Norman Whitaker Green

Stereophotography as a tool in aquatic biology

Abstract The details of an underwater stereophotographic system is outlined. This system is based on 35 mm cameras, using high-resolution colour film. A modular construction enables adaptation of the photographic system to a wide range of applications. Interfaced to a computerised data base, the system offers quick and fairly accurate information feedback on the investigated biological communities. Objects down to about 0.5mm natural size are detectable. Spatial relationships and the state of the organisms are easily documented. By time-lapse photography, changes in aquatic communities, e.g. due to pollution impacts, may be well monitored.

Blue mussels (Mytilus edulis spp.) as sentinel organisms in coastal pollution monitoring: A review

The blue mussel (Mytilus spp.) is widely used as a bioindicator for monitoring of coastal water pollution (mussel watch programs). Herein we provide a review of this study field with emphasis on: the suitability of Mytilus spp. as environmental sentinels; uptake and bioaccumulation patterns of key pollutant classes; the use of Mytilus spp. in mussel watch programs; recent trends in Norwegian mussel monitoring; environmental quality standards and background concentrations of key contaminants; pollutant effect biomarkers; confounding factors; particulate contaminants (microplastics, engineered nanomaterials); climate change; harmonization of monitoring procedures; and the use of deployed mussels (transplant caging) in pollution monitoring. Lastly, the overall state of the art of blue mussel pollution monitoring is discussed and some important issues for future research and development are highlighted.

Relationships Between Physiology, Tissue Contaminants, and Biomarker Responses in Atlantic Cod (Gadus morhua L.)

It is generally assumed that there should be some relationship between tissue residues of contaminants and related biomarker responses in the same individual, with a possible additional modulation through physiological processes. The main basis for such assumptions originated from experimental studies, but it has been less easy to detect and quantify any such relationships in field‐collected material. The aim of the current study was to clarify whether the responses of selected biomarkers (cytochrome P-4501A [CYP1A], δ-aminolevulinic acid dehydratase [ALA-D], metallothionein [MT]) could be correlated with physiological parameters and/or contaminant levels. Atlantic cod (Gadus morhua L.) were sampled over a decade at a number of locations along the Norwegian coast. The locations included both polluted and reference areas. In addition to contaminant-related responses (biomarkers), physiological characteristics were registered for each individual and the contaminant load determined. There were clear relationships between responses in all three biomarkers and tissue contaminants, but additional factors such as age or size and liver lipid or liver somatic index also contributed in multiple-regression models. The results indicated that responses in all three biomarkers reflected relevant, contaminant-related processes in the cod even though none of them provided a simple separation between polluted and pristine locations.

Regression models for assessing trends in cadmium and PCBs in cod livers from the Oslofjord

Abstract Trend studies of contaminants measured in fish liver have expressed concentrations on different bases (e.g. liver-weights, dry-weights or fat-weights) and regressed them against different variables. This makes the results of studies difficult to compare. A simple model is presented which shows that various combinations of bases and variables are equivalent. The model is used to assess trends in cadmium and PCBs in cod livers from the Oslofjord for 1981ndash;1986. Implications of the fitted equations for future data collection are considered.

Post World War II orcharding creates present day DDT-problems in The Sorfjord (Western Norway): A case study

The Sørfjord has a long history of agriculture and industry, and environmental monitoring has been conducted for decades, comprising analyses of contaminants in mussel, fish and sediments. DDT was used as an insecticide in orchards surrounding the fjord between World War II and 1970. Since the early 1990 s, elevated concentrations of DDT were found in mussels and fish. Unexpectedly, DDT-concentrations increased towards present day, despite the discontinuation of use. The highest concentrations in mussels (in 2006) corresponded to about two orders of magnitude higher than background. Analyses of sediment core sections also indicated increased input towards present day. Shifts in climatic parameters, as well as increased amounts of soil dissolved organic carbon following a decline in atmospheric sulphate deposition may have contributed to this phenomenon. We warrant the need for increased knowledge of the effects of alterations in variables acting regionally and globally on the disposition of contaminants in ecosystems.

Development and testing of a prototype tool for integrated assessment of chemical status in marine environments

We report the development and application of a prototype tool for integrated assessment of chemical status in aquatic environments based on substance- and matrix-specific environmental assessment criteria (thresholds). The Chemical Status Assessment Tool (CHASE) integrates data on hazardous substances in water, sediments and biota as well as bio-effect indicators and is based on a substance- or bio-effect-specific calculation of a ‘contamination ratio’ being the ratio between an observed concentration and a threshold value. Values <1.0 indicate areas potentially ‘unaffected’, while values >1.0 indicate areas potentially ‘affected’. These ratios are combined within matrices, i.e. for water, sediment and biota and for biological effects. The overall assessment used a ‘one out, all out principle’ with regard to each matrix. The CHASE tool was tested in the Baltic Sea and the North Sea in 376 assessment units. In the former, the chemical status was >1.0 in practically all areas indicating that all areas assessed were potentially affected. The North Sea included areas classified as unaffected or affected. The CHASE tool can in combination with temporal trend assessments of individual substances be advantageous for use in remedial action plans and, in particular, for the science-based evaluation of the status and for determining which specific substances are responsible for a status as potentially affected.

