Nobuaki Mashimo

A radioimmunoassay of thromboxane B2 with thromboxane B2-125I-tyramide and its application to the study on the thromboxane B2 formation during platelet aggregation

Abstract A radioimmunoassay for measuring thromboxane B2 with thromboxane B2-125I-tyramide was developed. Antibody to thromboxane B2 that was produced in rabbits immunized with conjugates of thromboxane B2 coupled to bovine serum albumin had a high specificity to thromboxane B2 Thromboxane B2-125I-tyramide had a high affinity to antiplasma of thromboxane B2. This method was utilized to study thromboxane B2 formation during platelet aggregation induced by collagen, ADP and adrenalin. Formations of thromboxane B2 were observed in accordance with platelet secondary aggregation, namely, release reaction.

Microassay of adenine nucleotides in intima and media of the aortic wall

Abstract Combining a quantitative histochemistry method with the Firefly luciferase method we found that adenosine-triphosphate (ATP) could be accurately measured in 100 to 200 μg of aortic tissue. Trichloracetic acid solution was used for extraction of ATP and by so doing, weighing at the freezing point or neutralization by (potassium hydroxide) KOH in case of perchloric acid could be eliminated. Simultaneous assay of cyclic nucleotides was thus feasible. Contents of ATP and ADP in intima and media of aortic wall of rabbits, cows, and pigs and levels of these nucleotides in atherosclerotic lesions of aorta of rabbits fed a 1% cholesterol containig diet for 15 weeks were measured. Levels of adenine nucleotides in atherosclerotic lesions of rabbits were low compared to levels in intima of the aortic wall. Acquisition of these data is vital for monitoring changes in levels of adenine nucleotides in relation to pathological changes in tissues.

A case of testicular tumor with an abdominal mass, multiple pulmonary metastatic lesions, and apparently normal testes.

18才,男牲.腹部不定愁訴があり来院.肺野の多発性結節性陰影,腹部腫瘤を指摘され入院した.腹部CTで,膵頭部領域に腫瘤を認めた.睾丸腫大,女性化乳房,尿妊娠反応陽性等の睾丸腫瘍を思わせる所見はなく,特異的な腫瘍マーカーの有意な上昇はみられなかった.十分な検査,治療ができぬまま,喀血を契機に全身状態が悪化し,患者は入院第27病日で死亡.剖検では,右睾丸に小指頭大の腫瘍を認め,肺,肝,副腎に広範な転移を認めた.腹部腫瘤は,リンパ節転移巣の集塊であった.睾丸の原発巣は, choriocarcinomaとembryonal carcinomaから成り,後者が優位で,転移巣はchoriocarcinomaのみで占められていた. PAP法では, choriocarcinomaはHCG陽性であった.これら二成分から成る睾丸腫瘍はまれで,身体所見,腫瘍マーカー,過剰ホルモンの検索では,診断が確定できなかった.超音波検査等による検索がこのような睾丸腫瘍の診断には併用されるべきであろう.