Nobuo Homma

Flow cytometry analysis of α 1-adrenoceptor subtypes

To characterize the α 1‐adrenoceptor subtypes, we developed a flow cytometry method using the fluorescent ligand BODIPY‐FL prazosin and the anti‐peptide antibody against the α 1b‐adrenoceptor amino terminus (designated 1B‐N1‐C) as probes. Three α 1‐adrenoceptors (α 1a, β 1b and α 1d) expresed in CHO cells were detected by BODIPY‐FL prazosin; however, only α 1b‐adrenoceptor subtype was detected by the anti‐peptide antibody 1B‐N1‐C. Furthermore, the flow cytometry analysis with 1B‐N1‐C specifically identified α 1b‐adrenoceptor in native cells of hamster DDT1‐MF2 cells, rat hepatocytes and1 cardiomyocytes.