O.-J. Kwon

MICB polymorphisms and haplotypes with MICA and HLA alleles in Koreans

Major histocompatibility complex (MHC) class I chain-related gene B (MICB) is located within the human MHC class I region. The location of MICB in the MHC region may imply the presence of linkage disequilibrium with polymorphic MICA and human leukocyte antigen (HLA) loci. MICB is also polymorphic; however, MICB polymorphisms have not been investigated in Koreans. Using sequence-based typing (SBT), we estimated the allelic frequencies of MICB and haplotypes with MICA, HLA-B, and HLA-DRB1 at high resolution in a population of 139 unrelated Korean individuals. Eight MICB alleles were identified. The most frequent allele was MICB*005:02/*010 (57.2%), followed by *002 (11.5%), *004 (8.3%), *005:03 (8.3%), and *008 (6.8%). The most common two-locus haplotypes were MICB*005:02/*010-MICA*010 (19.4%), MICB*005:02/*010-DRB1*15:01 (6.5%), and MICB*005:02/*010-B*15:01 (10.4%); the most common three-locus haplotypes were B*15:01-MICA*010-MICB*005:02/*010 (5.8%) and MICA*010-MICB*005:02/*010-DRB1*04:06 (10.4%); and the most common four-locus haplotype was B*15:01-MICA*010-MICB*005:02/*010-DRB1*04:06 (5.8%). This is the first study to provide information about MICB allele frequencies and haplotypes with HLA in Koreans. These study results should help understand mechanisms of disease association between the MICB locus and neighboring loci in Koreans.

HLA-A*02:07:02, a new allele identified by sequence-based typing in a Korean individual

The new allele A*02:07:02 shows a single nucleotide substitution compared with A*02:07:01 at codon 233 (ACC → ACT) without any amino acid change.

Novel HLA-Cw*01 allele, Cw*010203, identified by sequence-based typing*

In this report, we describe the identification of an human leucocyte antigen-Cw*0102 (HLA-Cw*0102) nucleotide sequence variant, a new HLA-Cw*010203, in a case - control study by using sequence-based typing. Allele HLA-Cw*010203 showed one nucleotide difference with HLA-Cw*010201 by a silent substitution at codon 130 (CTG-->CTA).

A new HLA-A*02 allele, A*02:465

The new allele A*02:465 showed one nucleotide difference with A*02:06:01 (172G>A).

Identification of a novelHLA-A*02allele,A*02:428, by sequence-based typing: NEW ALLELE Alerts

HLA-A*02:428 differs from A*02:06:01 by a non-synonymous mutation at codon 260 (CAT to GAT) in exon 4.

A new HLA‐B*15 allele, HLA‐B*15:263, identified in a Korean individual

HLA-B*15:263 differs from HLA-B*15:18:01 by a single nucleotide exchange at position 824, C>G (codon 251 TCT>TGT).

A new HLA-C*07:244 allele identified by sequence-based typing in a Korean individual.

The C*07:244 changes single nucleotide of C*07:02:01 at codon 75 (CGA → CAA), Arg to Gln.

A*11:164, a novel HLA‐A*11 allele identified by sequence‐based typing

The new allele A*11:164 showed one nucleotide difference with A*11:01:01 at codon 151 (CAT>CCT).

Sequencing of a new HLA-A allele: HLA-A*11:01:54

The new allele A*11:01:54 shows one nucleotide difference from A*11:01:01 at codon 35 (CGG/AGG).

A new HLA-C allele, C*06:99, identified by sequence-based typing in a Korean individual

The C*06:99 allele substitutes one nucleotide of C*06:02:01:01 at codon 50 (CCG→CTG), Pro to Leu.