Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Olga M. Tsivileva is active.

Publication


Featured researches published by Olga M. Tsivileva.


Applied Biochemistry and Microbiology | 2005

Effect of culture medium composition on the activity of extracellular lectins of Lentinus edodes

Olga M. Tsivileva; V. E. Nikitina; Lidiya V. Garibova

The time course of lectin production in culture liquid of the basidial fungus Lentinus edodes strain F-249 in different media under submerged culture conditions was studied. The activity of agglutinins depended on the ratio between carbon and nitrogen sources and the pH of the culture medium. The lectin activity in culture medium was maximal when the fungus was grown in a medium containing L-arabinose as a source of carbon and L-asparagine as a source of nitrogen (C:N ratio, (9.5–12):1) on day 15–18 of culturing at pH 8.0–9.0.


Biochemistry | 2008

Isolation and characterization of Lentinus edodes (Berk.) singer extracellular lectins

Olga M. Tsivileva; V. E. Nikitina; Ekaterina A. Loshchinina

Lectin preparations have been isolated and purified from the culture liquid of the xylotrophic basidiomycete Lentinus edodes (Berk.) Singer [Lentinula edodes (Berk.) Pegler]. The culture of L. edodes F-249 synthesizes two extracellular lectins different in composition and physicochemical properties. Extracellular lectin L1 from L. edodes is a glycoprotein of mono-subunit structure with molecular weight of 43 kD. L1 is comprised of 10.5 ± 1.0% (w/w) carbohydrates represented by glucose (Glc). Extracellular lectin L2 is a proteoglycan of mono-subunit structure with molecular weight of 37 kD. L2 is comprised of 90.3 ± 1.0% (w/w) carbohydrates represented by Glc (73% of the total mass of the carbohydrate moiety of the lectin molecule) and galactose (Gal) (27% of the total mass of the carbohydrate part of the lectin molecule). The content of Asn in L2 is high, i.e. 42% (w/w) of total amino acids. This fact along with the composition of the carbohydrate part of the molecule (Glc + Gal) allows one to assign L2 to N-asparagine-bound proteins. Both lectins are specific to D-Gal and lactose (Lac) at an equal for L1 and L2 minimal inhibiting concentration of these carbohydrates (2.08 mM Gal and 8.33 mM Lac). Other carbohydrates to which the lectins show affinity are different for the two lectins: Rha (4.16 mM) for L1 and Ara (4.16 mM) and mannitol (8.33 mM) for L2. The purified extracellular lectins of L. edodes are highly selective at recognition of definite structures on the surface of trypsinized rabbit erythrocytes and do not react with the erythrocytes of other animals and humans.


Microbiology | 2000

Hemagglutinating activity of the fungus Lentinus edodes (Berk.) sing [Lentinus edodes (Berk.) Pegler]

Olga M. Tsivileva; V. E. Nikitina; L. V. Garibova; L. A. Zav'yalova; V. V. Ignatov

The hemagglutinating (HA) activity of the submerged mycelium and the culture liquid (CL) of four strains ofLentinus edodes was studied. The HA activity of the CLs proved to be much higher than that of mycelia. The carbohydrate specificity of fungal agglutinating factors was determined. HA activity was investigated as a function of the inoculum size, cultivation temperature, and culture age. The agglutinating activity of different morphogenetic structures ofL. edodes F-249, including mycelium, brown mycelial mat (MM), primordia, and fruiting bodies, was studied. MM was found to possess the maximum HA activity, which can be explained by the possible involvement of agglutinins in the formation of MM, which is composed of glued hyphae.


Online Information Review | 2006

A brief guide to mycology web resources

Alexei N. Pankratov; Olga M. Tsivileva; V. E. Nikitina

Purpose – The purpose of this paper is to summarize the information about web resources in the area of mycology, one of the most important spheres of biology.Design/methodology/approach – The information on mycological web resources is systematised. Scientific journals, newsletters and magazines of mycological scope, along with their publishers, are presented.Findings – It is of use to biologists, chemists and other scientists and teachers working in the area of mycology, as well as scientists, who profess to disseminate scientific knowledge, to avail themselves of the myriad information sources on offer.Originality/value – This is possibly the first attempt to embrace comprehensively and in minute detail the vast field of information on the sources of contemporary mycology and its representative bodies.


