Oana Teodora Moldovan

An early modern human from Romania with a recent Neanderthal ancestor

Neanderthals are thought to have disappeared in Europe approximately 39,000–41,000 years ago but they have contributed 1–3% of the DNA of present-day people in Eurasia. Here we analyse DNA from a 37,000–42,000-year-old modern human from Peştera cu Oase, Romania. Although the specimen contains small amounts of human DNA, we use an enrichment strategy to isolate sites that are informative about its relationship to Neanderthals and present-day humans. We find that on the order of 6–9% of the genome of the Oase individual is derived from Neanderthals, more than any other modern human sequenced to date. Three chromosomal segments of Neanderthal ancestry are over 50 centimorgans in size, indicating that this individual had a Neanderthal ancestor as recently as four to six generations back. However, the Oase individual does not share more alleles with later Europeans than with East Asians, suggesting that the Oase population did not contribute substantially to later humans in Europe.

The genetic history of Ice Age Europe

Modern humans arrived in Europe ~45,000 years ago, but little is known about their genetic composition before the start of farming ~8,500 years ago. We analyze genome-wide data from 51 Eurasians from ~45,000-7,000 years ago. Over this time, the proportion of Neanderthal DNA decreased from 3–6% to around 2%, consistent with natural selection against Neanderthal variants in modern humans. Whereas the earliest modern humans in Europe did not contribute substantially to present-day Europeans, all individuals between ~37,000 and ~14,000 years ago descended from a single founder population which forms part of the ancestry of present-day Europeans. A ~35,000 year old individual from northwest Europe represents an early branch of this founder population which was then displaced across a broad region, before reappearing in southwest Europe during the Ice Age ~19,000 years ago. During the major warming period after ~14,000 years ago, a new genetic component related to present-day Near Easterners appears in Europe. These results document how population turnover and migration have been recurring themes of European pre-history.

Peştera cu Oase 2 and the cranial morphology of early modern Europeans

Between 2003 and 2005, the Peştera cu Oase, Romania yielded a largely complete early modern human cranium, Oase 2, scattered on the surface of a Late Pleistocene hydraulically displaced bone bed containing principally the remains of Ursus spelaeus. Multiple lines of evidence indicate an age of ≈40.5 thousand calendar years before the present (≈35 ka 14C B.P.). Morphological comparison of the adolescent Oase 2 cranium to relevant Late Pleistocene human samples documents a suite of derived modern human and/or non-Neandertal features, including absence of a supraorbital torus, subrectangular orbits, prominent canine fossae, narrow nasal aperture, level nasal floor, angled and anteriorly oriented zygomatic bones, a high neurocranium with prominent parietal bosses and marked sagittal parietal curvature, superiorly positioned temporal zygomatic root, vertical auditory porous, laterally bulbous mastoid processes, superiorly positioned posterior semicircular canal, absence of a nuchal torus and a suprainiac fossa, and a small occipital bun. However, these features are associated with an exceptionally flat frontal arc, a moderately large juxtamastoid eminence, extremely large molars that become progressively larger distally, complex occlusal morphology of the upper third molar, and relatively anteriorly positioned zygomatic arches. Moreover, the featureless occipital region and small mastoid process are at variance with the large facial skeleton and dentition. This unusual mosaic in Oase 2, some of which is paralleled in the Oase 1 mandible, indicates both complex population dynamics as modern humans dispersed into Europe and significant ongoing human evolution once modern humans were established within Europe.

Can Environment Predict Cryptic Diversity? The Case of Niphargus Inhabiting Western Carpathian Groundwater

In the last decade, several studies have shown that subterranean aquatic habitats harbor cryptic species with restricted geographic ranges, frequently occurring as isolated populations. Previous studies on aquatic subterranean species have implied that habitat heterogeneity can promote speciation and that speciation events can be predicted from species’ distributions. We tested the prediction that species distributed across different drainage systems and karst sectors comprise sets of distinct species. Amphipods from the genus Niphargus from 11 caves distributed along the Western Carpathians (Romania) were investigated using three independent molecular markers (COI, H3 and 28S). The results showed that: 1) the studied populations belong to eight different species that derive from two phylogenetically unrelated Niphargus clades; 2) narrow endemic species in fact comprise complexes of morphologically similar species that are indistinguishable without using a molecular approach. The concept of monophyly, concordance between mitochondrial and nuclear DNA, and the value of patristic distances were used as species delimitation criteria. The concept of cryptic species is discussed within the framework of the present work and the contribution of these species to regional biodiversity is also addressed.

Conservation of biodiversity in Romania.

This paper briefly discusses the history and development of nature protection in Romania. It summarises the current situation of protected areas, and discusses the ecological, ethical and philosophical ideas concerning biodiversity conservation in the country.

