Olga Maria Silvério Amâncio
Federal University of São Paulo
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Revista Paulista De Pediatria | 2008
Fabíola Isabel Suano de Souza; Olga Maria Silvério Amâncio; Roseli Oselka Saccardo Sarni; Tassiana Sacchi Pitta; Ana Paula Fernandes; Fernando Luiz Affonso Fonseca; Sonia Hix
OBJECTIVE: To evaluate the prevalence of altered hepatic ultrasound and elevated serum alanine aminotransferase (ALT) in overweight and obese children, as well as to relate them to lipid peroxidation, lipid profile and insulin resistance. METHODS: A prospective, controlled, cross-sectional study was performed with 67 overweight and obese children (38 female, mean age of 8,6 years) paired by gender and age with 65 eutrophic controls. The following parameters were evaluated: lipid profile (LDL-c, HDL-c, triglycerides), ALT, lipid peroxidation measured by thiobarbituric acid reaction substance (TBARS), serum glucose and insulin resistance (Homeostasis Model Assessment). Hepatic steatosis was evaluated by ultrasound by a single professional and classified as diffuse mild, moderate or severe. RESULTS: Elevated ALT (>40 U/L) was observed in 3% and altered ultrasound in 57.4% of the overweight/obese children. Obese/overweight children showed a higher percentage of ALT >18 U/L (OR 4.2, p=0.0006) and hypertriglyceridemia (OR 4.2, p 18 U/L. CONCLUSIONS: The frequency of altered ALT (>40U/L) was low and of altered hepatic ultrasound was high. There was no association between fatty liver at ultrasound with nutritional status or ALT levels. Elevated triglycerides were associated with higher levels of ALT.
Brazilian Journal of Medical and Biological Research | 2003
M. Catani; Olga Maria Silvério Amâncio; Ulysses Fagundes-Neto; Mauro Batista de Morais
The objective of the present study was to determine the effect of cellulose on intestinal iron absorption in rats during recovery from iron deficiency anemia. Twenty-one-day-old male Wistar-EPM rats were fed an iron-free ration for two weeks to induce anemia. At 5 weeks of age, the rats were divided into two groups (both groups receiving 35 mg of elemental iron per kg diet): cellulose group (N = 12), receiving a diet containing 100 g of cellulose/kg and control (N = 12), receiving a diet containing no cellulose. The fresh weight of the feces collected over a 3-day period between the 15th and 18th day of dietary treatment was 10.7 +/- 3.5 g in the group receiving cellulose and 1.9 +/- 1.2 g in the control group (P<0.001). Total food intake was higher in the cellulose group (343.4 +/- 22.0 g) than in the control (322.1 +/- 13.1 g, P = 0.009) during the 3 weeks of dietary treatment. No significant difference was observed in weight gain (cellulose group = 132.8 +/- 19.2, control = 128.0 +/- 16.3 g), hemoglobin increment (cellulose group = 8.0 +/- 0.8, control = 8.0 +/- 1.0 g/dl), hemoglobin level (cellulose group = 12.3 +/- 1.2, control = 12.1 +/- 1.3 g/dl) or in hepatic iron levels (cellulose group = 333.6 +/- 112.4, control = 398.4 +/- 168.0 g/g dry tissue). We conclude that cellulose does not adversely affect the regeneration of hemoglobin, hepatic iron level or the growth of rats during recovery from iron deficiency anemia.
Revista Panamericana De Salud Publica-pan American Journal of Public Health | 2009
Francisco Plácido Nogueira Arcanjo; Vicente P. T. Pinto; Maria R. Arcanjo; Márcia R. Amici; Olga Maria Silvério Amâncio
The study objective was to measure the effect of consumption of a beverage mixed with a high-iron sweetener (evaporated sugarcane juice known as rapadura) on hemoglobin levels in preschool children, and to compare it with the effect of consuming the same beverage sweetened with refined sugar. Research consisted of a 12-week randomized, controlled double-blind trial conducted in 2007 at a state-run school in Sobral, Brazil, among children aged 2-3 years. The study sample was divided into two groups-one consuming cashew juice mixed with 25 g of rapadura and 40 mg of ascorbic acid (per 200-mL serving), and another consuming the same quantity of juice and ascorbic acid sweetened with 25 g of standard refined sugar. A significant statistical increase in hemoglobin was observed in the group consuming the rapadura-fortified beverage. It was therefore concluded that consumption of rapadura increased hemoglobin and thus reduced iron deficiency anemia in preschool children.
Jornal De Pediatria | 2004
Karine de Cássia Freitas; Maria Eugênia F. de A. Motta; Olga Maria Silvério Amâncio; Ulysses Fagundes Neto; Mauro Batista de Morais
OBJECTIVE To compare the effect of soy polysaccharide on fecal weight and humidity in growing rats in relation to cellulose and a Soy formula without dietary fiber. METHODS Twelve, 21-day-old, Wistar male rats, were distributed into three groups and fed AIN-93G ration with varying amounts of dietary fiber: Polysaccharide Group, 5% fiber in the form of soy polysaccharide; Soy Formula Group, 5% soy formula without fiber; and Cellulose Group, 5% fiber in cellulose form. Feces were collected for three 72-hour periods beginning on days 7, 17 and 27. They were weighed while humid and then dried at 105 degrees C until a constant weight. The humidity was calculated using the formula [(humid fecal weight - dry fecal weight)/humid fecal weight] X 100. RESULTS The total humid fecal weight for Polysaccharide, Soy Formula and Cellulose groups was: 17.372-/+4.743 g; 6.045-/+0.619 g; and 16.012-/+2.600 g, (p = 0.001), respectively. There was no statistically significant difference between the Cellulose and Polysaccharide groups, but a statistically significant difference was observed between theses two groups and the soy formula group. Values for dry weight, in the same order, were: 6.463-/+1.177 g; 2.909-/+0.277 g; and 10.068-/+1.085 g, (p < 0.001), with a statistically significant difference between each of the groups. CONCLUSION Animals that received soy formula presented lower humid and dried fecal weight than the other two groups, while the soy polysaccharide caused higher fecal humidity than did cellulose, probably due to greater fermentation.
