Olga Grygorieva

Biological properties of black mulberry-derived food products ( Morus nigra L.)

BACKGROUND: Black mulberry is a good source of bioactive compounds and especially of anthocyanin. OBJECTIVES: The objective of this study was to evaluate the morphological, biochemical, technological and antioxidant properties of black mulberry fruit and fruit-derived food products. MATERIALS AND METHODS: Total anthocyanin content, total sugar content, ascorbic acid content, pH and antioxidant activity was analyzed in black mulberry juice, jam, jelly, syrup, liqueur, compote, wine and cake. All products were produced from selected genotypes of black mulberry grown in Slovakia. RESULTS: Reducing sugar content ranged from 6.50 mg.kg–1 FM in wine to 60.01 mg.kg–1 FM in jam, ascorbic acid content was from 0.27 mg.100 g–1 FM in wine to 1.10 mg.100 g–1 FM in fruits conserved in honey without sterilization. Content of anthocyanin varied from 21.4 in wine to 106.4 mg.dm–3 in fresh juice. Detected pH values ranged from 3.28 in jelly to 3.69 in liqueur. All tested samples had strong DPPH• radical scavenging activity with the best values in black mulberry compote with honey – 74.02% and jam 71.22% FM. Differences between the genotypes were significant and the content of the anthocyanin was significantly higher in genotypes with lower fruit weight (P ≤ 0.05). CONCLUSION: The results showed that black mulberry fruits and derived products are rich in biologically active compounds and can be used in food industry and human nutrition.

Identification of sweet chesnut pollen in bee pollen pellet using using molecular analysis.

Castanea sativa posses many characteristics that are used by human for different purposes, not only as a part of the food. One of them is the utilization of the sweet chesnut pollen for its pharmacological benefits. Actually, no information about the DNA based identification of the sweet chesnut exist. Here, an identification of Castanea sativa based on the specific DNA fragment amplification is described for the first time. Sweet chesnut identification was performed in the very complex sample of bee pollen pellets that were identified as to contain sweet chesnut pollen grains by morphological analysis. First, bioinformatic analysis was performed to find a Castanea sativa conservative part of galactol synthase gene. BLAST alignment of the CDS of GolS1 gene was performed by BLASTtn against plants nucleotide sequences in the NCBI database to ensure for the specifity or existing nucleotide differences. Then, specific primers were subsequently designed and PCR amplification was performed. All the PCRs have run in duplicates for pollen pellet sample and two independent samples of Castanea sativa pure pollen. Restriction cleavage of the PCR amplified fragment was performed to confirm the specifity of the obtained PCR product with the positive confirmation as the predicted three restriction fragments were obtained that fully correspond by the length to those from virtual clevage. Restriction endonuclease Hpy166II was used in restriction cleavage analysis. Castanea sativa pollen grains were confirmed reliable in multifloral pollen pellet by PCR and this approach has the potential to be used effectively for the authentication purposes of sweet chesnut.

Variation of fruits morphometric parameters of Elaeagnus multiflora thunb. germplasm collection

The aim of this study was to determine morphometric parameters of fruits within some genotypes of Elaeagnus multiflora Thunb., which are growing in the Forest-Steppe of Ukraine in M.M. Gryshko National Botanical Garden of NAS of Ukraine (NBG). Their morphometric parameters were following: fruit weight from 0.32 to 1.89 g, fruit length from 7.60 to 19.54 mm, fruit diameter from 4.39 to 10.32 mm, seed weight from 0.10 to 0.41 g, seed length from 7.40 to 13.30 mm, seed diameter from 1.34 to 5.07 mm. The shape indexes of fruits and seeds were found ranging from 1.25 to 1.56 and from 2.90 to 4.04, respectively. The analysis of coefficient of variation showed the difference of variability in morphometric characteristics between Elaeagnus multiflora samples. Data showed that the most variability of important selection characteristics is the average cumulative seeds weight - from 18.72 to 36.61%, seeds diameter - from 10.46 to 24.29%, fruits weight - from 9.15 to 22.24%. The other characteristics are more or less stable. Collected quantitative data were subjected to principal hierarchical cluster analysis. The cluster analysis of morphometric parameters exhibited that the Elaeagnus multiflora genotypes fell into two clusters. These cluster analysis results suggest that broad morphologic diversity was found in Elaeagnus multiflora genotypes examined in this study. The introduction population of the Elaeagnus multiflora , was created in the M.M. Gryshko National Botanical Garden in Kyiv, has a sufficient potential for successful selection work. These preliminary results could open the interest of farmers in this Elaeagnus multiflora and will be precedent for future domestication and introduction of the species in the agroproductive system in Ukraine and the rest of the world.

