Norma Suely Evangelista-Barreto

Aeromonas spp. isolated from oysters (Crassostrea rhizophorea) from a natural oyster bed, Ceará, Brazil

Between April and October 2002, thirty fortnightly collections of oysters (Crassostrea rhizophorea) from a natural oyster bed at the Cocó River estuary in the Sabiaguaba region (Fortaleza, Ceará, Brazil) were carried out, aiming to isolate Aeromonas spp. strains. Oyster samples were submitted to the direct plating (DP) and the presence/absence (P/A) methods. Aeromonas were identified in 15 (50%) samples analyzed by the DP method and in 13 (43%) analyzed by the P/A method. A. caviae, A. eucrenophila, A. media, A. sobria, A. trota, A. veronii bv. sobria, A. veronii bv. veronii and Aeromonas sp. were isolated. The predominant species was A. veronii (both biovars), which was identified in 13 (43%) samples, followed by A. media in 11 (37%) and A. caviae in seven (23%). From the 59 strains identified, 28 (48%) presented resistance to at least one of the eight antibiotics tested.

Cometabolic Decolorization of the Reactive Azo Dye Orange II by Geobacillus stearothermophilus UCP 986

The decolorization of solutions containing the textile reactive dye Orange II by the bacteria Geobacillus stearothermophilus (UCP 986) was investigated. The experiments were statistically evaluated by two-level-four factor (2 4) full factorial design, as aeration, azo dye concentration, inoculum size, and culture media. The best results were with Luria Bertani (LB) medium under aeration, and the decolorization efficiency meant that 96—98% of the azo dye was removed after 24 h of incubation. The efficiency of LB medium in color reduction was due to a co-metabolic process. Sulphanilic acid (4-aminobenzene sulphonic acid) was not detected. The testing for toxicity using the brine shrimp Artemia salina showed the absence of toxic metabolites during the decolorization process; however, when the concentration of the dye and agitation were high (0.050 mM and 150 rpm) the shrimp showed high mortality rates (LC50 (lethal dose — assay concentration able to cause 50% of death) 49.28% v/v). The parameters such as culture medium and agitation are significantly important to the decolorization of the Orange II process. G. stearothermophilus (UCP 986) showed a high biotechnology potential for textile bioremediation of azo dye under the co-metabolic process.

Probiotic properties of lactic acid bacteria isolated from human milk

The objective of this study was to identify and characterize lactic acid bacteria isolated from human milk, with an emphasis on their probiotic properties.

Population dynamics and antimicrobial susceptibility of Aeromonas spp. along a salinity gradient in an urban estuary in Northeastern Brazil

The main objective of this study was to quantify population and identify culturable species of Aeromonas in sediment and surface water collected along a salinity gradient in an urban estuary in Northeastern Brazil. Thirty sediment samples and 30 water samples were collected from 3 sampling locations (A, B and C) between October 2007 and April 2008. The Aeromonas count was 10-7050CFU/mL (A), 25-38,500CFU/mL (B) and<10CFU/mL (C) for water samples, and ∼100-37,500CFU/g (A), 1200-43,500CFU/g (B) and<10CFU/g (C) for sediment samples. Five species (Aeromonas caviae, A. sobria, A. trota, A. salmonicida and A. allosaccharophila) were identified among 41 isolates. All strains were sensitive to chloramphenicol and ceftriaxone, whereas 33 (80, 4%) strains were resistant to at least 2 of the 9 antibiotics tested. Resistance to erythromycin was mostly plasmidial. In conclusion, due to pollution, the Cocó River is contaminated by pathogenic strains of Aeromonas spp. with a high incidence of antibacterial resistance, posing a serious risk to human health.

Genes of virulence and antimicrobial resistance in Vibrio parahaemolyticus prevalent in areas of ostreiculture

This study aimed to quantify Vibrio spp. and evaluate the profile of antimicrobial susceptibility and virulence factors in Vibrio parahaemolyticus strains in the samples of oysters collected from two estuaries of the Baixo Sul, Bahia. The samples were collected between June 2015 and January 2016 from natural banks (E1) (oyster) and from cultivation areas (E2) (water and oyster). For the quantification of Vibrio spp., the most probable number (MPN) was determined and the characterization of V. parahaemolyticus marker (species-specific) gene tl was performed. Pathogenicity was observed with the Kanagawa test, and the presence of the tdh, trh and ure genes were tested. The antimicrobial sensitivity test included disc diffusion method with 15 antimicrobial discs, β-lactamase enzyme production, and the presence of blaTEM, blaSHV, and blaCTX-M. The maximum density of Vibrio spp. in the samples from cultivation was 4.70 log MPN g-1 and extractivism was 6.10 log MPN g-1, with the temperature being the most influencing variable on the presence of microorganisms in the cultivation area (E2). The tl gene was detected in 71% of the isolates, without the presence of tdh, trh and ure genes. All strains of V. parahaemolyticus were Kanagawa negative. High antimicrobial resistance was observed in the β-lactam antibiotics (cephalothin - 72%, ampicillin - 60%) and aminoglycosides (amikacin - 64%), with multi-resistance in 88% of the strains of V. parahaemolyticus, of which 68% of the resistance was mediated by plasmids. Phenotypically, no production of β-lactamase enzymes was observed, but the presence of blaTEM genes was observed. The multiresistant character of plasmids reported in V. parahaemolyticus strains increases the concern about native bacteria in the marine environment since it can potentially compromise the control of this bacterium infection in bivalve mollusks.This study aimed to quantify Vibrio spp. and evaluate the profile of antimicrobial susceptibility and virulence factors in Vibrio parahaemolyticus strains in the samples of oysters collected from two estuaries of the Baixo Sul, Bahia. The samples were collected between June 2015 and January 2016 from natural banks (E1) (oyster) and from cultivation areas (E2) (water and oyster). For the quantification of Vibrio spp., the most probable number (MPN) was determined and the characterization of V. parahaemolyticus marker (species-specific) gene tl was performed. Pathogenicity was observed with the Kanagawa test, and the presence of the tdh, trh and ure genes were tested. The antimicrobial sensitivity test included disc diffusion method with 15 antimicrobial discs, β-lactamase enzyme production, and the presence of blaTEM, blaSHV, and blaCTX-M. The maximum density of Vibrio spp. in the samples from cultivation was 4.70 log MPN g-1 and extractivism was 6.10 log MPN g-1, with the temperature being the most influencing variable on the presence of microorganisms in the cultivation area (E2). The tl gene was detected in 71% of the isolates, without the presence of tdh, trh and ure genes. All strains of V. parahaemolyticus were Kanagawa negative. High antimicrobial resistance was observed in the β-lactam antibiotics (cephalothin 72%, ampicillin 60%) and aminoglycosides (amikacin 64%), with multi-resistance in 88% of the strains of V. parahaemolyticus, of which 68% of the resistance was mediated by plasmids. Phenotypically, no production of β-lactamase enzymes was observed, but the presence of blaTEM genes was observed. The multiresistant character of plasmids reported in V. parahaemolyticus strains increases the concern about native bacteria in the marine environment since it can potentially compromise the control of this bacterium infection in bivalve mollusks.

