Nobuhiro Morone
Nagoya University
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Publication
Featured researches published by Nobuhiro Morone.
Journal of Cell Biology | 2006
Nobuhiro Morone; Takahiro K. Fujiwara; Kotono Murase; Rinshi S. Kasai; Hiroshi Ike; Shigeki Yuasa; Jiro Usukura; Akihiro Kusumi
Three-dimensional images of the undercoat structure on the cytoplasmic surface of the upper cell membrane of normal rat kidney fibroblast (NRK) cells and fetal rat skin keratinocytes were reconstructed by electron tomography, with 0.85-nm–thick consecutive sections made ∼100 nm from the cytoplasmic surface using rapidly frozen, deeply etched, platinum-replicated plasma membranes. The membrane skeleton (MSK) primarily consists of actin filaments and associated proteins. The MSK covers the entire cytoplasmic surface and is closely linked to clathrin-coated pits and caveolae. The actin filaments that are closely apposed to the cytoplasmic surface of the plasma membrane (within 10.2 nm) are likely to form the boundaries of the membrane compartments responsible for the temporary confinement of membrane molecules, thus partitioning the plasma membrane with regard to their lateral diffusion. The distribution of the MSK mesh size as determined by electron tomography and that of the compartment size as determined from high speed single-particle tracking of phospholipid diffusion agree well in both cell types, supporting the MSK fence and MSK-anchored protein picket models.
Genes to Cells | 2004
Saeko Kawabata; Jiro Usukura; Nobuhiro Morone; Masaaki Ito; Akihiro Iwamatsu; Kozo Kaibuchi; Mutsuki Amano
Rho‐kinase and myosin phosphatase cooperatively regulate the phosphorylation level of myosin light chain and are involved in the formation of stress fibres and smooth muscle contraction. Rho‐kinase has been known to be localized at stress fibres, but little is known about the mechanism of its localization. Here we identified non‐muscle myosin heavy chain IIA and IIB as the pleckstrin homology domain‐interacting molecules by affinity column chromatography. The pleckstrin homology domain of Rho‐kinase binds to myosin II directly in in vitro cosedimentation assay. The C‐terminal region of the pleckstrin homology domain was important for this interaction, and the point mutations in the pleckstrin homology domain mutant (W1170A, W1340L) resulted in a decrease in the binding. We also found that the pleckstrin homology domain, but not the pleckstrin homology domain mutant (W1170A, W1340L), was localized at stress fibres in fibroblasts. These results indicate that Rho‐kinase is localized at stress fibres through binding of the pleckstrin homology domain to myosin II.
Archive | 2006
Keiko Tamiya-Koizumi; Takashi Murate; Katsumi Tanaka; Yuji Nishizawa; Nobuhiro Morone; Jiro Usukura; Yoshio Hirabayashi
Much attention has been paid to the roles of sphingomyelin (SM) metabolism in the regulation of various cell functions such as cell growth, differentiation and apoptosis. Sphingomyelinase (SMase) catalyzes the first step of SM-metabolizing pathways that generate the bioactive metabolite, ceramide. SMase present in membrane microdomains, raft and caveolae may be involved in the agonist-mediated events. However, it is still unclear which molecular species of SMase is stimulated by an agonist to produce ceramide in membrane microdomains, and how agonist-sensitive SMase and SM are topologically localized within the micro-domains. Here, we first show the close interaction between neutral SMase and caveolin 1 in 1% Triton X-100-insoluble fractions of plasma membranes isolated from adult rat resting liver and rapidly growing rat ascites hepatoma, AH 7974 cells. Then, we describe the connection between acid SMase and caveolin 2 in cell differentiation or apoptosis induced by all-trans retinoic acid. Finally, we discuss the possible roles for caveolins with respect to the topological distribution of SMase and SM in caveolae.
Developmental Cell | 2005
Atsushi Enomoto; Hideki Murakami; Naoya Asai; Nobuhiro Morone; Takashi Watanabe; Kumi Kawai; Yoshiki Murakumo; Jiro Usukura; Kozo Kaibuchi; Masahide Takahashi
Journal of Nanoscience and Nanotechnology | 2007
Shinji Moritake; Shu Taira; Yuko Ichiyanagi; Nobuhiro Morone; Si Young Song; Takahiro Hatanaka; Shigeki Yuasa; Mitsutoshi Setou
Archive | 2006
Akihiro Kusumi; Kenichi Suzuki; Junko Kondo; Nobuhiro Morone; Yasuhiro Umemura
生物物理 | 2009
Takuya Kobayashi; Yoshitaka Kimori; Nobuhiro Morone; Takashi Murayama
生物物理 | 2008
Fumi Wakui; Tomoko Kohno; Reiko Fukumura-Kato; Yoshitaka Kimori; Kotaro Hattori; Shigeki Yuasa; Nobuhiro Morone
生物物理 | 2008
Nobuhiro Morone; Takahiro Fujiwara; Rinshi S. Kasai; Shigeki Yuasa; Jiro Usukura; A. Kusumi
Journal of the Neurological Sciences | 2007
Ikuko Yao; Hiroshi Takagi; Hiroshi Ageta; Tomoaki Kahyo; Showbu Sato; Ken Hatanaka; Yoichi Fukuda; Tomoki Chiba; Nobuhiro Morone; Shigeki Yuasa; Kaoru Inokuchi; Toshihisa Ohtsuka; Grant R. MacGregor; Keiji Tanaka; Mitsutoshi Setou