Norihiko Hata

Dendrimer-Immobilized Culture Surface as a Tool to Evaluate Formation of Cellular Cytoskeleton of Anchorage-Dependent Cells

Anchorage-dependent cultivation of human epithelial and keratinocyte cells was carried out on surfaces modified with synthesized dendrimers. Notable elongation of the epithelial cells was recognized on the culture surface immobilized with a dendrimer having D-glucose as a functional ligand, but not when a dendrimer having L-glucose was used or when the dendrimer was ligand-free. This morphological change was attributable to a temporary grasping of the cells at the D-glucose moiety via a glucose transporter-mediated mechanism present in the cell membrane. Following visualization of the actin filaments of the cells, it was considered that the cellular elongation on the D-glucose-bound dendrimer surface reflected the degree of formation of the cellular cytoskeleton. The cellular roundness was calculated by means of image analysis of the individual cells and employed as a parameter to evaluate the formation of the cellular cytoskeleton. In the culture of keratinocytes on the D-glucose-bound dendrimer surface, it was demonstrated that the decrease in the ratio of elongated cells (i.e., cells with a low roundness value) was correlated with the deterioration in the growth potential associated with cellular senescence.

Relations between individual cellular motions and proliferative potentials in successive cultures of human keratinocytes.

AbstractIn the successive cultures of human keratinocyte cells, cellular motions of extension and rotation were analyzed based on observation of the individual cells, to evaluate the proliferative potential in a whole cell population. In lag phases of the serial cultures, an extension index of individual cells, RE, was defined as an average spreading rate divided by initial cell area for each cell. The mean value of RE was found to relate to prolongation of lag time; namely it decreased with increasing passage number in the successive cultures approaching cellular senescence. During the courses of the cultures, the rotation rate of paired cells was also measured through time-lapse observation. The mean value of rotation rate,

Cell culture apparatus, cell culture method, cell culture program and cell culture system

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Cell Culture Shaking Device and Shaking Culture Method as Cell Culture Method

, decreased with an increase in doubling time caused by the progress of cellular age, reaching an almost constant value of

Comprehension of attachment and multiplication properties by observing individual cell behaviors in anchorage-dependent culture

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Cell culture estimating system, method of cell culture estimation and cell culture estimating program

h-1 in the cultures with prolonged doubling time of over 59 h. It was concluded that the indices determined from the motions of individual cells, RE and