Institute of Medical Sciences

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