Obtułowicz K

Contact hypersensitivity and allergic contact dermatitis among school children and teenagers with eczema

Background:  Patch testing is an essential procedure in the investigation of eczema in children.

The most important contact sensitizers in Polish children and adolescents with atopy and chronic recurrent eczema as detected with the extended European Baseline Series

To cite this article: Czarnobilska E, Obtulowicz K, Dyga W, Spiewak R. The most important contact sensitizers in Polish children and adolescents with atopy and chronic recurrent eczema as detected with the extended European Baseline Series. Pediatr Allergy Immunol 2011; 22:252–256.

A half of schoolchildren with 'ISAAC eczema' are ill with allergic contact dermatitis

Background  Similarity in clinical symptoms between atopic eczema (AE) and allergic contact dermatitis (ACD) may lead to misdiagnoses in both clinical practice and epidemiological studies. As patch testing for contact allergy does not seem popular among paediatric allergists, the resulting bias leads mainly to under diagnosing of ACD and over diagnosing of AE in children and adolescents.

Antibody reactivity in patients with IgE-mediated wheat allergy to various subunits and fractions of gluten and non-gluten proteins from ω-gliadin-free wheat genotypes

[b]Abstract Introduction and objective[/b]. Gluten proteins (gliadins and glutenins) are polymorphic wheat storage proteins of allergenic properties. Significant differences in chemical composition between both protein groups allow to expect highly specific immunological response of individual subunits and fractions in reactions with IgE sera of people allergic to wheat. The aim of these studies was to identify and characterize the most allergenic gluten proteins (GP) and nongluten proteins (NGP) occurred in two closely related wheat hybrid genotypes. [b]Materials and method.[/b] 3xC and 3xN wheat hybrids, which differ strongly in regard of gliadin composition, were analyzed. Seven people manifesting different symptoms of wheat allergy donated sera for the experiment. The technique of immunoblotting after SDS-PAGE was used for identification of allergenic subunits and fractions among GP and NGP. Immunologically active protein bands were visualized by chemiluminescence. [b]Results[/b]. Great variation of immunodetection spectra was observed. Results of immunoblotting showed LMW glutenins to be of highest, gliadins of medium, while NGP of lowest allergenicity for selected patients. The 43-kDa and 47-kDa LMW glutenin subunits, 40-kDa and 43-kDa γ-gliadin fractions and 49-kDa NGP can be considered as the most immunoreactive among all protein bands [b]separated by SDS-PAGE. CONCLUSION [/b] The observed differentiation of immunodetection spectra allows to model highly specific IgE-binding profiles of allergenic wheat proteins attributed to individual patients with symptoms of gluten intolerance. Highly immunoreactive subunits and fractions among GP and NGP were identified. The observed immunoreactivity of 49 kDa NGP is worth to emphasize, as it has never been reported as wheat allergenic protein before.

Corrigendum to "Nickel allergy and relationship with Staphylococcus aureus in atopic dermatitis" [J. Trace Elem. Med. Biol. 33 (2016) 1-7].

Department of Clinical and Environmental Allergology, Jagiellonian University Medical College, 31-531 Krakow, Poland Department of Dermatology, Jagiellonian University Medical College, 31-066 Krakow, Poland Centre for Microbiological Research and Autovaccines Ltd., 31-016 Krakow, Poland University of Copenhagen Research Centre for Control of Antibiotics Resistance-UC-Care Faculty of Health and Medical Sciences, University of openhagen, Stigbøjlen 4, 1870 Frb. C., 1-20 Frb. C., Copenhagen, Denmark

Influence of Nickel on Reactivity of Dermatophagoides pteronyssinus and Staphylococcus aureus Antigens in Atopic Dermatitis

All of these eyes were eviscerated under general anesthesia using an evisceration spoon to separate the uveal tissue from the scleral shell. The delivery of the globe contents was assisted by suction machine. In the cases that followed, removal of contents was facilitated by injecting saline in the suprachoroidal space using a blunt cannula to induce choroidal detachment The inside of the globe was then cleaned with a gauze swab making sure that no uveal remnants are left [4].

The Estimation of Reliability in the Preventive Examination of Allergic Diseases with Knowledge Discovery Methods

The paper concerns the problem of analyzing the reliability of preventive examination of allergic diseases. The authors propose an approach based on the employment knowledge discovery methods to examine data obtained from a questionnaire and tests. As a result of the examination, the association rule model is built. The model describes the relations between the survey answers and the medical test results. The evaluation of the relations provides the answers for the quality and the accuracy of the survey. The Predictive Model Markup Language (PMML) and XQuery language are involved to facilitate an environment for the systematic examination of complex mining models. In the final part of the paper, the preliminary results of experiments are presented.