Green Barley as an Ingredient in Pasta: Antioxidant Activity and Sensory Characteristics Evaluation

Summary The aims of the present study was to determine antioxidant activity, sensory properties as well as total polyphenol, flavonoid and chlorophyll content of raw and cooked pasta enriched by 1%, 3% and 5% addition of green barley powder. Results of antioxidant activity showed that increase of green barley addition increase antioxidant activity of pasta. The values in raw pasta obtained by DPPH ranged from 1.17 (control) to 1.81 (5% addition) mg TEAC/g DM, after cooking values ranged from 0.82 (control) to 1.59 (5% addition) mg TEAC/g DM. Similar tendency was signed by molybdenum reducing antioxidant power method. In enriched pasta was also found higher content of total polyphenol (0.19 in control raw sample and 1.81 mg GAE/g DM in pasta with 5 % of green barley addition; values of cooked pasta ranged from 0.10 in control sample to 0.73 mg GAE/g DM in pasta with 5 % of green barley addition) as well as total flavonoid content (results of raw pasta were from 0.00 in control sample to 0.41 mg QE/g DM in pasta with 5% of green barley addition; values of cooked pasta were from 0.00 in control sample to 0.29 mg QE/g DM in variant with 5% green barley). Green barley also enriched pasta for chlorophyll with the best results in 5% addition. From prepared variants of enriched pasta the best overall acceptability was sign in 3% of green barley addition. Pastas enriched with plant-derived bioactive compounds such as green barley may confer health benefits to consumers.

Approaches to the determination of antioxidant activity of extracts from bee bread and safflower leaves and flowers

The main objective of this study was to develop approaches for the determination of total antioxidant activity of natural products (bee bread and safflower extracts) using DPPH radical scavenging assay. Considering that analytical procedures and results related to this assay and reported by many authors are significally differed between each other and depend on many factors (the nature of tested extracts, the nature of solvents for extraction, a reaction time of DPPH with a sample, DPPH solvents and concentration, ratio between DPPH and an extract, etc.), the methodology of the evaluation of antioxidant capacity of different origin extracts by DPPH radical scavenging assay was developed. Ascorbic acid (AA) was used as standard antioxidant and the correlation between the percentage of DPPH scavenging and AA concentration was determined at two different initial absorbances of DPPH solution. Average concentration of AA which inhibited 50% of DPPH radicals (IC 50 ) was equal to 156.0 - 171.26 µg.mL -1 . The reaction kinetics of DPPH inhibition by bee bread and safflower extracts was described by the curves of the dependence of the total antioxidant activity on time with squared correlation coefficients (R 2 ) in the range of 0.89 - 0.98. The reaction times for these extracts were from 40 to 70 min at the correct ratio of volumes between the tested extracts and a DPPH solution. These studies demonstrated that the extracts obtained from bee bread of 2016 year of pollen collection had significantly higher the total antioxidant activity compared with the extracts of bee bread of pollen collection of 2015 considering the ratio of bee bread and the solvent in the extracts and volume of the extract for the procedure. This fact is explained not only botanical origin bun also the time of the storage of bee bread before the preparation of extracts. There was not found significant differences in the total antioxidant activity of extracts from flowers of safflower sown in fall and in spring. Antioxidant activity of the extracts from leaves of spring sown safflower was higher compared with the total antioxidant activity of the extracts from fall sown plants. Ascorbic acid equivalents of the tested extracts could be ranged as follows: bee bread of 2016 pollen collection >bee bread of 2015 pollen collection >leaves of safflower spring sown >flowers of safflower spring sown >flowers of safflower fall sown >leaves of safflower fall sown.

Morphological characteristics and determination of volatile organic compounds of Diospyros virginiana L. genotypes fruits

The aim of this study was to determine morphometric differences of fruits and seeds between 19 selected American persimmon ( Diospyros virginiana L.) genotypes. The genotypes of American persimmon have been growing more than 15 years in Forest-Steppe of Ukraine in the M.M. Gryshko National Botanical Garden of NAS of Ukraine. They are well adapted to the climatic and soil conditions. The fruits were collected at the period of their full maturity (October). The population differs in a weight, shape, size and color of fruits and seeds. Their morphometric parameters were following: fruit weight from 2.30 to 81.30 g, fruit length from 8.84 to 49.73 mm, fruit width from 12.84 to 55.34 mm, seed weight from 0.1 to 1.0 g, seed length from 8.30 to 20.88 mm, fruit width from 7.04 to 14.88 mm, seed thickness from 1.98 to 7.09 mm and number of seeds in the fruit from 1 to 8. Partenocarpic fruits are found in some genotypes. The shape indexes of fruits and seeds were found ranging from 0.74 to 1.10 and from 1.01 to 1.77, respectively. During the analysis of qualitative composition and quantitative content of volatiles of fruits from the 4 perspective genotypes of Diospyros virginiana 106 compounds were detected. From them 83 compounds were identified. The identified compounds belong to alcohols, saturated and unsaturated aldehydes, ketons, fatty acids, esters, terpenoids etc. The fruits are rich in fat acids. The outcome of the research points to the fact that the genepool of Ukrainian Diospyros virginiana is a rich source of genetic diversity and might be used in selection for creation a new genotypes and cultivars.

