Niu Na

Differential Proteomics on Multi-Ovary and Mono-Ovary Trait of Wheat

Muliti-ovary is a favorable trait that might be a solution for increasing the yield of hybrid seed in wheat.In this study, the differential proteins of panicles at tetrad stage were detected between the mono-ovary line 77(2)and its near-isogenic multi-ovary line Mu77(2)using SDS-PAGE method.Approximately 450 protein spots were observed in the 2-DE gels within the area of pH 4–7 and molecular weight 14.4–97.4 kD,of which 30 were identified as differential protein spots with at least double expression levels and 99%statisticalty significant difference.Six specific protein spots with differential expressions were then analyzed using LC-MS/MS method.The result showed that they are glycine-rich RNA-binding protein(S1),SGT1-1(S2), HMG-I/Y protein(S3),glutathione transferase(S4),fructose-bisphosphate aldolase(S5),and unnamed protein(S6).These proteins are involved in the regulation of DNA transcription,protein translation,energy metabolism,signal transduction,plants defense,and other resistance function,and might be related to the formation of multi-ovary in wheat.

Editing Sites in Transcript of Mitochondrial atp6 Gene of Male Sterile Line with Aegilops kotschyi Cytoplasm in Wheat

RNA editing of atp6 gene at different stages of pollen development was sequenced directly and from clones using the cytoplasmic male-sterile line ms(Kots)-90-110(A) and its near-isogenic line(NIL,BC5F2).The DNA sequences of ms(Kots)-90-110(A) and the NIL had 99% identities with those of common wheat(Triticum aestivum) and T.timopheevi.There were 15 editing sites in the conservative regions of atp6 transcripts between male sterile and fertile lines,of which 13 occurred at the first or the second position of codons with alteration of amino acid type,and two occurred at the third position with no change of amino acid type.Codens at the sixth and seventh sites were cotranscripted.The frequency of the editing site was increased gradually as the develop-mental stage of anther proceeded.The restorer gene in BC5F2 obviously increased the editing frequency at each editing site.Tran-scripts that were inadequately edited might affect the normal mitochondria function.RNA editing of atp6 is probably associated with CMS in wheat with Aegilops kotschyi cytoplasm.