Nobumasa Kobayashi

Clinical significance of LEA-1 expression in adult acute myeloid leukemia

In this study, we examined expressions of several adhesion molecules (AdMs), i.e. leukocyte function antigen-1 (LFA-1: CD11a/CD18), Hermes homing receptor (CD44) and intercellular adhesion molecule-1 (ICAM-1: CD54), on leukemia cells from 51 adult patients with newly diagnosed acute myeloid leukemias (AMLs) to elucidate clinical significance of these AdM expressions. Those expressions in lymphoid malignancies have been correlated with tumor evolutions, but CD44 was detected in all the AML cases examined and CD54 expression did not associate with their clinical characteristics or outcomes. However, we found that LFA-1 expressions significantly correlated with splenomegaly, resistance to induction chemotherapies and short survival periods in AML patients.

Serum Apolipoprotein H Levels in Systemic Lupus Erythematosus Are Not Influenced by Antiphospholipid Antibodies

Anticardiolipin antibodies (aCL) were recently discovered to recognize a complex consisting of phospholipids and apolipoprotein H (apo H). In this study, we determined the serum apo H levels in 36 systemic lupus erythematosus (SLE) patients with or without antiphospholipid antibodies (aPL), including aCL and lupus anticoagulants, to clarify the possible effects of aPL on apo H levels in vivo. The apo H levels were low in SLE patients as compared with 22 healthy controls. However, no associations were found between apo H levels and circulating aPL or clinical features of the antiphospholipid antibody syndrome. A secondary hyperlipidemic state, which probably related to lupus nephritis (proteinuria) and/or prednisolone treatment, increased apo H levels in SLE patients.

Macrophage–T Cell Interaction Is Essential for the Induction of p75 Interleukin 2 (IL–2) Receptor and IL–2 Responsiveness in Human CD4+ T Cells

Fresh human CD8+ T cells showed a strong proliferative response to a high concentration of interleukin 2 (IL–2) in the absence of macrophages. In contrast, CD4+ T cells revealed no significant IL–2 responsiveness in the absence of macrophages. However, if CD4+ T cells were cocultured with macrophages, they showed higher proliferative response to IL–2 than CD8+ T cells. In accordance with the magnitude of IL–2 responsiveness, freshly isolated CD8+ T cells expressed significant amounts of p75 IL–2 receptor, while fresh CD4+ T cells did not express p75 IL–2 receptor. The expression of p75 IL–2 receptor on CD4+ T cells was induced by coculture with macrophages. The macrophage–induced p75 IL–2 receptor acquisition was blocked by monoclonal antibody (inAh) against class II antigen. Moreover, the addition of anti–CD4 mAb or anti–class II mAb to the culture caused a great inhibition of IL–2 responsiveness of CD4+ T cells. These results strongly suggest that macrophage–T cell interaction through CD4 and/or class II molecules is essential for the expression of p75 IL–2 receptor and IL–2 responsiveness in human CD4+, but not CD8+ T cells.

Flow Cytometric Analysis of T-Cell-Rich B-Cell Lymphoma

Flow Cytometric Analysis of T-Cell-Rich B-Cell Lymphoma H. Hiroshi Kawada S. Shigeki Watanabe M. Miyoko Yoshida R. Ryuki Fukuda N. Nobumasa Kobayashi A. Akira Masumoto Y. Yoshiaki Ogawa Y. Yoshiaki Ohbayashi S. Shuji Yonekura Y. Yukinobu Ichikawa Fourth Department of Internal Medicine, Tokai University School of Medicine, and Blood Transfusion Center of Tokai University Hospital, Bohseidai, Isehara, Kanagawa, Japan

Upregulation of cell surface expression of T-lymphoid antigens and adhesion molecules on acute myeloid leukaemia cells after in vivo administration of granulocyte colony-stimulating factor.

We herein report a case of acute myeloid leukaemia (AML, FAB:M0) who showed upregulation of T-lymphoid antigens (CD2, CD7) and adhesion molecules (CD11a, CD11b, CD18) on leukaemic cells after in vivo administration of granulocyte colony-stimulating factor (G-CSF). To our knowledge, this is the first report which describes in vivo changes of cell surface antigen expression on AML cells after the administration of G-CSF.