Morphological characteristics for fruits of aronia mitschurinii A.K. Skvortsov & Maitul.

The aim of this study was to determine morphometric characteristics of fruits within some phenotypes of Aronia mitschurinii A.K. Skvortsov & Maitul. Their morphometric parameters were following: weight from 0.75 g (AM-03) to 1.52 g (AM-04), length from 9.46 mm (AM-03) to 12.73 mm (AM-04), diameter from 10.49 mm (AM-03) to 13.73 mm (AM-04), fruits number in the corymb from 11.33 (AM-07) to 20.13 (AM-03), cumulative weight of fruits in the corymb from 10.42 g (AM-07) to 21.73 g (AM-04), volume of fruits from 0.55 (AM-03) to 1.26 (AM-04) cm 3 . The shape index of the fruits was found in the range of 0.87 (AR-01, AR-05, AR-07) to 0.93 (AM-02). The analysis of coefficient of variation showed the difference of variability in morphological characteristics between Aronia mitschurinii samples. Data showed that the most variability of important selection characteristics are the average cumulative mass of fruits in a сorymb - from 12.34 (AM-03) to 38.61 (AM-02) % and fruit number of fruits in the сorymb - from 14.56 (AM-03) to 36.88 (AM-02) %. The other characteristics are more or less stable. The introduction population of the Aronia mitschurinii , was created in the M.M. Gryshko National Botanical Garden in Kyiv, has a sufficient potential for successful selection work.

Pollen Grain Morphological Characteristics Of American Persimmon (Diospyros Virginiana L.)

151 POLLEN GRAIN MORPHOLOGICAL CHARACTERISTICS OF AMERICAN PERSIMMON (DIOSPYROS VIRGINIANA L.) Grygorieva Olga*, Motuleva Svetlana, Nikolaieva Nataliia, Klymenko Svitlana, Schubertová Zuzana, Brindza Jan M.M. Gryshko National Botanical Garden of Ukraine of National Academy of Sciences, Kyiv, Ukraine Federal State Budgetary Scientific Institution All-Russian Horticultural Institute for Breeding, Agrotechnology and Nursery, Moscow, Russia Faculty of Agrobiology and Food Resourses, Slovak University of Agriculture in Nitra, Slovak Republic

Morphological and antioxidant characteristics of quince (Cydonia oblonga Mill.) and chinese quince fruit (Pseudocydonia sinensis Schneid.)

Quince ( Cydonia oblonga Mill.) is a fruit species, whose fruits have a high therapeutic value and therefore are used in many countries in traditional medicine. Chinese quince ( Pseudocydonia sinensis Schneid.) is a lesser used species, although it is a relative of quince. The aim of the study was to evaluate some morphological characters of both kinds of fruit and antioxidant activity of morphological parts of the fruit. For these experiments, two genotypes were used from each species growing in the Arboretum Mlyňany (Slovakia). We determined the antioxidant activity of different parts by the DPPH method. In the genotypes from the evaluated species C. oblonga/P. sinensis we determined the average weight of the fruit in the fresh condition to be in the range 147.61 - 253.27 g / 197.85 - 466.38 g, the exocarp weight 28.50 - 43.89 g / 24.85 - 45.10 g, the mesocarp weight 116.36 - 204.99 g / 160.30 - 389.80 g, the seed weight 1.05 - 1.54 g / 9.22 - 17.42 g, the height of fruit 74.09 - 80.88 mm / 98.06 - 124.48 mm, average of fruit 60.11 - 81.51 mm / 62.33 - 88.64 mm. In aqueous extracts we determined antioxidant activity of the species C. oblonga / P. sinensis in dry exocarp in the range 43.52 - 67.73% / 52.76 - 82.20%, in fresh mesocarp 7.36 -14.78% / 15.30 - 23.50%, in dry mesocarp 30.92 - 41.30% / 41.68 - 50.15% and dry endocarp 55.19 - 76.44% / 91.20 - 92.72%. We determined antioxidant activity in methanolic extracts of the species C. oblonga / P. sinensis in dry exocarp in the range 93.29 - 93.32% / 91.87 - 93.25%, in fresh mesocarp 10.29 - 36.0% / 17.10 - 17.11%, in dry mesocarp 54.55 - 74.11% / 80.39 - 84.11% and in dry endocarp 95.14 - 95.39% / 94.97 - 95.62%. Results document that the fruits of both species can be practically used in the preparation of many dishes, while they can be used as raw material for pharmaceutical and cosmetic use.