Microbiology | 2004

Relationship between the Molecular Structure of the Nitrogen Source and the Activity of the Extracellular Lectins of Lentinus edodes (Berk.) Sing [Lentinula edodes (Berk.) Pegler] upon Submerged Cultivation

Olga M. Tsivileva; A. N. Pankratov; V. E. Nikitina; L. V. Garibova

The activity of the extracellular lectins of Lentinus edodes (Berk.) Sing [Lentinula edodes (Berk.) Pegler] and the formation of a pigmented mycelial film by this fungus upon submerged cultivation in a synthetic medium were found to depend on the presence of some amino acids (particularly, asparagine) and Ca2+ and Mn2+ ions in the medium. Quantum-chemical calculations suggest that the different character of the interaction of amino acids with the aforementioned ions is due to differences in the hydrophobicity of the amino acids rather than to differences in the electron structure of the amino acid zwitterions.


Mycological Progress | 2010

Extracellular protein production and morphogenesis of Lentinula edodes in submerged culture

Olga M. Tsivileva; Alexei N. Pankratov; V. E. Nikitina

Along with a brief review of Lentinula edodes (shiitake mushroom) submerged cultivation history within the framework of important extracellular proteins biosynthesis, this study contains the authors’ own results. The possibility of regulating the lectin activity of shiitake using the synthetic components is shown. The time course of lectin production in culture liquid of L. edodes in different media under submerged culture conditions was studied. The activity of agglutinins depended on the ratio between carbon and nitrogen sources and the pH of the culture medium. A relationship between the chemical composition of nutrient medium, the activity of extracellular lectins of L. edodes, and the formation of pigmented mycelial film in liquid culture has been found. The formulation of medium, on which the brown mycelial film appears in several days of submerged cultivation, is proposed. The results obtained make a contribution to the present notion of biochemical processes that give rise to the occurrence of the aforesaid morphological structure of shiitake. Finally, two extracellular lectins from the submerged culture of L. edodes have been isolated and purified to homogeneity. Their physicochemical properties and composition have been studied.


Journal of Biomolecular Structure & Dynamics | 2011

Compounds of the 1,5-di(4-R-phenyl)-3-selenopentanediones-1,5 series interaction with the Basidiomycete Lentinula edodes, lectins: Computations and Experiment

Alexei N. Pankratov; Olga M. Tsivileva; Boris I. Drevko; V. E. Nikitina

Abstract The role of spatial and electron structure, hydrophobic properties and concentration of organoselenium compounds on their interaction with fungal metabolites - extracellular lectins of Lentinula edodes, (shiitake mushroom) has been considered. By the hybrid method of density functional theory at the B3LYP/6-31G(d,p) theory level, spatial and electronic structure of the 1,5-diphenyl-3-selenopentanedione-1,5 (preparation DAPS-25), 1,5-di(4-methoxyphenyl)-3-selenopentanedione-1,5 and 1,5-di(4-ethoxyphenyl)-3-seleno- pentanedione-1,5 molecules has been studied. The above molecules have been stated to be substantially similar to each other by their electronic and spatial characteristics. By means of the QSAR properties evaluation by the atomic-additive schemes, it has been shown that the molecules of the preparation DAPS-25, its dimethoxy- and diethoxy-substituted are close to each other by the hydrophilic-lipophilic balance, whereas di-n,-octoxy derivative DAPS-25 is explicitly hydrophobic. The hemagglutinating activity of lectins in the presence of the preparation DAPS-25 and its alkyloxy-substituted increases, therewith the most effective addition is 1,5-di(4-ethoxyphenyl)-3-selenopentanedione-1,5. Apparently, the greater effectiveness of the said substance compared to DAPS −25 is caused by the formation of hydrogen bonds with a participation of unshared electron pairs of oxygen atoms from the ethoxy groups and mobile hydrogen atoms from the OH groups of glycoconjugates on erythrocytes surface. The positive effect of 1,5-di(4-n-octoxyphenyl)-3-selenopentanedione-1,5 is not so prominent, since the enlarged alkyl chain shields the aromatic fragments of organoselenium molecule participating in the binding with lectin.


