Norman Ende

Murine survival of lethal irradiation with the use of human umbilical cord blood

We have found that human umbilical cord blood (HUCB) will routinely protect mice exposed to lethal levels of irradiation. At the end of 50 days, over seventy percent (70%) of mice injected with HUCB survived 900 cGy or irradiation, which produced 100% deaths in the uninjected control animals. Moreover, there was some evidence that human colony stimulating factors further improved survival. Anti-Natural Killer cell (NK) antibody was utilized along with HUCB in these studies, however, Anti-NK cell serum alone had no radioprotective effect in mice. The studies reported here suggest the possibility of utilizing HUCB for immediate protection of humans from lethal irradiation.

Purification and characterization of a mouse mast cell esteroprotease

Abstract An attempt has been made to isolate, purify, and study the properties of the esteroprotease obtained from neoplastic mouse mast cells. This study revcals that the esteroprotease can be disassociated by DEAE-cellulose chromatography into esterase and protease subunits. However, there is some evidence that suggests that this might represent a macromolecule. Various studies are presented on the esterase and protease subunits as to purification, kinetics, stability, substrate specificity, influence of H ion, Ca ion ion, and inhibitors. The enzyme was found to differ significantly from mouse trypsin and also differs from the trypsin-like enzyme reported in dog and human mast cell. In addition, the esteroprotease enzyme was found to lyse human fibrin and hydrolyze protein in human pleural effusion fluids.