Bacterial and Fungal Diversity of Quaternary Cave Sediment Deposits

The bacterial and fungal assemblages of clastic sediments collected from two caves located in north-western Romania were investigated by assessing ITS and 16S rRNA gene diversity. Bacterial members belonging to Chloroflexi, Nitrospirae, Proteobacteria, Firmicutes, Acidobacteria, Gemmatimonadetes, and fungal members of Ascomycota were identified. Except for Bacillus sp., all bacteria were related to uncultured or unknown species and the majority (86%) of the bacterial sequences from one of the caves had no close GenBank relatives. The bacterial sequences obtained clustered with species found in extreme environments. Half of the bacterial operational taxonomic units were clustered with clones isolated from deep subsurface sediments of a radioactively contaminated site in the USA. The present study represents the first attempt to identify microorganisms in Quaternary cave sediments. Supplemental materials are available for this article. Go to the publishers online edition of Geomicrobiology Journal to view the supplemental file.

Ancient human footprints in Ciur-Izbuc Cave, Romania

In 1965, Ciur-Izbuc Cave in the Carpathian Mountains of Romania was discovered to contain about 400 ancient human footprints. At that time, researchers interpreted the footprints to be those of a man, woman and child who entered the cave by an opening which is now blocked but which was usable in antiquity. The age of the prints (≈10-15 ka BP) was based partly on their association with cave bear (Ursus spelaeus) footprints and bones, and the belief that cave bears became extinct near the end of the last ice age. Since their discovery, the human and bear evidence and the cave itself have attracted spelunkers and other tourists, with the result that the ancient footprints are in danger of destruction by modern humans. In an effort to conserve the footprints and information about them and to reanalyze them with modern techiques, Ciur-Izbuc Cave was restudied in summer of 2012. Modern results are based on fewer than 25% of the originally described human footprints, the rest having been destroyed. It is impossible to confirm some of the original conclusions. The footprints do not cluster about three different sizes, and the number of individuals is estimated to be six or seven. Two cases of bears apparently overprinting humans help establish antiquity, and C-14 dates suggest a much greater age than originally thought. Unfortunately, insufficient footprints remain to measure movement variables such as stride length. However, detailed three-dimensional mapping of the footprints does allow a more precise description of human movements within the cave.

The relationships between land cover, climate and cave copepod spatial distribution and suitability along the Carpathians

The distribution of subterranean copepods may reflect the persistence of cave assemblages in relation to the environmental health of the overlying landscape. Areas supporting groundwater fauna were established by modelling the persistence of seven copepod species using a geographical information system (GIS). Environmental drivers were found to influence subterranean copepod distribution in the caves of the Romanian Carpathians. Habitat-based modelling, using ordinary least squares regression and geographically-weighted regression to identify the significant predictors explaining copepod habitat suitability, predicted suitable areas for the selected taxa. The most constant predictor was land cover, a measure of human impact and climate change, followed by precipitation and altitude. The model performed well for the majority of analysed taxa, and the areas predicted as suitable for narrowly distributed taxa overlapped with observed distributions. GIS facilitated the prediction of suitable habitat, and also enabled spatial autocorrelation to be tested. The results of this study demonstrate the importance of sustainable management of the terrestrial surface in limestone areas in conserving copepod biodiversity.

Temporal variability of fauna and the importance of sampling frequency in the hyporheic zone

Sampling of the hyporheic zone along a polluted stretch of the Arieş River (Romania) showed contrasting biodiversity and abundance patterns at different time scales. The species considered in this study were Crustacea and Oligochaeta and only the more frequently occurring species were included in the analyses. Monthly composition of these species showed a little correlation with physical and chemical parameters. In contrast, when subsets of the monthly samples of the two most abundant species, Microcharon sp. and Diacyclops languidoides, were analyzed, there were strong correlations with at least some of the major physical and chemical parameters (temperature, flow rate, Al, and Fe) in each of the three possible seasonal series. However, correlations between seasonal series were sometimes contradictory and appeared to be artifacts related to sparse data. It is suggested that monthly or more frequent sampling is required for the complete assessment of biodiversity and processes in the hyporheic zone and to study the relationship between the hyporheos and the surface river ecosystem.

Caves and karst of Ecuador – state-of-the-art and research perspectives

Abstract Current knowledge of caves and karst regions of Ecuador is scarce and broadly limited to discrete areas such as that of the Galápagos volcanokarst. In continental Ecuador, carbonate karst mostly outcrops in the Amazonian basin and accounts for 5–10% of the surface of the country. However, owing to the difficulties of access within the Amazonian rainforest, most surface and subterranean karst is yet to be revealed. In this review, we present an updated map of the solutional karst of Ecuador based on the most recent geological surveys and our own research. We describe the principal karst regions of Ecuador from the Amazonian basin (Napo and Santiago) as well as the Galápagos pseudokarst. We show that Ecuador karst research may be of considerable importance for both basic and applied research owing to its geographical position and intrinsic vulnerabilities. We discuss the main challenges of karst-related research in Ecuador, such as paleoclimatic studies, subterranean biodiversity, and archeology. We discuss the main vulnerabilities and hazards related to karst uses in Ecuador, considering the paramount importance of tourism for the country.