Nishimori Katsuhiko

Expression of cloned calf prochymosin gene sequence in Escherichia coli

Abstract An expression plasmid for calf prochymosin (prorennin) cDNA was constructed. The plasmid (pCR301) contains the lac UV5 promoter in front of the fused gene in which the codons for the N-terminal four amino acids of prochymosin cDNA were replaced with those for the N-terminal ten amino acids of β-galactosidase. Synthesis of the fused protein with the expected M r was detected immunologically in Escherichia coli harboring pCR301. The product seemed to be localized in the cell membrane of the bacterial host.