Norman L. Goodman

Pathogenicity of the basidiospores of Filobasidiella neoformans

The pathogenicity of the purified basidiospores of Filosbasidiella neoformans was determined. The basidiospores were purified by successive filtration and inoculated into white Swiss female mice via the intracranial and intravenous route. At autopsy, infection was seen with as few as fifty inoculated cells. The brain, liver and spleen were examined culturally and histologically to prove the pathogenicity of the purified basidiospores.

Peritonitis Due to Exophiala jeanselmei in a Patient Undergoing Continuous Ambulatory Peritoneal Dialysis

We describe the second reported case of peritonitis caused by the fungus Exophiala jeanselmei in a patient on continuous ambulatory peritoneal dialysis (CAPD) and the first such patient to survive. Management of the patient consisted of Tenckhoff catheter removal and intravenous amphotericin B.

Pathogenicity of the hyphae of Filobasidiella neoformans.

The pathogenicity of the purified hyphae of Filobasidiella neoformans was determined. The hyphal particles were inoculated into white Swiss female mice via the intracranial and intravenous route. Upon autopsy, infection was seen with as few as 25 hyphal elements. The brain, liver, and spleen were examined culturally and the brain examined histologically to prove the pathogenicity of the purified hyphae.

Cryptococcal infection of the temporal bone

Cryptococcus neoformans is a yeast-like organism which, after inhalation, may cause pulmonary disease or a systemic fungemia associated with numerous extrapulmonary sites of infection, but most notably affecting the central nervous system. Despite this frequent involvement of the leptomeninges, only three cases of infection of the temporal bones have been previously described. We describe an additional case with comments on the possible significance of infection of this site.

Technique for the purification of the hyphae of Filobasidiella neoformans

A technique for the purification of the hyphae ofFilobasidiella neoformans is described. Cultural conditions and strains for maximal hyphal production were determined. Blastospores were separated from hyphae by sonification of mycelial suspensions. Following this, the initial density ranges of all cell types were determined by isopycnic centrifugation, using Renografin-60 as a supporting medium. Based on these data, rate centrifugation with different density ranges was used to obtain cell separation. The final percentage of non-hyphal cells to total population was 0.007%. These were determined to be still viable at the end of separation.

Obituary for Richard (Dick) Harry Kruse

Richard (Dick) Harry Kruse, 73, long-time microbiologist and mycologist died May 31, at his home in Georgetown, Kentucky. Dick, a native of Gilman, VT, had a distinguished career at the Fort Detrick Army Biological Laboratories from 1953–1972. He became well known for his expertise in biosafety and with Dr Arnold Wedum published seminal data on the hazards of working with microbiological agents in the laboratory. When, in 1972, the Fort Detrick Army Biological Laboratories closed, Dick was Chief of the Research Section, Industrial Health and Safety Division. In 1973 Dick moved to Kentucky to become Director of the Mycology Laboratory, Kentucky State Respiratory Disease Hospital in Paris. Here he became