O. A. Ozen

The effects of topical treatment with curcumin on burn wound healing in rats

The present study was designed to determine the role of topical treatment with curcumin (Cur) on burn wound healing in rats. The Wistar-albino rats were randomly allotted into one of three experimental groups: 4th, 8th and 12th day (post burn) and all groups include subgroups which Burn and Burnxa0+xa0Cur. Each group contains 12 animals. Burn wounds were made on the back of rat and Cur was administered topically. At the end of the study, all animals were sacrificed and the wound tissues removed for analyse to biochemical and histopathological changes. There was a significant increase in the hydroxyproline levels in the skin of the Cur groups. Cur treated wounds were found to heal much faster as indicated by improved rates of inflammatory cells, collagen deposition, angiogenesis, granulation tissue formation and epithelialization which were also confirmed by histopathological and biochemical examinations. Our data also indicate that there is a rise in the expression of proliferating cell nuclear antigen in skin tissues of Cur-treated rats in the Burn group. The results clearly substantiate the beneficial effects of the topical application of Cur in the acceleration of wound healing.

Effects of quercetin and fish n-3 fatty acids on testicular injury induced by ethanol in rats

The aim of this study was to investigate the effects of quercetin and fish n‐3 fatty acids on the changes in testis induced by ethanol. Forty‐five rats divided into five groups, control, ethanol, ethanol+quercetin, ethanol+fish n‐3 fatty acids and ethanol+quercetin+fish n‐3 fatty acids. At the end of 8 weeks, all the rats were sacrificed. Degenerative changes in histopathological analyses, the decreased body weight gain and seminiferous tubule diameters in ethanol group have been observed. TUNEL assay also showed an increase in apoptotic cell number. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH‐Px), xanthine oxidase (XO) and testosterone levels were decreased as well as the levels of malondialdehyde (MDA) and nitric oxide (NO) were increased in ethanol group. Histopathological changes caused by ethanol have been improved by quercetin and fish n‐3 fatty acids. It was also found that protection was provided by increasing SOD, CAT and GSH‐Px activities in groups administered quercetin, fish n‐3 fatty acids and quercetin+fish n‐3 fatty acids, and by decreasing the levels of MDA and NO in groups administered both quercetin and fish n‐3 fatty acids together. These results suggest that quercetin and fish n‐3 fatty acids are beneficial agents to reduce testicular injury induced by ethanol except for testosterone levels.

Protective effects of fish omega‐3 fatty acids on doxorubicin‐induced testicular apoptosis and oxidative damage in rats

The aim of this study was to examine the protective effects of fish omega‐3 (n‐3) fatty acids on acute doxorubicin (DOX)‐induced testicular apoptosis and oxidative damage. 24 male rats were divided into three groups: control, DOX‐treated and DOX+fish n‐3 fatty acids. Fish n‐3 fatty acids (400 mg kg−1) were given for 30 days by intragastric gavage. The rats received a single intraperitoneal injection of DOX (30 mg kg−1) and were sacrificed after 48 h. The DOX+fish n‐3 fatty acids group showed a decrease in malondialdehyde levels and increased activities of superoxide dismutase and glutathione peroxidase in comparison with the DOX‐treated group. Acute DOX treatment caused severe damage such as disorganisation and separation of germ cells. The fish n‐3 fatty acids‐pretreated rats showed an improved histological appearance in the DOX‐treated group. Our data indicate a reduction in the activity of terminal deoxynucleotidyl transferase mediated dUTP nick end labelling; there was a rise in the expression of proliferating cell nuclear antigen in testis tissues of the DOX+fish n‐3 fatty acids group compared with DOX‐treated group. These data suggested that fish n‐3 fatty acids pre‐treatment may be beneficial for spermatogenesis following acute DOX‐induced testicular damage by decreasing germ cell apoptosis and oxidative stress.

Protective effects of melatonin against arsenic-induced apoptosis and oxidative stress in rat testes

This study aimed to investigate the protective effects of melatonin against arsenic-induced apoptosis and oxidative stress in rat testes. A total of 27 male rats were divided into 3 groups: control (saline: 5 ml kg−1 day−1, intragastrically), arsenic (sodium arsenite (NaAsO2): 5 mg kg−1 day−1, intragastrically), and arsenic + melatonin (sodium arsenite (NaAsO2): 5 mg kg−1 day−1, intragastrically and melatonin: 25 mg kg−1 day−1, intraperitoneally) group. At the end of 30 days, the rats were killed under anesthesia. Histopathological examination showed that testicular injury mediated by arsenic was ameliorated by the administration of melatonin. The number of apoptotic germ cell was increased, and the number of proliferating cell nuclear antigen (PCNA)-positive germ cell was decreased in testis after arsenic administration. Our data indicate a significant reduction in the activity of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling, and there was a rise in the expression of PCNA in testis of arsenic + melatonin group. The decreased superoxide dismutase, catalase, and glutathione peroxidase activities as well as increased malondialdehyde levels in testis due to arsenic administration were also counteracted by melatonin. These data suggested that melatonin has beneficial effects against arsenic-induced testicular damage by decreasing morphological damage, germ cell apoptosis, lipid peroxidation, and oxidative stress. Our results suggest that melatonin plays a protective role against arsenic-induced testicular apoptosis and oxidative stress.