Jornal De Pediatria | 2015
Maisa de Lima Correia Silva; Patrícia da Graça Leite Speridião; Renata Marciano; Olga Maria Silvério Amâncio; Tânia Beninga de Morais; Mauro Batista de Morais
OBJECTIVE To compare body growth, weight, and fecal moisture in recently weaned rats fed exclusively on infant soy formula and soy-based beverage. METHODS Three similar groups were formed (n=10/group) consisting of weanling Wistar rats, maintained in metabolic cages. One group was fed soy protein-based beverage, another with soy-based infant formula, and another with cows milk infant formula (control group). Water and diet were offered ad libitum. Body weight and length were measured. Stool was collected for three consecutive days. RESULTS Weight and length were lower (p = 0.001; p = 0.001) in the groups receiving soy protein-based beverage (73.16 ± 5.74 g; 23.94 ± 1.04 cm) and soy-based formula (71.11 ± 5.84 g; 24.74 ± 0.60 cm) in relation to the group receiving cows milk formula (84.88 ± 9.75 g; 26.01 ± 0.91 cm). Fresh fecal weight was greater (p < 0.001) in the soy-based beverage (3.44 ± 0.48 g) than in the soy-based formula (0.79 ± 0.20 g) and cows milk-based formula (0.42 ± 0.17 g). Fecal moisture was higher (p < 0.001) in the group receiving soy protein-based beverage (47.28 ± 9.02%) and soy-based formula (37.21 ± 13.20%) than in the group receiving cows milk formula (22.71 ± 10.86%). CONCLUSION The growth of rats fed soy protein-based beverage and soy-based formula was lower than those fed cows milk-based formula. The soy protein-based beverage resulted in significant increase in fecal weight and moisture.
Revista De Nutricao-brazilian Journal of Nutrition | 2017
Maisa de Lima Correia Silva; Patrícia da Graça Leite Speridião; Renata Marciano; Olga Maria Silvério Amâncio; Tânia Beninga de Morais; Mauro Batista de Morais
Objetivo: Este estudo objetivou comparar a absorcao intestinal de ferro e calcio entre formulas infantis a base de leite de vaca e de soja em ratos recem-desmamados. Metodos: Vinte ratos machos Wistar, com 21 dias de vida e no primeiro dia do desmame foram utilizados neste experimento, divididos em dois grupos: um alimentado com formula infantil a base de proteina de soja e o outro com formula infantil a base de proteina do leite de vaca. Durante o periodo de estudo (10 dias consecutivos), os ratos receberam dieta e agua ad libitum. Hematocrito e hemoglobina foram mensurados no primeiro, quinto e decimo dia pelo metodo de Wintrobe e da cianometaemoglobina. As fezes e a urina foram recolhidas a partir do quinto dia durante durante tres dias consecutivos. No decimo dia, o conteudo de ferro hepatico tambem foi analisado. O conteudo de ferro hepatico e a analise de ferro e calcio nas fezes e na urina foram realizados utilizando espectrofotometro de absorcao atomica. Aos 31 dias de vida, os animais foram anestesiados com ketamina e xilasina e sacrificados por exsanguinacao da veia vaca. Resultados: A concentracao final de hemoglobina no grupo formula infantil a base de soja e formula infantil a base de leite de vaca foram 10,3±1,3g/dL e 10,9± 1,0g/dL (p=0,310), respectivamente. A absorcao aparente de ferro e calcio foi de: 73,4±10,2% e 70,2±9,5%, para o primeiro grupo; e 97,2±0,7% e 97,6±1,0%, para o segundo (p=0,501; p=0,290). A retencao aparente de calcio foi: 88,4±2,2% e 88,6±2,6% (p=0,848). O teor de ferro hepatico foi: 522,0±121,1µg/g e 527,8±80,5µg/g (p=0,907), respectivamente. Conclusao: A absorcao intestinal de ferro e calcio da formula infantil a base de soja e similar a da formula infantil a base de leite em ratos recem-desmamados.
Journal of Tropical Pediatrics | 2008
Francisco Plácido Nogueira Arcanjo; Vicente P. T. Pinto; M. R. Coelho; Olga Maria Silvério Amâncio; Silvia Maria Meira Magalhães
Nutrition | 2011
Mônica Lisboa Chang Wayhs; Mauro Batista de Morais; Ubiratan Fabres Machado; Silvia Modesto Nassar; Ulysses Fagundes Neto; Olga Maria Silvério Amâncio
Jornal De Pediatria | 2004
Mauro Batista de Morais; Karine de Cássia Freitas; Maria Eugênia Farias Almeida Motta; Olga Maria Silvério Amâncio; Ulysses Fagundes-Neto
The FASEB Journal | 2015
Roberta Soares Lara Cassani; Olga Maria Silvério Amâncio; Bruno Geloneze; José Carlos Pareja