Additive Models Reveal Sources of Metals and Organic Pollutants in Norwegian Marine Sediments

We characterized spatial patterns of surface sediment concentrations of seven polychlorinated biphenyls (PCBs), seven polycyclic aromatic hydrocarbons (PAHs), three chlorinated pesticides, and five metals in Norwegian waters and Skagerrak. In total, we analyzed 5036 concentrations of 22 chemical substances that were measured between 1986 and 2014 at 333 sampling sites by means of generalized additive models (GAMs). We found that GAMs with organic carbon content of the sediment and latitude and longitude as co-variates explained as ca. 75% of the variability of the contaminant sediment concentrations. For metals, a predominantly hotspot-driven spatial pattern was found, i.e., we identified historical pollution hotspots (e.g., Sørfjord in western Norway) for mercury, zinc, cadmium, and lead. Highest concentrations of PAHs and PCBs were found close to densely populated and industrialized regions, i.e., in the North Sea and in the Kattegat and Skagerrak. The spatial pattern of the PCBs suggests the secondary and diffuse atmospheric nature of their sources. Atmospheric inputs are the main sources of pollution for most organic chemicals considered, but north of the Arctic circle, we found that concentrations of PAHs increased from south to north most likely related to a combination of coal-eroding bedrock and the biological pump. The knowledge acquired in the present research is essential for developing effective remediation strategies that are consistent with international conventions on pollution control.

Temporal trends of persistent organic pollutants in Arctic marine and freshwater biota

More than 1000 time-series of persistent organic pollutants (POPs) in Arctic biota from marine and freshwater ecosystems some extending back to the beginning of 1980s were analyzed using a robust statistical method. The Arctic area encompassed extended from Alaska, USA in the west to northern Scandinavian in the east, with data gaps for Arctic Russia and Arctic Finland. The aim was to investigate whether temporal trends for different animal groups and matrices were consistent across a larger geographical area. In general, legacy POPs showed decreasing concentrations over the last two to three decades, which were most pronounced for α-HCH and least pronounced for HCB and β-HCH. Few time-series of legacy POPs showed increasing trends and only at sites suspected to be influenced by local source. The brominated flame retardant congener BDE-47 showed a typical trend of increasing concentration up to approximately the mid-2000s followed by a decreasing concentration. A similar trend was found for perfluorooctane sulfonic acid (PFOS). These trends are likely related to the relatively recent introduction of national and international controls of hexa- and hepta-BDE congeners and the voluntary phase-out of PFOS production in the USA in 2000. Hexabromocyclododecane (HBCDD) was the only compound in this study showing a consistent increasing trend. Only 12% of the long-term time-series were able to detect a 5% annual change with a statistical power of 80% at α < 0.05. The remaining 88% of time-series need additional years of data collection before fulfilling these statistical requirements. In the case of the organochlorine long-term time-series, 45% of these would require >20 years monitoring before this requirement would be fulfilled.

The Environmental Status of Norwegian Coastal Waters

Abstract Norway has a very long and varied coastline which includes archipelagos, beaches, mountains, and the famous fjords. Its length together with topography and, not least, the Gulf Stream govern its climate, which appears to be becoming both warmer and wetter. This precipitation results in large inputs of freshwater into the surrounding seas. Important habitats include kelp forests, seagrass beds, soft substrates, and the world’s largest cold-water corals reefs. The importance of the diversity and ecosystem function of these habitats is becoming increasingly understood although they are all challenged by anthropogenic pressures, not least climate change. Despite being relatively sparsely populated Norwegian cities and several major industries may exert considerable pressure on the marine environment. These include the oil and gas industry and aquaculture, both of which are extremely important to the Norwegian economy. In addition, capture fisheries remain important. Overall trends in the input of pollutants to coastal waters are largely downward (metals and organics), with a few exceptions, noticeably some increases in nutrients in the south due to changes in climate. A similar overall decline in pollutants in coastal marine organisms is also apparent, with a reduction in concentrations in recent years. Many contaminated sites persist however, including inner harbors and areas which have historically received industrial discharges. Consumption advices for seafood persist at many of these sites. There is also some evidence of increasing mercury concentrations in some areas, such as Oslofjord. Norway enforces most European and other international agreements regarding the protection and management of marine ecosystems, and has now has four national marine parks, all in the South of the country, as well as several other protected areas.

Mytilus spp. as sentinels for monitoring microplastic pollution in Norwegian coastal waters: A qualitative and quantitative study

Microplastic (MP) contamination is ubiquitous in the environment and many species worldwide have been shown to contain MP. The ecological impact of MP pollution is still unknown, thus there is an urgent need for more knowledge. One key task is to identify species suitable as sentinels for monitoring in key eco-compartments, such as coastal waters. In Norway, mussels (Mytilus spp.) have been monitored for hazardous contaminants through OSPAR since 1981. Norway has the longest coastline in Europe and adding MP to the Norwegian Mussel Watch is therefore important in a European and global context. The present study reports MP data in mussels (332 specimens) collected from multiple sites (n = 15) spanning the whole Norwegian coastline. MPs were detected at all locations, except at one site on the west coast. Among the most surprising findings, mussels from the Barents Sea coastline in the Finnmark region, contained significantly more MPs than mussels from most of the southern part of the country, despite the latter sites being located much closer to major urban areas. Only mussels from a site located very close to Oslo, the capital, contained levels similar to those observed in the remote site in Finnmark. In total an average of 1.5 (±2.3) particles ind-1 and 0.97 (±2.61) particles w.w. g-1 was found. The most common MPs were <1 mm in size, and fibres accounted for 83% of particles identified, although there was inter-site variability. Thirteen different polymeric groups were identified; cellulosic being the most common and black rubbery particles being the second. This study suggests Mytilus spp. are suitable for semi-quantitative and qualitatively monitoring of MPs in coastal waters. However, some uncertainties remain including mussel size as a confounding factor that may influence ingestion, the role of depuration and other fate related processes, and this call for further research.