Protective effects of quercetin against arsenic-induced testicular damage in rats.

This study investigated the effect of quercetin on changes in testes due to arsenic exposure. Twenty‐seven male rats were divided into three groups: control (10 ml kg−1 day−1 saline), arsenic (10 mg kg−1 day−1 sodium arsenite) and arsenic + quercetin (arsenic + 50 mg kg−1 day−1 quercetin). The rats were sacrificed at the end of 15‐day experiment. There was no difference between control group and arsenic group in body weight gain, testicular weight and serum total testosterone level. Quercetin treatment did not cause a significant difference in these parameters. In the arsenic group rats, we determined deterioration in the structure of seminiferous tubules, a decrease in the number of spermatogenic cells, an increase in the number of apoptotic cells, a decrease in the number of PCNA‐positive cells, a decrease in SOD, CAT and GSH‐Px activities, and an increase in the MDA level in testicular tissue. In all these changes, arsenic+quercetin group showed an improved compared to arsenic group. The amount of arsenic increased in the arsenic group was compared to the control group, and there was no difference between arsenic group and arsenic + quercetin group in the amount of arsenic. In conclusion, quercetin prevented arsenic‐induced testicular damage with its anti‐apoptotic and antioxidant effects.

Neuroprotective effect of ebselen against intracerebroventricular streptozotocin-induced neuronal apoptosis and oxidative stress in rats

The goal of this study was to examine the neuroprotective effect of ebselen against intracerebroventricular streptozotocin (ICV-STZ)-induced oxidative stress and neuronal apoptosis in rat brain. A total of 30 adult male Sprague-Dawley rats were randomly divided into 3 groups of 10 animals each: control, ICV-STZ, and ICV-STZ treated with ebselen. The ICV-STZ group rats were injected bilaterally with ICV-STZ (3 mg/kg) on days 1 and 3, and ebselen (10 mg/kg/day) was administered for 14 days starting from 1st day of ICV-STZ injection to day 14. Rats were killed at the end of the study and brain tissues were removed for biochemical and histopathological investigation. Our results demonstrated, for the first time, the neuroprotective effect of ebselen on Alzheimer’s disease (AD) model in rats. Our present study, in ICV-STZ group, showed significant increase in tissue malondialdehyde levels and significant decrease in enzymatic antioxidants superoxide dismutase and glutathione peroxidase in the frontal cortex tissue. The histopathological studies in the brain of rats also supported that ebselen markedly reduced the ICV-STZ-induced histopathological changes and well preserved the normal histological architecture of the frontal cortex tissue. The number of apoptotic neurons was increased in frontal cortex tissue after ICV-STZ administration. Treatment of ebselen markedly reduced the number of degenerating apoptotic neurons. The study demonstrates the effectiveness of ebselen, as a powerful antioxidant, in preventing the oxidative damage and morphological changes caused by ICV-STZ in rats. Thus, ebselen may have a therapeutic value for the treatment of AD.

Cardioprotective effects of fish omega-3 fatty acids on doxorubicin-induced cardiotoxicity in rats

The aim of this study was to investigate the protective effects of fish omega-3 (n-3) fatty acids on doxorubicin (DOX)-induced acute cardiotoxicity. A total of 24 rats were divided into three groups: control, DOX-treated, and DOX treated with fish n-3 fatty acids. Control group received 0.4 ml/kg/day of saline intragastrically. The rats in the fish n-3 fatty acid-pretreated group were given 400 mg/kg/day fish n-3 fatty acids for 30 days by intragastric intubation. To induce acute cardiotoxicity, DOX (30 mg/kg) was injected intraperitoneally by a single dose and the rats were killed after 48 h. DOX treatment caused severe damage in heart tissues. Disorganization of myocardial muscle fibers, myofibrillar loss, and cardiotoxic myocardial fibers with cytoplasmic vacuoles were seen. Fish n-3 fatty acid-treated rats showed an improved histological appearance in the DOX-treated group. Our data indicate a significant reduction in the activity of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling in cardiomyocytes of the DOX-treated group with fish n-3 fatty acids therapy. The DOX-treated with fish n-3 fatty acids group showed a significant decrease in malondialdehyde levels, and an increase in superoxide dismutase and glutathione peroxidase activities in comparison with the DOX-treated group. This study showed that fish n-3 fatty acids may be a suitable cardioprotector against acute toxic effects of DOX.

