Norio Kudo

An immunohistochemical study on the embryonic development of renin-containing cells in the mouse and pig.

The prenatal occurrence and distribution of renin‐containing (RC) cells were investigated immunohistochemically in mouse and pig embryos.

Distribution of Endocrine Cells in Duck Digestive Tracts

食道から直腸末端までのアヒル消化管における内分泌細胞の存在と分布を,鉛ヘマトキシリン,塩酸トルイジンブルー,Sevier and Mungerの鍍銀法およびMassonの銀親和反応を用いて検索した。 鉛ヘマトキシリンと塩酸トルイジンブルーで染まる細胞は食道を除く消化管の全部位でみられ,好銀性細胞は腺胃粘膜,腺胃腺および腸管の全部位に,銀親和性細胞は腸管にのみみられた。 内分泌細胞の分布密度は,筋胃幽門部が最も多く,次が腺胃腺であり,腸は十二指腸,空腸,回腸,盲腸,結直腸の5部位とも同程度で幽門部の約1/2,腺胃粘膜および胃峡部は腺胃腺の1/10,筋胃中央部は前2部位よりも少なく最少であった。 筋胃幽門部は,筋胃と十二指腸を分けるわずかな粘膜ひだから筋胃側5㎜位の部分であり,この部に非銀親和性で非好銀性の内分泌細胞が高密度に存在することは興味ある所見である。 これら内分泌細胞の染色性および分布から,アヒル消化管における4種類以上の内分泌細胞の存在が推測された。

Immunohistochemical Studies on the Antigen Localization and the Germinal Center Formation in Mouse Lymph Nodes

The antigen, 2, 4-dinitrophenylated and alum-precipitated bovine serum albumin (DNP-AP-BSA), was inoculated from the mouse foot pads into popliteal lymph nodes. The DNP-BSA-bearing phagocytes were first found in the paracortex, which was the thymus (T)-dependent area, at 3 hr after inoculation. Subsequently, at 1 week after inoculation, germinal centers containing a few T cells were formed within the lymphoid follicles in close contact with the T-dependent area. At 2 weeks after inoculation, crescent-shaped networks of DNP-BSA accompanying the IgG were observed in the peripheral region of germinal centers. These findings show that the cooperation of phagocytes, T cells and B cells against thymus dependent antigens may be indispensable in the formation of germinal centers and in the retention of their function in lymphocyte proliferation.