Artisan cheese as vehicle of contamination Escherichia coli and coagulase-positive staphylococci antimicrobial resistance

Dissertação do Programa de Pós-graduação em Microbiologia Agrícola, Universidade Federal do Recôncavo da Bahia UFRB Doutora em Ciências Biológicas, Universidade Federal do Recôncavo da Bahia UFRB, nsevangelista@yahoo.com.br Agrônoma, Discente do Programa de Pós-graduação em Microbiologia Agrícola, Universidade Federal do Recôncavo da Bahia UFRB, gleydecordova@hotmail.com Discente de Biologia, Universidade Federal do Recôncavo da Bahia UFRB, jeferson.pesca@gmail.com Agrônoma, Bolsista PNPD/CAPES, Universidade Federal do Recôncavo da Bahia UFRB, fsbernardes@yahoo.com.br Biológa, Programa de Pós-graduação em Ciências Agrárias, Universidade Federal do Recôncavo da Bahia UFRB, ____________________________________________________________________________ Resumo: Este trabalho avaliou a presença de bactérias mesófilas cultiváveis (BMC), fungos filamentosos e leveduras, coliformes a 35 oC e a 45 oC, Escherichia coli, Salmonella e estafilococos coagulase positiva, bem como o perfil de resistência antimicrobiana entre os isolados bacterianos em queijos artesanais (n = 28) produzidos e comercializados em Cruz das Almas, Bahia. O número de microrganismos patogênicos diferiu estatisticamente (p > 0,05) entre os queijos avaliados. O queijo de coalho apresentou as maiores contagens de microrganismos, com 80% de contaminação por estafilococos coagulase positiva. Escherichia coli foi encontrada em 50% das amostras de queijo de coalho e em 7% no queijo de manteiga. Não foi observada a presença de Salmonella. Escherichia coli apresentou resistência a ampicilina (16,7%) e imipenem (16,7%). Estafilococos coagulase positiva foi resistente a nitrofurantoína (55,6%), tetraciclina (88,9%) e vancomicina (77,7%), apresentando multirresistência. A qualidade microbiológica dos queijos artesanais comercializados em Cruz das Almas é insatisfatória para o consumo, além de veicular patógenos multiresistentes. Palavras-chave: queijo de coalho, queijo de manteiga, antimicrobianos. Abstract: This study evaluated the presence of cultivable mesophilic bacteria (CMB), filamentous fungi, yeasts and coliforms at 35 oC and at 45 oC, Escherichia coli, Salmonella and Revista Brasileira de Higiene e Sanidade Animal Brazilian Journal of Hygiene and Animal Sanity ISSN: 1981-2965

Antimicrobial Activity of Some Lactic Acid Bacteria Isolated From Human Milk

Nayara Alves Reis, Norma Suely Evangelista-Barreto, Margarete Alice Fontes Saraiva, Elaine Araujo de Carvalho, Adriana Pereira Sampaio, Alessandra Santana Silva. Antimicrobial Activity of Some Lactic Acid Bacteria Isolated From Human Milk. In: Anais do 12o Congresso Latinoamericano de Microbiologia e Higiene de Alimentos MICROAL 2014 [= Blucher Food Science Proceedings, num.1, vol.1]. Sao Paulo: Editora Blucher, 2014. DOI 10.5151/foodsci-microal-021 Antimicrobial Activity of Some Lactic Acid Bacteria Isolated From Human Milk

Antimicrobial Resistance of Lactic Acid Bacteria Isolated From Human Milk

Nayara Alves Reis, Norma Suely Evangelista-Barreto, Margarete Alice Fontes Saraiva (II), Marly Silveira Santos, Adriana Pereira Sampaio, Alessandra Santana Silva. Antimicrobial Resistance of Lactic Acid Bacteria Isolated From Human Milk. In: Anais do 12o Congresso Latinoamericano de Microbiologia e Higiene de Alimentos MICROAL 2014 [= Blucher Food Science Proceedings, num.1, vol.1]. Sao Paulo: Editora Blucher, 2014. DOI 10.5151/foodsci-microal-305 Antimicrobial Resistance of Lactic Acid Bacteria Isolated From Human Milk