Determination of normal splenic volume in relation to age, gender and body habitus: a stereological study on computed tomography

BACKGROUNDnThe aim of this study is to assess and document the dimensions of the normal spleen measured on computed tomography (CT) images with the normal splenic volume measured by Cavalieri principle on CT images and thereby serve as a baseline for comparison in cases of splenomegaly using abdominal CT. To investigate the relationship between these changes and body mass index, gender, abdominal diameters.nnnMATERIALS AND METHODSnWe retrospectively reviewed abdominal CT examinations of 212 adults between the ages of 20 and 88 years. There were seven groups of patients. The spleen volume (SV) measurements using abdominal CT images of each patient on the Image Information Systems were performed with Cavalieri principle.nnnRESULTSnThe mean SV and splenic length (SL), width (SW), and thickness (ST) for the total study population of 212 patients was 198 ± 88 cm³, 9.96 ± 2.1 cm, 8.87 ±± 1.6 cm and 4.58 ± 0.8 cm, respectively. There was a strong correlation between SV and ST (r = 0.752, p < 0.001), SL (r = 0.735, p < 0.001), SW (r = 0.681,p < 0.001) mean values of total study population. Comparison between mean splenic dimension parameters for males and females showed a statistically significant difference (p = 0.032 for SV, p = 0.04 for ST) but no statistically significant difference with SL and SW. Also there was a positive correlation between SV and body height, sagittal abdominal diameter and transvers abdominal diameter in mean of total groups and female groups, there was no correlation in males.nnnCONCLUSIONSnThe normal reference ranges for SV and size given in this study canserve as a standard to judge whether splenomegaly is present in patients.

Effects of Formaldehyde Inhalation on Zinc, Copper and Iron Concentrations in Liver and Kidney of Male Rats

In the present study, adult Wistar albino male rats were exposed to formaldehyde at different periods (subacute and subchronic) and concentrations (5.0 and 10.0xa0ppm) in order to figure out the changes in the concentration of Zn, Cu and Fe. It was observed that the formaldehyde inhalation caused gradual decline of body weights in the experimental groups when compared with control groups. It was found that subacute (4-week) or subchronic (13-week) exposure to formaldehyde for rats may cause growth retardation. After inhalation procedure, concentration of copper, zinc and iron were determined in liver and kidney tissues of rats using atomic absorption spectrophotometer. In addition, concentrations of Cu, Zn and Fe changed by the effect of formaldehyde in subacute and subchronic groups.

Answer to the Letter to the Editor concerning ‘Effects of quercetin and fish n‐3 fatty acids on testicular injury induced by ethanol in rats’

Dear editor, We appreciate the interest and comments by Eid and colleagues concerning our article entitled ”Effects of quercetin and fish n-3 fatty acids on testicular injury induced by ethanol in rats” Figures 1 panel c and 2 panel c were accidentally mistaken in the image editing stage. This has been corrected in an erratum notice. In the legend of fig. 1, we mentioned that S is abbreviation of Sertoli cells and V indicates vacuole. Even if these abbreviations are not clear in images of fig. 1, these could be recognised in colour images. We think that Leydig cell atrophy is correlated with decreased testosterone levels in the ethanol group. In our study, a significant decrease was observed in the serum testosterone levels of the ethanol group in comparison with the control group. In our study, the numbers of apoptotic cells were increased in the ethanol group. We think that the cause of vacuoles in the seminiferous tubules of the ethanol group is loss of germ cell resulting from apoptosis and vacuolisation in Sertoli cells of the ethanol group. Sertoli cells are the primary cellular target of ethanol toxicity. Ethanol administration leads to structural damage in Sertoli cells. Sertoli cell vacuolisation is a marker of ethanolinduced testicular damage. Additionally, we found that nitric oxide (NO) levels increased in the ethanol group compared with the control group. These increased NO levels may induce germ cell apoptosis. Furthermore, NO has an inhibitory effect on testosterone release. According to our results, increased NO levels were shown to be closely associated with oxidative stress, apoptosis of germ cells and decreased testosterone levels. In our research laboratory, we do not have any opportunities to observe in electron microscopy and iNOS staining for further analyses. We are planning to cite Eid and collaborators’ studies in our new ethanol studies.