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Featured researches published by Olga Torres.
Molecular Nutrition & Food Research | 2012
Ronald T. Riley; Olga Torres; Jency L. Showker; Nicholas C. Zitomer; Jorge Matute; Kenneth A. Voss; Janee Gelineau-van Waes; Joyce R. Maddox; Simon G. Gregory; Allison E. Ashley-Koch
SCOPE Fumonisins (FB) are mycotoxins found in maize. The purpose of this study was to (i) determine the relationship between FB(1) , FB(2) , and FB(3) intake and urinary excretion in humans, (ii) validate a method to isolate urinary FB on C(18) -SPE cartridges for international shipment, and (iii) test the method using samples from Guatemala. METHODS AND RESULTS Volunteers (n = 10) consumed 206 grams/day of tortillas and biscuits prepared from masa flour and a product containing maize flour. Volunteers estimated their daily urine output and samples were analyzed for FB(1) , FB(2) , and FB(3) and hydrolyzed FB(1) . Only FB(1) was detected in urine suggesting lower absorption of FB(2) and FB(3) . Excretion was highly variable peaking soon after consumption began and decreasing rapidly after consumption stopped. Within 5 days after consumption ended, FB(1) was not detected in urine. In a study with eight volunteers, the average total urinary FB(1) was 0.5% of the intake. FB(1) was detected in 61% (107/177) of the samples collected in Guatemala. CONCLUSION The results support the use of urinary FB(1) to assess ongoing exposure in population-based studies. However, relating the FB(1) concentration in urine to dietary intake of FB by individual subjects will be complicated due to interindividual variability and the rapidity of clearance.
Molecular Nutrition & Food Research | 2014
Olga Torres; Jorge Matute; Janee Gelineau-van Waes; Joyce R. Maddox; Simon G. Gregory; Allison E. Ashley-Koch; Jency L. Showker; Nicholas C. Zitomer; Kenneth A. Voss; Ronald T. Riley
SCOPE Fumonisin (FB) intake can be high when maize is a dietary staple. We determined (i) urinary FB (UFB) in women consuming maize in high- and low-exposure communities in Guatemala, (ii) the FB levels in maize, (iii) the relationship between UFB and FB intake, and (iv) the relative excretion of UFB1 , UFB2 , and UFB3 . METHODS AND RESULTS Urine and maize were analyzed for FB for 1 year in three departments. Maize consumption was estimated by an interview questionnaire. Fumonisin B1 , B2 , and B3 (FB1 , FB2 and FB3 ), were detected in 100% of maize samples. FB1 in maize and urine was significantly higher in Jutiapa compared to Chimaltenango or Escuintla. The FB intake paralleled UFB1 in a dose-dependent manner but UFB1 was present in much higher levels than UFB2 or UFB3 compared to maize. CONCLUSION In Jutiapa, agroecological conditions favored FB production. UFB1 mirrored the estimated FB intake. UFB1 > 0.1 ng/mL resulted in a dose-dependent increase in the risk of exceeding FB intake of 2 μg/kg b.w./day compared to women with no detectable UFB1 . More than 50% exceeded 2 μg/kg b.w./day when UFB1 was >0.5 ng/mL. UFB2 and UFB3 were rarely detected confirming that FB1 is either absorbed better or preferentially excreted in urine.
World Mycotoxin Journal | 2015
Olga Torres; Jorge Matute; J. Gelineau-van Waes; Joyce R. Maddox; Simon G. Gregory; Allison E. Ashley-Koch; Jency L. Showker; Kenneth A. Voss; Ronald T. Riley
Co-occurrence of fumonisin B1 (FB1) and aflatoxin B1 (AFB1) in maize has been demonstrated in many surveys. Combined-exposure to FB1 and AFB1 was of concern to the Joint FAO/WHO Expert Committee on...
Molecular Nutrition & Food Research | 2015
Ronald T. Riley; Olga Torres; Jorge Matute; Simon G. Gregory; Allison E. Ashley-Koch; Jency L. Showker; Trevor R. Mitchell; Kenneth A. Voss; Joyce R. Maddox; Janee Gelineau-van Waes
SCOPE Fumonisin (FB) occurs in maize and is an inhibitor of ceramide synthase (CerS). We determined the urinary FB1 (UFB1 ) and sphingoid base 1-phosphate levels in blood from women consuming maize in high and low FB exposure communities in Guatemala. METHODS AND RESULTS FB1 intake was estimated using the UFB1 . Sphinganine 1-phosphate (Sa 1-P), sphingosine 1-phosphate (So 1-P), and the Sa 1-P/So 1-P ratio were determined in blood spots collected on absorbent paper at the same time as urine collection. In the first study, blood spots and urine were collected every 3 months (March 2011 to February 2012) from women living in low (Chimaltenango and Escuintla) and high (Jutiapa) FB exposure communities (1240 total recruits). The UFB1 , Sa 1-P/So 1-P ratio, and Sa 1-P/mL in blood spots were significantly higher in the high FB1 intake community compared to the low FB1 intake communities. The results were confirmed in a follow-up study (February 2013) involving 299 women living in low (Sacatepéquez) and high (Santa Rosa and Chiquimula) FB exposure communities. CONCLUSIONS High levels of FB1 intake are correlated with changes in Sa 1-P and the Sa 1-P/So 1-P ratio in human blood in a manner consistent with FB1 inhibition of CerS.
Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment | 2015
Ronald T. Riley; Jency L. Showker; Christine M. Lee; Cody E. Zipperer; Trevor R. Mitchell; Kenneth A. Voss; Nicholas C. Zitomer; Olga Torres; Jorge Matute; Simon G. Gregory; Allison E. Ashley-Koch; Joyce R. Maddox; Nicole M. Gardner; Janee Gelineau-van Waes
Fumonisins (FB) are mycotoxins found in maize. They are hypothesised risk factors for neural tube defects (NTDs) in humans living where maize is a dietary staple. In LM/Bc mice, FB1-treatment of pregnant dams induces NTDs and results in increased levels of sphingoid base 1-phosphates in blood and tissues. The increased level of sphingoid base 1-phosphates in blood is a putative biomarker for FB1 inhibition of ceramide synthase in humans. Collection of blood spots on paper from finger sticks is a relatively non-invasive way to obtain blood for biomarker analysis. The objective of this study was to develop and validate in an animal model, and ultimately in humans, a method to estimate the volume of blood collected as blood spots on absorbent paper so as to allow quantification of the molar concentration of sphingoid base 1-phosphates in blood. To accomplish this objective, blood was collected from unexposed male LM/Bc and FB1-exposed pregnant LM/Bc mice and humans and applied to two types of absorbent paper. The sphingoid base 1-phosphates, absorbance at 270 nm (A270), and total protein content (Bradford) were determined in the acetonitrile:water 5% formic acid extracts from the dried blood spots. The results show that in both mouse and human the A270, total protein, and blood volume were closely correlated and the volume of blood spotted was accurately estimated using only the A270 of the extracts. In mouse blood spots, as in tissues and embryos, the FB1-induced changes in sphingolipids were correlated with urinary FB1. The half-life of FB1 in the urine was short (<24 h) and the elevation in sphingoid base 1-phosphates in blood was also short, although more persistent than the urinary FB1.
Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment | 2006
Ronald T. Riley; Olga Torres; E. Palencia
Fumonisins are mycotoxins found in maize. In developing countries, the resources required for analysis are often lacking, and the shipping of maize between countries can be difficult since the importation of plant materials requires permits/inspection to prevent the entry of pests that frequently infest maize. A simple, safe and legal method for shipping maize extracts to the USA was needed to conduct a survey of fumonisins in Central America. The objective was to develop a method for isolating and shipping maize extracts for fumonisin analysis so as to facilitate a survey of fumonisin exposure. The results indicate that fumonisins in acetonitrile:water extracts of maize can be isolated on C18 cartridges, held for at least 3 days at 22°C and then an additional 4 days at 4°C before elution and analysis with no losses. This method allows the importation and analysis of maize samples from foreign locations without complications from international safety concerns.
Journal of Nutrition | 2004
W. F. O. Marasas; Ronald T. Riley; Katherine A. Hendricks; Victoria L. Stevens; Thomas W. Sadler; Janee Gelineau-van Waes; Stacey A. Missmer; Julio Cabrera; Olga Torres; Wentzel C. A. Gelderblom; Jeremy C. Allegood; Carolina Martínez; Joyce Maddox; J. David Miller; Lois Starr; M. Cameron Sullards; Ana Victoria Roman; Kenneth A. Voss; Elaine Wang; Alfred H. Merrill
Journal of Nutrition | 2003
Edwin R. Palencia; Olga Torres; Winston M. Hagler; Filmore I. Meredith; Lonnie D. Williams; Ronald T. Riley
Journal of Nutrition | 2007
Olga Torres; Edwin R. Palencia; Ligia Lopez de Pratdesaba; Rubén Grajeda; Mario Fuentes; Marcy C. Speer; Alfred H. Merrill; Kerry O'Donnell; Charles W. Bacon; Anthony E. Glenn; Ronald T. Riley
Archive | 2012
Waes Gelineau-van; Ronald T. Riley; Kenneth A. Voss; Jency L. Showker; Olga Torres; J. Matutue; Joyce R. Maddox; Mark A. Rainey; Nicole M. Gardner; Andrew J. Sachs; Simon G. Gregory; Allison E. Ashley-Koch; D. Krupp