Applied Biochemistry and Microbiology | 2008

Activity of Lentinus edodes intracellular lectins at various developmental stages of the fungus

E. P. Vetchinkina; V. E. Nikitina; Olga M. Tsivileva; Lidiya V. Garibova

We studied changes of the hemagglutinating activity of intracellular lectins of the basidiomycete Lentinus edodes (shiitake) at various stages of its morphogenetic development depending on erythrocyte type, growth medium, and lectin purification degree. Under certain experimental conditions, the specific lectin activity at the brown mycelium film stage exceeded the corresponding value for nonpigmented mycelium. The sensitivity of the lectins towards trypsin-treated rabbit erythrocytes was no less than a hundredfold higher than towards any other erythrocyte type studied. The general regularities of specific activity change did not depend on nutrient medium composition. With purification of intracellular shiitake lectins, their sensitivity to human erythrocytes decreased seventyfold or more, whereas their sensitivity to rabbit erythrocytes increased by the same factor.


Microbiology | 2005

Relationship between the Carbohydrate Specificity of Lectins and the Carbohydrate Composition of the Lentinus edodes (Berk.) Sing [Lentinula edodes (Berk.) Pegler] Mycelium at Different Stages of Its Morphogenesis

Olga M. Tsivileva; V. E. Nikitina; O. E. Makarov; L. V. Garibova

The specificity of lectins from the basidiomycete Lentinus edodes (Berk.) Sing [Lentinula edodes (Berk.) Pegler] during solid-state cultivation was found to be maximum to carbohydrates detected in the pyri-dine-soluble fraction of mycelium during the brown mycelial film stage. The carbohydrate composition of the mycelium (i.e., its content of maltose, rhamnose, mannitol, and inositol) was found to be different upon normal fruiting body formation and when the nonpigmented mycelium produced defective fruiting bodies.


Applied Biochemistry and Microbiology | 2012

Auxin synthesis by the higher fungus Lentinus edodes (Berk.) sing in the presence of low concentrations of indole compounds

Olga M. Tsivileva; Ekaterina A. Loshchinina; O. E. Makarov; V. E. Nikitina

The auxin formation in a submerged culture of the xylotrophic basidiomycete Lentinus edodes (Berk.) Sing (Lentinula edodes (Berk.) Pegler) (shiitake) is studied. Biologically active substances of an indole nature are identified, “the effect of small doses” of which lies in not only the stimulation of growth of the mycelium (indole-3-acetic acid, 2 × 10−7–2 × 10−4 g/l), but also in the induction of tryptophan-independent paths of auxin biosynthesis. The above-mentioned path is realized in the presence of exogenous indole (1 × 10−3–1 × 10−4 g/l), as well as while inducing the biosynthesis of indole-3-acetic acid by its microadditives (1 × 10−5−1 × 10−8 g/l), and is accompanied by the formation of anthranilic acid (up to 1.5 mg/l). Induction of the generative development stage of shiitake by indole derivatives is revealed. It was found that among the studied compounds only indoleacetamide at a concentration of an order of ×10−4 g/l in the culture fluid of L. edodes had a pronounced stimulatory effect on the formation of shiitake’s brown mycelial film.

Collaboration


Dive into the Olga M. Tsivileva's collaboration.

Top Co-Authors

Avatar

V. E. Nikitina

Russian Academy of Sciences

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar

L. V. Garibova

Russian Academy of Sciences

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Alla Perfileva

Russian Academy of Sciences

View shared research outputs
Top Co-Authors

Avatar

O. E. Makarov

Russian Academy of Sciences

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar

V. V. Ignatov

Russian Academy of Sciences

View shared research outputs
Researchain Logo
Decentralizing Knowledge